Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species

In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical...

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Main Authors:	Harun, Aiza, Daud, Shaari, Harith, Siti Suhaila, Ab Rahim, Nor Erlena Asmira, Abd Jalil, Mohd Azfar, Muhammad Rosdi, Nurul ‘Atiqah, , Fatin Azieyati, , Naila Nabihah, Mat So'ad, Siti Zaiton, Mohd Hassan, Norazian
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/65519/ http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf

id	iium-65519
recordtype	eprints
spelling	iium-655192018-09-18T06:28:11Z http://irep.iium.edu.my/65519/ Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian QD Chemistry In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical scavenging activity. The isolation of antioxidative compounds was conducted through preparative thin layer chromatography with the use of certain binary solvents. The structure determination was carried out using Gas Chromatography Mass Spectrometer (GCMS). The methanol extracts from both stem and leaves exhibited highest antioxidant activity with lowest IC50 of 24 ug/ml and 81 ug/ml respectively compared to other extracts. The stem showed stronger antioxidant activity compared to leaves part. Based on GCMS analysis, the antioxidative compounds isolated from leaves part were proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone (2), Methyl stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and 9-Oxononanoic acid (6). The antioxidative compounds isolated from stem part were determined as 1- (hydroxymethyl)-1,2-ethanediyl ester (7), 9-octadecene (8) and oleic acid (9). This finding can be used as scientific baseline information for the future treatment of diseases caused by harmful free radicals. 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf Harun, Aiza and Daud, Shaari and Harith, Siti Suhaila and Ab Rahim, Nor Erlena Asmira and Abd Jalil, Mohd Azfar and Muhammad Rosdi, Nurul ‘Atiqah and , Fatin Azieyati and , Naila Nabihah and Mat So'ad, Siti Zaiton and Mohd Hassan, Norazian (2016) Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species. In: Regional Conference On Science, Technology & Social Sciences 2016 (RCSTSS), 4th - 6th December 2016, Cameron Highlands, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
description	In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical scavenging activity. The isolation of antioxidative compounds was conducted through preparative thin layer chromatography with the use of certain binary solvents. The structure determination was carried out using Gas Chromatography Mass Spectrometer (GCMS). The methanol extracts from both stem and leaves exhibited highest antioxidant activity with lowest IC50 of 24 ug/ml and 81 ug/ml respectively compared to other extracts. The stem showed stronger antioxidant activity compared to leaves part. Based on GCMS analysis, the antioxidative compounds isolated from leaves part were proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone (2), Methyl stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and 9-Oxononanoic acid (6). The antioxidative compounds isolated from stem part were determined as 1- (hydroxymethyl)-1,2-ethanediyl ester (7), 9-octadecene (8) and oleic acid (9). This finding can be used as scientific baseline information for the future treatment of diseases caused by harmful free radicals.
format	Conference or Workshop Item
author	Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian
author_facet	Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian
author_sort	Harun, Aiza
title	Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
title_short	Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
title_full	Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
title_fullStr	Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
title_full_unstemmed	Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
title_sort	radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of leea indica species
publishDate	2016
url	http://irep.iium.edu.my/65519/ http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf
first_indexed	2023-09-18T21:32:58Z
last_indexed	2023-09-18T21:32:58Z
_version_	1777412633379471360

Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species

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