The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study

The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasop...

Full description

Bibliographic Details
Main Authors:	Othman, Noor Syarmila, Leman, Wan Ishlah, Abdullah, Kahairi, Muhammad, Siti Aesah @ Naznin, Kaderi, Mohd Arifin
Format:	Article
Language:	English
Published:	IIUM Press, International Islamic University Malaysia 2018
Subjects:	RC Internal medicine
Online Access:	http://irep.iium.edu.my/64881/ http://irep.iium.edu.my/64881/ http://irep.iium.edu.my/64881/1/64881_The%20expression%20of%20microrna-744.pdf

id	iium-64881
recordtype	eprints
spelling	iium-648812018-07-31T01:31:21Z http://irep.iium.edu.my/64881/ The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study Othman, Noor Syarmila Leman, Wan Ishlah Abdullah, Kahairi Muhammad, Siti Aesah @ Naznin Kaderi, Mohd Arifin RC Internal medicine The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasopharynx tissue taken from Hospital Tengku Ampuan Afzan (HTAA), Kuantan and converted into cDNA. The level of miR-744 expression in the cDNA was quantified using quantitative reverse transcription polymserase chain reaction (RT-qPCR) technique. The expression level of SNORD48 was measured simultaneously for each sample, which served as endogenous control. The difference in the expression of miR-744 in NPC and normal nasopharynx tissue were analysed using relative quantification, 2-ΔΔCT. In this preliminary analysis, this study found that miR-744 was upregulated in NPC as compared to normal nasopharynx tissue by 2.5 fold changes, respectively suggesting it may involve in progression of tumour. However, the finding is not significant and may not accurately reflect the overall population, due to small sample size involved in the study. Findings from the current study suggest the potential of miR-744 to serve as useful diagnostic and prognostic biomarker in NPC. IIUM Press, International Islamic University Malaysia 2018-07-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64881/1/64881_The%20expression%20of%20microrna-744.pdf Othman, Noor Syarmila and Leman, Wan Ishlah and Abdullah, Kahairi and Muhammad, Siti Aesah @ Naznin and Kaderi, Mohd Arifin (2018) The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study. International Journal of Allied Health Sciences, 2 (1). pp. 191-203. E-ISSN 2600-8491 http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/113
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC Internal medicine
spellingShingle	RC Internal medicine Othman, Noor Syarmila Leman, Wan Ishlah Abdullah, Kahairi Muhammad, Siti Aesah @ Naznin Kaderi, Mohd Arifin The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
description	The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasopharynx tissue taken from Hospital Tengku Ampuan Afzan (HTAA), Kuantan and converted into cDNA. The level of miR-744 expression in the cDNA was quantified using quantitative reverse transcription polymserase chain reaction (RT-qPCR) technique. The expression level of SNORD48 was measured simultaneously for each sample, which served as endogenous control. The difference in the expression of miR-744 in NPC and normal nasopharynx tissue were analysed using relative quantification, 2-ΔΔCT. In this preliminary analysis, this study found that miR-744 was upregulated in NPC as compared to normal nasopharynx tissue by 2.5 fold changes, respectively suggesting it may involve in progression of tumour. However, the finding is not significant and may not accurately reflect the overall population, due to small sample size involved in the study. Findings from the current study suggest the potential of miR-744 to serve as useful diagnostic and prognostic biomarker in NPC.
format	Article
author	Othman, Noor Syarmila Leman, Wan Ishlah Abdullah, Kahairi Muhammad, Siti Aesah @ Naznin Kaderi, Mohd Arifin
author_facet	Othman, Noor Syarmila Leman, Wan Ishlah Abdullah, Kahairi Muhammad, Siti Aesah @ Naznin Kaderi, Mohd Arifin
author_sort	Othman, Noor Syarmila
title	The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_short	The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_full	The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_fullStr	The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_full_unstemmed	The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study
title_sort	expression of microrna-744 (mir-744) in nasopharyngeal carcinoma: a preliminary study
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/64881/ http://irep.iium.edu.my/64881/ http://irep.iium.edu.my/64881/1/64881_The%20expression%20of%20microrna-744.pdf
first_indexed	2023-09-18T21:32:05Z
last_indexed	2023-09-18T21:32:05Z
_version_	1777412578647998464

The expression of microrna-744 (MIR-744) in nasopharyngeal carcinoma: a preliminary study

Similar Items