Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique

Microfluidics-based biochips play a vital role in single-cell research applications. Handling and positioning of single cells at the microscale level are an essential need for various applications, including genomics, proteomics, secretomics, and lysis-analysis. In this article, the pipette Petri...

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Main Authors:	Narayanamurthy, Vigneswaran, Lee, Tze Pin, Firus Khan, Al’aina Yuhainis, Samsuri, Fahmi, Mohamed, Khairudin, Hamzah, Hairul Aini, Baharom, Madia Baizura
Format:	Article
Language:	English English
Published:	MDPI 2018
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/65170/ http://irep.iium.edu.my/65170/ http://irep.iium.edu.my/65170/ http://irep.iium.edu.my/65170/1/65170_Pipette%20Petri%20Dish%20Single-Cell%20Trapping.pdf http://irep.iium.edu.my/65170/7/65170_Pipette%20Petri%20Dish%20Single-Cell%20Trapping_WOS.pdf

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spelling	iium-651702019-01-30T03:27:55Z http://irep.iium.edu.my/65170/ Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique Narayanamurthy, Vigneswaran Lee, Tze Pin Firus Khan, Al’aina Yuhainis Samsuri, Fahmi Mohamed, Khairudin Hamzah, Hairul Aini Baharom, Madia Baizura TA164 Bioengineering Microfluidics-based biochips play a vital role in single-cell research applications. Handling and positioning of single cells at the microscale level are an essential need for various applications, including genomics, proteomics, secretomics, and lysis-analysis. In this article, the pipette Petri dish single-cell trapping (PP-SCT) technique is demonstrated. PP-SCT is a simple and cost-effective technique with ease of implementation for single cell analysis applications. In this paper a wide operation at different fluid flow rates of the novel PP-SCT technique is demonstrated. The effects of the microfluidic channel shape (straight, branched, and serpent) on the efficiency of single-cell trapping are studied. This article exhibited passive microfluidic-based biochips capable of vertical cell trapping with the hexagonally-positioned array of microwells. Microwells were 35 µm in diameter, a size sufficient to allow the attachment of captured cells for short-term study. Single-cell capture (SCC) capabilities of the microfluidic-biochips were found to be improving from the straight channel, branched channel, and serpent channel, accordingly. Multiple cell capture (MCC) was on the order of decreasing from the straight channel, branch channel, and serpent channel. Among the three designs investigated, the serpent channel biochip offers high SCC percentage with reduced MCC and NC (no capture) percentage. SCC was around 52%, 42%, and 35% for the serpent, branched, and straight channel biochips, respectively, for the tilt angle, θ values were between 10–15◦ . Human lung cancer cells (A549) were used for characterization. Using the PP-SCT technique, flow rate variations can be precisely achieved with a flow velocity range of 0.25–4 m/s (fluid channel of 2 mm width and 100 µm height). The upper dish (UD) can be used for low flow rate applications and the lower dish (LD) for high flow rate applications. Passive single-cell analysis applications will be facilitated using this method. MDPI 2018-07-24 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65170/1/65170_Pipette%20Petri%20Dish%20Single-Cell%20Trapping.pdf application/pdf en http://irep.iium.edu.my/65170/7/65170_Pipette%20Petri%20Dish%20Single-Cell%20Trapping_WOS.pdf Narayanamurthy, Vigneswaran and Lee, Tze Pin and Firus Khan, Al’aina Yuhainis and Samsuri, Fahmi and Mohamed, Khairudin and Hamzah, Hairul Aini and Baharom, Madia Baizura (2018) Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique. Fluids, 3 (3). pp. 1-16. ISSN 2311-5521 http://www.mdpi.com/2311-5521/3/3/51 51
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Narayanamurthy, Vigneswaran Lee, Tze Pin Firus Khan, Al’aina Yuhainis Samsuri, Fahmi Mohamed, Khairudin Hamzah, Hairul Aini Baharom, Madia Baizura Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique
description	Microfluidics-based biochips play a vital role in single-cell research applications. Handling and positioning of single cells at the microscale level are an essential need for various applications, including genomics, proteomics, secretomics, and lysis-analysis. In this article, the pipette Petri dish single-cell trapping (PP-SCT) technique is demonstrated. PP-SCT is a simple and cost-effective technique with ease of implementation for single cell analysis applications. In this paper a wide operation at different fluid flow rates of the novel PP-SCT technique is demonstrated. The effects of the microfluidic channel shape (straight, branched, and serpent) on the efficiency of single-cell trapping are studied. This article exhibited passive microfluidic-based biochips capable of vertical cell trapping with the hexagonally-positioned array of microwells. Microwells were 35 µm in diameter, a size sufficient to allow the attachment of captured cells for short-term study. Single-cell capture (SCC) capabilities of the microfluidic-biochips were found to be improving from the straight channel, branched channel, and serpent channel, accordingly. Multiple cell capture (MCC) was on the order of decreasing from the straight channel, branch channel, and serpent channel. Among the three designs investigated, the serpent channel biochip offers high SCC percentage with reduced MCC and NC (no capture) percentage. SCC was around 52%, 42%, and 35% for the serpent, branched, and straight channel biochips, respectively, for the tilt angle, θ values were between 10–15◦ . Human lung cancer cells (A549) were used for characterization. Using the PP-SCT technique, flow rate variations can be precisely achieved with a flow velocity range of 0.25–4 m/s (fluid channel of 2 mm width and 100 µm height). The upper dish (UD) can be used for low flow rate applications and the lower dish (LD) for high flow rate applications. Passive single-cell analysis applications will be facilitated using this method.
format	Article
author	Narayanamurthy, Vigneswaran Lee, Tze Pin Firus Khan, Al’aina Yuhainis Samsuri, Fahmi Mohamed, Khairudin Hamzah, Hairul Aini Baharom, Madia Baizura
author_facet	Narayanamurthy, Vigneswaran Lee, Tze Pin Firus Khan, Al’aina Yuhainis Samsuri, Fahmi Mohamed, Khairudin Hamzah, Hairul Aini Baharom, Madia Baizura
author_sort	Narayanamurthy, Vigneswaran
title	Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique
title_short	Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique
title_full	Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique
title_fullStr	Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique
title_full_unstemmed	Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique
title_sort	pipette petri dish single-cell trapping (pp-sct) in microfluidic platforms: a passive hydrodynamic technique
publisher	MDPI
publishDate	2018
url	http://irep.iium.edu.my/65170/ http://irep.iium.edu.my/65170/ http://irep.iium.edu.my/65170/ http://irep.iium.edu.my/65170/1/65170_Pipette%20Petri%20Dish%20Single-Cell%20Trapping.pdf http://irep.iium.edu.my/65170/7/65170_Pipette%20Petri%20Dish%20Single-Cell%20Trapping_WOS.pdf
first_indexed	2023-09-18T21:32:27Z
last_indexed	2023-09-18T21:32:27Z
_version_	1777412601567772672

Pipette Petri Dish Single-Cell Trapping (PP-SCT) in microfluidic platforms: a passive hydrodynamic technique

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