Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract

Medicinal plants are commonly used in complementary and alternative medicine to treat several chronic diseases. Gynura procumbens, Hibiscus sabdariffa and Clinacanthus nutans are believed to contain high level of antioxidants including phenolic acids that may contribute to antioxidant activities. Th...

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Main Authors: Haron, Normah, Muhamad Bunnori, Noraslinda, Md Zin, Noor Hasniza, Abdul Wahab, Widya, Abd Halim, Khairul Bariyyah, Mat Jusoh, Hanapi
Format: Conference or Workshop Item
Language:English
English
Published: 2016
Subjects:
Online Access:http://irep.iium.edu.my/64822/
http://irep.iium.edu.my/64822/1/Poster-Format-MRS%20KRD%282%29%20Dr.%20Normah%20Haron.pdf
http://irep.iium.edu.my/64822/17/64822%20Quantification%20of%20total%20phenolic.pdf
id iium-64822
recordtype eprints
spelling iium-648222019-08-23T07:21:25Z http://irep.iium.edu.my/64822/ Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract Haron, Normah Muhamad Bunnori, Noraslinda Md Zin, Noor Hasniza Abdul Wahab, Widya Abd Halim, Khairul Bariyyah Mat Jusoh, Hanapi QD Chemistry Medicinal plants are commonly used in complementary and alternative medicine to treat several chronic diseases. Gynura procumbens, Hibiscus sabdariffa and Clinacanthus nutans are believed to contain high level of antioxidants including phenolic acids that may contribute to antioxidant activities. Thus, the objectives of this study were to determine total phenolic content (TPC) and antioxidant scavenging capacity of these plants using three different extracts as well as to determine the relationship between TPC and antioxidant activity. The quantification of phenolic compound was conducted by analyzing the compounds at three different fractions (free, insoluble bound and soluble bound). Three replicates of the medicinal plants were extracted with 80% methanol (free fraction) and undergone hydrolysis with HCl (soluble bound) and NaOH (insoluble bound). The TPC was determined using Folin-Ciocalteau reagent while antioxidant radical scavenging capacities were analyzed using DPPH assay. Results showed that G. procumbens and C. nutans of the insoluble bound extracts had the highest amount of TPC compared to the free phenolic and soluble bound (p<0.05). Meanwhile, H. sabdariffa had the highest TPC value in soluble bound extract (7.63 + 0.28 mg GAE/g) compared to the free phenolic and insoluble bound extracts (2.78 + 0.20 mg GAE/g and 3.61 + 0.85 mg GAE/g, respectively) (p<0.05). The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts as determined by IC50 values was the highest in the insoluble bound extract followed by free phenolic and soluble bound extracts (p<0.05). In contrast, the soluble bound extract of H. sabdariffa showed the highest IC50 value followed by free phenolic and insoluble bound extracts. We also found strong correlations between TPC with antioxidant radical scavenging capacity. In conclusion, this finding suggests that these plants possess a good source of natural antioxidants, which could be used as an non-conventional substances in the management of inflammatory-related diseases. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/64822/1/Poster-Format-MRS%20KRD%282%29%20Dr.%20Normah%20Haron.pdf application/pdf en http://irep.iium.edu.my/64822/17/64822%20Quantification%20of%20total%20phenolic.pdf Haron, Normah and Muhamad Bunnori, Noraslinda and Md Zin, Noor Hasniza and Abdul Wahab, Widya and Abd Halim, Khairul Bariyyah and Mat Jusoh, Hanapi (2016) Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Haron, Normah
Muhamad Bunnori, Noraslinda
Md Zin, Noor Hasniza
Abdul Wahab, Widya
Abd Halim, Khairul Bariyyah
Mat Jusoh, Hanapi
Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
description Medicinal plants are commonly used in complementary and alternative medicine to treat several chronic diseases. Gynura procumbens, Hibiscus sabdariffa and Clinacanthus nutans are believed to contain high level of antioxidants including phenolic acids that may contribute to antioxidant activities. Thus, the objectives of this study were to determine total phenolic content (TPC) and antioxidant scavenging capacity of these plants using three different extracts as well as to determine the relationship between TPC and antioxidant activity. The quantification of phenolic compound was conducted by analyzing the compounds at three different fractions (free, insoluble bound and soluble bound). Three replicates of the medicinal plants were extracted with 80% methanol (free fraction) and undergone hydrolysis with HCl (soluble bound) and NaOH (insoluble bound). The TPC was determined using Folin-Ciocalteau reagent while antioxidant radical scavenging capacities were analyzed using DPPH assay. Results showed that G. procumbens and C. nutans of the insoluble bound extracts had the highest amount of TPC compared to the free phenolic and soluble bound (p<0.05). Meanwhile, H. sabdariffa had the highest TPC value in soluble bound extract (7.63 + 0.28 mg GAE/g) compared to the free phenolic and insoluble bound extracts (2.78 + 0.20 mg GAE/g and 3.61 + 0.85 mg GAE/g, respectively) (p<0.05). The antioxidant scavenging capacity of the G. procumbens and C. nutans extracts as determined by IC50 values was the highest in the insoluble bound extract followed by free phenolic and soluble bound extracts (p<0.05). In contrast, the soluble bound extract of H. sabdariffa showed the highest IC50 value followed by free phenolic and insoluble bound extracts. We also found strong correlations between TPC with antioxidant radical scavenging capacity. In conclusion, this finding suggests that these plants possess a good source of natural antioxidants, which could be used as an non-conventional substances in the management of inflammatory-related diseases.
format Conference or Workshop Item
author Haron, Normah
Muhamad Bunnori, Noraslinda
Md Zin, Noor Hasniza
Abdul Wahab, Widya
Abd Halim, Khairul Bariyyah
Mat Jusoh, Hanapi
author_facet Haron, Normah
Muhamad Bunnori, Noraslinda
Md Zin, Noor Hasniza
Abdul Wahab, Widya
Abd Halim, Khairul Bariyyah
Mat Jusoh, Hanapi
author_sort Haron, Normah
title Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_short Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_full Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_fullStr Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_full_unstemmed Quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
title_sort quantification of total phenolic contents and their antioxidant scavenging capacity in selected herbs extract
publishDate 2016
url http://irep.iium.edu.my/64822/
http://irep.iium.edu.my/64822/1/Poster-Format-MRS%20KRD%282%29%20Dr.%20Normah%20Haron.pdf
http://irep.iium.edu.my/64822/17/64822%20Quantification%20of%20total%20phenolic.pdf
first_indexed 2023-09-18T21:32:01Z
last_indexed 2023-09-18T21:32:01Z
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