Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis

This study is on Gum Arabic (GA), which is a plant exudate from Hashab (Acacia senegal) tree. It provides a rich source of non-viscous soluble fiber that is used traditionally in folk medicine. The challenge and aim of this research are the selecting appropriate solvent for extraction of antioxidant...

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Main Authors:	Mohamed Elnour, Ahmed Adam, Mirghani, Mohamed Elwathig Saeed, Kabbashi, Nassereldeen Ahmed, Alam, Md. Zahangir, Musa, Khalid Hamid
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	T Technology (General) TP248.13 Biotechnology TS155 Production management
Online Access:	http://irep.iium.edu.my/65795/ http://irep.iium.edu.my/65795/ http://irep.iium.edu.my/65795/1/65795_Gum%20Arabic%20Determination%20of%20Solvent.pdf

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recordtype	eprints
spelling	iium-657952018-09-21T08:58:17Z http://irep.iium.edu.my/65795/ Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis Mohamed Elnour, Ahmed Adam Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Musa, Khalid Hamid T Technology (General) TP248.13 Biotechnology TS155 Production management This study is on Gum Arabic (GA), which is a plant exudate from Hashab (Acacia senegal) tree. It provides a rich source of non-viscous soluble fiber that is used traditionally in folk medicine. The challenge and aim of this research are the selecting appropriate solvent for extraction of antioxidants from Hashab Gum Arabic (HGA). Solid- liquid solvents extraction (SLSE) is one of the most effective techniques for the recovery of antioxidant and phenolic compounds from HGA. This study investigates the effect of the solvent on the extraction of total phenolic content (TPC) and antioxidant activity from HGA using pure solvents (distilled water, methanol, ethanol and acetone) and their aqueous mixtures at 50% and 100% respectively, within two different locations clay and lateritic soils. The capacity of the extracts was determined by the scavenging activity of 1,1-diphenyl-2- picrylhydrazyl (DPPH+•) radical, ferric reducing antioxidant power (FRAP) assay, and Folin-Ciocalteu Index (FCI) assay for TPC. The results indicated that the 100% methanol was more efficient solvent in the extracting of phenolic compounds, and get the highest antioxidant capacities. Moreover, HGA presented the highest antioxidant capacities and total phenolic content compared to other samples from Acacia spp. The coefficient of determination (R2) indicated that there is a significant (P≤0.05) correlation between TPC and FRAP, and CUPRAC respectively, however, there is no correlation between TPC and DPPH in terms of different location clay and lateritic soil. HGA extract is rich in polyphenolic compounds which are extremely affected by the solvent combinations used. In addition, the obtained extracts using pure methanol was distinguished from the extracts obtained with mixtures of organic solvents, through the multivariate analysis using PCA and HCA analyses. Based on these findings, it is concluded that Acacia senegal gum (HGA) constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products. 2018-08-01 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/65795/1/65795_Gum%20Arabic%20Determination%20of%20Solvent.pdf Mohamed Elnour, Ahmed Adam and Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed and Alam, Md. Zahangir and Musa, Khalid Hamid (2018) Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis. In: 4th International Conference on Chemical Engineering and Industrial Biotechnology (ICCEIB 2018), 1st-2nd August 2018, Kuantan, Pahang. (Unpublished) http://icceib.ump.edu.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General) TP248.13 Biotechnology TS155 Production management
spellingShingle	T Technology (General) TP248.13 Biotechnology TS155 Production management Mohamed Elnour, Ahmed Adam Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Musa, Khalid Hamid Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
description	This study is on Gum Arabic (GA), which is a plant exudate from Hashab (Acacia senegal) tree. It provides a rich source of non-viscous soluble fiber that is used traditionally in folk medicine. The challenge and aim of this research are the selecting appropriate solvent for extraction of antioxidants from Hashab Gum Arabic (HGA). Solid- liquid solvents extraction (SLSE) is one of the most effective techniques for the recovery of antioxidant and phenolic compounds from HGA. This study investigates the effect of the solvent on the extraction of total phenolic content (TPC) and antioxidant activity from HGA using pure solvents (distilled water, methanol, ethanol and acetone) and their aqueous mixtures at 50% and 100% respectively, within two different locations clay and lateritic soils. The capacity of the extracts was determined by the scavenging activity of 1,1-diphenyl-2- picrylhydrazyl (DPPH+•) radical, ferric reducing antioxidant power (FRAP) assay, and Folin-Ciocalteu Index (FCI) assay for TPC. The results indicated that the 100% methanol was more efficient solvent in the extracting of phenolic compounds, and get the highest antioxidant capacities. Moreover, HGA presented the highest antioxidant capacities and total phenolic content compared to other samples from Acacia spp. The coefficient of determination (R2) indicated that there is a significant (P≤0.05) correlation between TPC and FRAP, and CUPRAC respectively, however, there is no correlation between TPC and DPPH in terms of different location clay and lateritic soil. HGA extract is rich in polyphenolic compounds which are extremely affected by the solvent combinations used. In addition, the obtained extracts using pure methanol was distinguished from the extracts obtained with mixtures of organic solvents, through the multivariate analysis using PCA and HCA analyses. Based on these findings, it is concluded that Acacia senegal gum (HGA) constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products.
format	Conference or Workshop Item
author	Mohamed Elnour, Ahmed Adam Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Musa, Khalid Hamid
author_facet	Mohamed Elnour, Ahmed Adam Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Musa, Khalid Hamid
author_sort	Mohamed Elnour, Ahmed Adam
title	Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
title_short	Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
title_full	Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
title_fullStr	Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
title_full_unstemmed	Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
title_sort	gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis
publishDate	2018
url	http://irep.iium.edu.my/65795/ http://irep.iium.edu.my/65795/ http://irep.iium.edu.my/65795/1/65795_Gum%20Arabic%20Determination%20of%20Solvent.pdf
first_indexed	2023-09-18T21:33:20Z
last_indexed	2023-09-18T21:33:20Z
_version_	1777412656921051136

Gum arabic: determination of solvent effect on the extraction of phenolic compounds and antioxidant activity using principle component analysis

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