Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells

Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells

Lung cancer is the most common type of cancer which the mortality rate increases year by year. Therapeutic drugs could not control the progression of cancer and it contributes to the side effects in normal cells. Thus, an alternative strategy using natural product becomes a focus today. Punica grana...

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Main Authors: Abdul Ghani, Radiah, Nik Abdul Malek, Nik Nurasyikin, Mohd. Ashari, Noor Suryani, Abdullah, Nor Zamzila
Format: Article
Language:English
Published: Science Publishing Corporation 2018
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/65591/
http://irep.iium.edu.my/65591/
http://irep.iium.edu.my/65591/1/PAPER%20SYIKIN%20IJET%202018.pdf
id iium-65591
recordtype eprints
spelling iium-655912018-09-04T09:40:12Z http://irep.iium.edu.my/65591/ Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells Abdul Ghani, Radiah Nik Abdul Malek, Nik Nurasyikin Mohd. Ashari, Noor Suryani Abdullah, Nor Zamzila RB Pathology Lung cancer is the most common type of cancer which the mortality rate increases year by year. Therapeutic drugs could not control the progression of cancer and it contributes to the side effects in normal cells. Thus, an alternative strategy using natural product becomes a focus today. Punica granatum, known as pomegranate has demonstrated the anti-proliferative effect in A549 cells. To further confirm its efficacy, this study aimed to investigate the type of cell death and its pathway in A549 cells. Propium Iodide staining was applied to de-termine the cell cycle profile changes induced by this juice. The determination of type of cell death was done using Annex in-V staining and later will be analyzed using flow cytometer. The pathway to apoptosis was investigated by determining the caspase- 3, 8 and 9 activi-ties. The findings were supported by mitochondrial membrane permeability assay and cytochrome c release detection which were later analyzed using flow cytometer. This study revealed that pomegranate juice induced cell cycle arrest at G0/G1 phase and apoptosis through intrinsic pathway following 24 h treatment. Pomegranate juice caused loss of mitochondrial membrane permeability after 48 h (p<0.05) exposure and a release of cytochrome c in cytosol after 24 h (p<0.05) and 48 h (p<0.01) exposure in treated A549 cells. In caspases analysis, it was showed that there was activation of caspase-3 following 72 h (p<0.01) treatment and caspase-9 after 48 (p<0.01) and 72 h (p<0.05) exposure in treated A549 cells. It can be concluded that pomegranate juice able to cause A549 cell growth inhibition by inducing cell cycle arrest and apoptosis through mitochondrial pathway. Science Publishing Corporation 2018-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65591/1/PAPER%20SYIKIN%20IJET%202018.pdf Abdul Ghani, Radiah and Nik Abdul Malek, Nik Nurasyikin and Mohd. Ashari, Noor Suryani and Abdullah, Nor Zamzila (2018) Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells. International Journal of Engineering & Technology, 7 (3.21). pp. 287-292. ISSN 2227-524X https://www.sciencepubco.com/index.php/ijet/pages/view/Indexing
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RB Pathology
spellingShingle RB Pathology
Abdul Ghani, Radiah
Nik Abdul Malek, Nik Nurasyikin
Mohd. Ashari, Noor Suryani
Abdullah, Nor Zamzila
Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
description Lung cancer is the most common type of cancer which the mortality rate increases year by year. Therapeutic drugs could not control the progression of cancer and it contributes to the side effects in normal cells. Thus, an alternative strategy using natural product becomes a focus today. Punica granatum, known as pomegranate has demonstrated the anti-proliferative effect in A549 cells. To further confirm its efficacy, this study aimed to investigate the type of cell death and its pathway in A549 cells. Propium Iodide staining was applied to de-termine the cell cycle profile changes induced by this juice. The determination of type of cell death was done using Annex in-V staining and later will be analyzed using flow cytometer. The pathway to apoptosis was investigated by determining the caspase- 3, 8 and 9 activi-ties. The findings were supported by mitochondrial membrane permeability assay and cytochrome c release detection which were later analyzed using flow cytometer. This study revealed that pomegranate juice induced cell cycle arrest at G0/G1 phase and apoptosis through intrinsic pathway following 24 h treatment. Pomegranate juice caused loss of mitochondrial membrane permeability after 48 h (p<0.05) exposure and a release of cytochrome c in cytosol after 24 h (p<0.05) and 48 h (p<0.01) exposure in treated A549 cells. In caspases analysis, it was showed that there was activation of caspase-3 following 72 h (p<0.01) treatment and caspase-9 after 48 (p<0.01) and 72 h (p<0.05) exposure in treated A549 cells. It can be concluded that pomegranate juice able to cause A549 cell growth inhibition by inducing cell cycle arrest and apoptosis through mitochondrial pathway.
format Article
author Abdul Ghani, Radiah
Nik Abdul Malek, Nik Nurasyikin
Mohd. Ashari, Noor Suryani
Abdullah, Nor Zamzila
author_facet Abdul Ghani, Radiah
Nik Abdul Malek, Nik Nurasyikin
Mohd. Ashari, Noor Suryani
Abdullah, Nor Zamzila
author_sort Abdul Ghani, Radiah
title Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
title_short Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
title_full Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
title_fullStr Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
title_full_unstemmed Pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
title_sort pomegranate juice induced cell cycle arrest and apoptosis through mitochondria pathway in human lung adenocarcinoma cells
publisher Science Publishing Corporation
publishDate 2018
url http://irep.iium.edu.my/65591/
http://irep.iium.edu.my/65591/
http://irep.iium.edu.my/65591/1/PAPER%20SYIKIN%20IJET%202018.pdf
first_indexed 2023-09-18T21:33:04Z
last_indexed 2023-09-18T21:33:04Z
_version_ 1777412639664635904

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