Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations

Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedn...

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Main Authors:	Othman, Halimaton Saadiah, Osman, Mohamad, Zainuddin, Zarina
Format:	Article
Language:	English English English
Published:	Agriculture Faculty Brawijaya University 2018
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/64507/ http://irep.iium.edu.my/64507/ http://irep.iium.edu.my/64507/ http://irep.iium.edu.my/64507/1/64507_Genetic%20Variabilities%20of%20Stevia%20rebaudiana.pdf http://irep.iium.edu.my/64507/2/64507_Genetic%20Variabilities%20of%20Stevia%20rebaudiana_SCOPUS.pdf http://irep.iium.edu.my/64507/13/64507%20Genetic%20variabilities%20of%20Stevia%20rebaudiana%20Bertoni%20cultivated%20in%20Malaysia%20as%20revealed%20by%20morphological%2C%20chemical%20and%20molecular%20characterisations_wos.pdf

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spelling	iium-645072019-01-24T04:15:29Z http://irep.iium.edu.my/64507/ Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations Othman, Halimaton Saadiah Osman, Mohamad Zainuddin, Zarina Q Science (General) Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedness among stevia accessions in order to broaden the genetic basis for future stevia breeding. This study described morphological and chemical variations and investigates genetic relationships among stevia accessions derived from across Malaysia and Paraguay using inter simple sequence repeats (ISSR) markers. HPLC (high-performance liquid chromatography) analysis also revealed high variability with stevioside content between 4.54 % (Taman Pertanian) to 20.36 % (Bangi) and rebaudioside A content varied between 0.3 % (Nilai) to 2.04 % (MNQ). From 32 ISSR markers, a total of 332 bands were scored, of which 264 (78 %) were polymorphic. The dendrogram from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis separated 17 stevia accessions into 3 main groups. Rawang and Nilai were found to be closely related. The wide genetic variabilities among stevia accessions are a promising indicator towards the development of new stevia varieties. This valuable information will be able to assist parental selection in future stevia breeding programmes. Agriculture Faculty Brawijaya University 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64507/1/64507_Genetic%20Variabilities%20of%20Stevia%20rebaudiana.pdf application/pdf en http://irep.iium.edu.my/64507/2/64507_Genetic%20Variabilities%20of%20Stevia%20rebaudiana_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/64507/13/64507%20Genetic%20variabilities%20of%20Stevia%20rebaudiana%20Bertoni%20cultivated%20in%20Malaysia%20as%20revealed%20by%20morphological%2C%20chemical%20and%20molecular%20characterisations_wos.pdf Othman, Halimaton Saadiah and Osman, Mohamad and Zainuddin, Zarina (2018) Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations. AGRIVITA Journal of Agricultural Science, 40 (2). pp. 267-283. ISSN 2302-6566 E-ISSN 2477-8516 http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/1365/932 10.17503/agrivita.v40i2.1365
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Othman, Halimaton Saadiah Osman, Mohamad Zainuddin, Zarina Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations
description	Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedness among stevia accessions in order to broaden the genetic basis for future stevia breeding. This study described morphological and chemical variations and investigates genetic relationships among stevia accessions derived from across Malaysia and Paraguay using inter simple sequence repeats (ISSR) markers. HPLC (high-performance liquid chromatography) analysis also revealed high variability with stevioside content between 4.54 % (Taman Pertanian) to 20.36 % (Bangi) and rebaudioside A content varied between 0.3 % (Nilai) to 2.04 % (MNQ). From 32 ISSR markers, a total of 332 bands were scored, of which 264 (78 %) were polymorphic. The dendrogram from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis separated 17 stevia accessions into 3 main groups. Rawang and Nilai were found to be closely related. The wide genetic variabilities among stevia accessions are a promising indicator towards the development of new stevia varieties. This valuable information will be able to assist parental selection in future stevia breeding programmes.
format	Article
author	Othman, Halimaton Saadiah Osman, Mohamad Zainuddin, Zarina
author_facet	Othman, Halimaton Saadiah Osman, Mohamad Zainuddin, Zarina
author_sort	Othman, Halimaton Saadiah
title	Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations
title_short	Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations
title_full	Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations
title_fullStr	Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations
title_full_unstemmed	Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations
title_sort	genetic variabilities of stevia rebaudiana bertoni cultivated in malaysia as revealed by morphological, chemical and molecular characterisations
publisher	Agriculture Faculty Brawijaya University
publishDate	2018
url	http://irep.iium.edu.my/64507/ http://irep.iium.edu.my/64507/ http://irep.iium.edu.my/64507/ http://irep.iium.edu.my/64507/1/64507_Genetic%20Variabilities%20of%20Stevia%20rebaudiana.pdf http://irep.iium.edu.my/64507/2/64507_Genetic%20Variabilities%20of%20Stevia%20rebaudiana_SCOPUS.pdf http://irep.iium.edu.my/64507/13/64507%20Genetic%20variabilities%20of%20Stevia%20rebaudiana%20Bertoni%20cultivated%20in%20Malaysia%20as%20revealed%20by%20morphological%2C%20chemical%20and%20molecular%20characterisations_wos.pdf
first_indexed	2023-09-18T21:31:32Z
last_indexed	2023-09-18T21:31:32Z
_version_	1777412543924404224

Genetic variabilities of stevia rebaudiana bertoni cultivated in Malaysia as revealed by morphological, chemical and molecular characterisations

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