Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms

Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes t...

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Main Authors:	Shahdan, Intan Azura, Mohd Sobri, Fatimah Zahrah, Hanapi, Mohammad Faiz Hizzuan, Ahmad Yusof @ Hanafi, Hanani, How, Fiona Ni Foong
Format:	Article
Language:	English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf

id	iium-65153
recordtype	eprints
spelling	iium-651532018-08-17T01:19:19Z http://irep.iium.edu.my/65153/ Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong QD Chemistry Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of chronic infections including hospital acquired infections that leads to high morbidity and mortality. One of the reasons for the occurrence of resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms against a series of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against the biofilms was conducted using broth microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II) complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25 to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these metal-antibiotics are also proposed. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2016-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf Shahdan, Intan Azura and Mohd Sobri, Fatimah Zahrah and Hanapi, Mohammad Faiz Hizzuan and Ahmad Yusof @ Hanafi, Hanani and How, Fiona Ni Foong (2016) Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms. International Medical Journal Malaysia, 15 (Supplement Issue). p. 9. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-9.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
description	Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of chronic infections including hospital acquired infections that leads to high morbidity and mortality. One of the reasons for the occurrence of resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms against a series of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against the biofilms was conducted using broth microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II) complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25 to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these metal-antibiotics are also proposed.
format	Article
author	Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong
author_facet	Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong
author_sort	Shahdan, Intan Azura
title	Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
title_short	Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
title_full	Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
title_fullStr	Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
title_full_unstemmed	Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
title_sort	antibiotic susceptibility study of metal-gentamicin complexes against pseudomonas aeruginosa biofilms
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf
first_indexed	2023-09-18T21:32:26Z
last_indexed	2023-09-18T21:32:26Z
_version_	1777412600224546816

Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms

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