Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats

Diabetes mellitus (DM) is a chronic disease that can affect metabolism of glucose and other metabolites. In this study, the normal- and obese-diabetic rats were compared to understand the diabetes disorders of type 1 and 2 diabetes mellitus. This was done by evaluating their urine metabolites using...

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Main Authors:	Mediani, Ahmed, Abas, Faridah, M., Maulidiani, Ching, Ping Tan, Khatib, Alfi, Ismail, Intan Safinar, Shaari, Khozirah, Amin, Ismail, Lajis, N.H.
Format:	Article
Language:	English English English
Published:	Springer Netherlands 2018
Subjects:	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/65035/ http://irep.iium.edu.my/65035/ http://irep.iium.edu.my/65035/ http://irep.iium.edu.my/65035/9/65035_Metabolomic%20analysis%20and%20biochemical_SCOPUS.pdf http://irep.iium.edu.my/65035/15/65035_Metabolomic%20analysis%20and%20biochemical.pdf http://irep.iium.edu.my/65035/16/65035_Metabolomic%20analysis%20and%20biochemical_WOS.pdf

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spelling	iium-650352018-10-18T07:34:52Z http://irep.iium.edu.my/65035/ Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats Mediani, Ahmed Abas, Faridah M., Maulidiani Ching, Ping Tan Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Amin, Ismail Lajis, N.H. RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Diabetes mellitus (DM) is a chronic disease that can affect metabolism of glucose and other metabolites. In this study, the normal- and obese-diabetic rats were compared to understand the diabetes disorders of type 1 and 2 diabetes mellitus. This was done by evaluating their urine metabolites using proton nuclear magnetic resonance (1H NMR)-based metabolomics and comparing with controls at different time points, considering the induction periods of obesity and diabetes. The biochemical parameters of the serum were also investigated. The obese-diabetic model was developed by feeding the rats a high-fat diet and inducing diabetic conditions with a low dose of streptozotocin (STZ) (25 mg/kg bw). However, the normal rats were induced by a high dose of STZ (55 mg/kg bw). A partial least squares discriminant analysis (PLS-DA) model showed the biomarkers of both DM types compared to control. The synthesis and degradation of ketone bodies, tricarboxylic (TCA) cycles, and amino acid pathways were the ones most involved in the variation with the highest impact. The diabetic groups also exhibited a noticeable increase in the plasma glucose level and lipid profile disorders compared to the control. There was also an increase in the plasma cholesterol and low-density lipoprotein (LDL) levels and a decline in the high-density lipoprotein (HDL) of diabetic rats. The normal-diabetic rats exhibited the highest effect of all parameters compared to the obese-diabetic rats in the advancement of the DM period. This finding can build a platform to understand the metabolic and biochemical complications of both types of DM and can generate ideas for finding targeted drugs Springer Netherlands 2018-05-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65035/9/65035_Metabolomic%20analysis%20and%20biochemical_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/65035/15/65035_Metabolomic%20analysis%20and%20biochemical.pdf application/pdf en http://irep.iium.edu.my/65035/16/65035_Metabolomic%20analysis%20and%20biochemical_WOS.pdf Mediani, Ahmed and Abas, Faridah and M., Maulidiani and Ching, Ping Tan and Khatib, Alfi and Ismail, Intan Safinar and Shaari, Khozirah and Amin, Ismail and Lajis, N.H. (2018) Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats. Journal of Physiology and Biochemistry, 74 (3). pp. 403-416. ISSN 1138-7548 E-ISSN 1877-8755 (In Press) https://link.springer.com/article/10.1007/s13105-018-0631-3 10.1007/s13105-018-0631-3
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Mediani, Ahmed Abas, Faridah M., Maulidiani Ching, Ping Tan Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Amin, Ismail Lajis, N.H. Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
description	Diabetes mellitus (DM) is a chronic disease that can affect metabolism of glucose and other metabolites. In this study, the normal- and obese-diabetic rats were compared to understand the diabetes disorders of type 1 and 2 diabetes mellitus. This was done by evaluating their urine metabolites using proton nuclear magnetic resonance (1H NMR)-based metabolomics and comparing with controls at different time points, considering the induction periods of obesity and diabetes. The biochemical parameters of the serum were also investigated. The obese-diabetic model was developed by feeding the rats a high-fat diet and inducing diabetic conditions with a low dose of streptozotocin (STZ) (25 mg/kg bw). However, the normal rats were induced by a high dose of STZ (55 mg/kg bw). A partial least squares discriminant analysis (PLS-DA) model showed the biomarkers of both DM types compared to control. The synthesis and degradation of ketone bodies, tricarboxylic (TCA) cycles, and amino acid pathways were the ones most involved in the variation with the highest impact. The diabetic groups also exhibited a noticeable increase in the plasma glucose level and lipid profile disorders compared to the control. There was also an increase in the plasma cholesterol and low-density lipoprotein (LDL) levels and a decline in the high-density lipoprotein (HDL) of diabetic rats. The normal-diabetic rats exhibited the highest effect of all parameters compared to the obese-diabetic rats in the advancement of the DM period. This finding can build a platform to understand the metabolic and biochemical complications of both types of DM and can generate ideas for finding targeted drugs
format	Article
author	Mediani, Ahmed Abas, Faridah M., Maulidiani Ching, Ping Tan Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Amin, Ismail Lajis, N.H.
author_facet	Mediani, Ahmed Abas, Faridah M., Maulidiani Ching, Ping Tan Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Amin, Ismail Lajis, N.H.
author_sort	Mediani, Ahmed
title	Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
title_short	Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
title_full	Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
title_fullStr	Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
title_full_unstemmed	Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
title_sort	metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats
publisher	Springer Netherlands
publishDate	2018
url	http://irep.iium.edu.my/65035/ http://irep.iium.edu.my/65035/ http://irep.iium.edu.my/65035/ http://irep.iium.edu.my/65035/9/65035_Metabolomic%20analysis%20and%20biochemical_SCOPUS.pdf http://irep.iium.edu.my/65035/15/65035_Metabolomic%20analysis%20and%20biochemical.pdf http://irep.iium.edu.my/65035/16/65035_Metabolomic%20analysis%20and%20biochemical_WOS.pdf
first_indexed	2023-09-18T21:32:16Z
last_indexed	2023-09-18T21:32:16Z
_version_	1777412590121517056

Metabolomic analysis and biochemical changes in the urine and serum of streptozotocin-induced normal- and obese-diabetic rats

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