Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65519/ http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf |
| Summary: | In this paper, the potency of stem and leave extracts of Leea indica locally
known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been
studied. The antioxidant properties of three types of extracts was quantitatively
assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical scavenging activity. The
isolation of antioxidative compounds was conducted through preparative thin layer
chromatography with the use of certain binary solvents. The structure determination
was carried out using Gas Chromatography Mass Spectrometer (GCMS). The
methanol extracts from both stem and leaves exhibited highest antioxidant activity
with lowest IC50 of 24 ug/ml and 81 ug/ml respectively compared to other extracts.
The stem showed stronger antioxidant activity compared to leaves part. Based on
GCMS analysis, the antioxidative compounds isolated from leaves part were
proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one
(1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone (2), Methyl
stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and 9-Oxononanoic
acid (6). The antioxidative compounds isolated from stem part were determined as 1-
(hydroxymethyl)-1,2-ethanediyl ester (7), 9-octadecene (8) and oleic acid (9). This
finding can be used as scientific baseline information for the future treatment of
diseases caused by harmful free radicals. |
|---|