Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan

Background: There had been an increase in the awareness of microbial contamination in the world today. It can often go unnoticed or be found in the most unexpected places. With the increase in infectious disease globally, it is more important for us to take preventive measures in order to prevent f...

Full description

Bibliographic Details
Main Authors:	Yusoff, Iylia Nurliyana, Alias, Norsyuhada
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/65831/ http://irep.iium.edu.my/65831/ http://irep.iium.edu.my/65831/1/65831_Isolation%20of%20bacterial%20contaminants-%20tentative.pdf

id	iium-65831
recordtype	eprints
spelling	iium-658312018-09-24T08:50:12Z http://irep.iium.edu.my/65831/ Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan Yusoff, Iylia Nurliyana Alias, Norsyuhada QR Microbiology Background: There had been an increase in the awareness of microbial contamination in the world today. It can often go unnoticed or be found in the most unexpected places. With the increase in infectious disease globally, it is more important for us to take preventive measures in order to prevent further spreading of bacterial contamination. In this study, we have identified and measured the bacterial contamination that can be found on the surface of elevator buttons of Kuliyyah of Medicine and Office of Campus Director in International Islamic University Malaysia Kuantan Campus (IIUMK). Methods: The study was a cross-sectional research. Samples were taken from elevator buttons from the surface of elevator buttons of Kuliyyah of Medicine and Office of Campus Director in IIUMK using sterile cotton swab moistened with nutrient broth. Enumeration of bacteria was done using the viable plate count method. The enumeration of bacterial contaminants was recorded as CFU/ml and classified into low, medium or high contamination level. Morphology characteristic of bacterial contaminants was observed by streaking bacterial colonies on mannitol salt agar and MacConkey agar according to the standard microbiological methods. Finally, Gram staining and PCR amplification of 16rRNA were carried out to further distinguished and identified the type of bacteria. Results: This study was conducted on 89 buttons from five elevators available in Kuliyyah of Medicine and Office of Campus Director in IIUMK. Eighty-four percent of the samples showed growth of bacterial contamination. Enumeration of bacteria was categorised into low, medium and high contamination categories. Fifty elevator buttons were categorised having low contamination and twelve elevator buttons are classified as having medium contamination. Twelve elevator buttons is categorised into high contamination category. It is expected that the elevator buttons were dominated by Staphylococcus and Enterobacter species based on morphological characteristics and Gram types. Molecular identification via PCR is still underway. Conclusions: Preliminary result revealed there is a presence of bacterial contaminants on the surface of the elevator buttons of Kuliyyah of Medicine and Office of Campus Director in IIUMK. Hence, elevator buttons could be the transmitting medium of pathogenic bacteria. The findings signify the importance of routine elevator buttons disinfection to lessen bacterial transmission. 2018-05-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/65831/1/65831_Isolation%20of%20bacterial%20contaminants-%20tentative.pdf Yusoff, Iylia Nurliyana and Alias, Norsyuhada (2018) Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan. In: 9th Malaysian Symposium of Biomedical Science, 12th-13rd May 2018, Balai Ungku Aziz, Faculty of Dentistry, University of Malaya. (Unpublished) https://9thmysymbiosbios.wixsite.com/myum
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Yusoff, Iylia Nurliyana Alias, Norsyuhada Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan
description	Background: There had been an increase in the awareness of microbial contamination in the world today. It can often go unnoticed or be found in the most unexpected places. With the increase in infectious disease globally, it is more important for us to take preventive measures in order to prevent further spreading of bacterial contamination. In this study, we have identified and measured the bacterial contamination that can be found on the surface of elevator buttons of Kuliyyah of Medicine and Office of Campus Director in International Islamic University Malaysia Kuantan Campus (IIUMK). Methods: The study was a cross-sectional research. Samples were taken from elevator buttons from the surface of elevator buttons of Kuliyyah of Medicine and Office of Campus Director in IIUMK using sterile cotton swab moistened with nutrient broth. Enumeration of bacteria was done using the viable plate count method. The enumeration of bacterial contaminants was recorded as CFU/ml and classified into low, medium or high contamination level. Morphology characteristic of bacterial contaminants was observed by streaking bacterial colonies on mannitol salt agar and MacConkey agar according to the standard microbiological methods. Finally, Gram staining and PCR amplification of 16rRNA were carried out to further distinguished and identified the type of bacteria. Results: This study was conducted on 89 buttons from five elevators available in Kuliyyah of Medicine and Office of Campus Director in IIUMK. Eighty-four percent of the samples showed growth of bacterial contamination. Enumeration of bacteria was categorised into low, medium and high contamination categories. Fifty elevator buttons were categorised having low contamination and twelve elevator buttons are classified as having medium contamination. Twelve elevator buttons is categorised into high contamination category. It is expected that the elevator buttons were dominated by Staphylococcus and Enterobacter species based on morphological characteristics and Gram types. Molecular identification via PCR is still underway. Conclusions: Preliminary result revealed there is a presence of bacterial contaminants on the surface of the elevator buttons of Kuliyyah of Medicine and Office of Campus Director in IIUMK. Hence, elevator buttons could be the transmitting medium of pathogenic bacteria. The findings signify the importance of routine elevator buttons disinfection to lessen bacterial transmission.
format	Conference or Workshop Item
author	Yusoff, Iylia Nurliyana Alias, Norsyuhada
author_facet	Yusoff, Iylia Nurliyana Alias, Norsyuhada
author_sort	Yusoff, Iylia Nurliyana
title	Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan
title_short	Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan
title_full	Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan
title_fullStr	Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan
title_full_unstemmed	Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan
title_sort	isolation of bacterial contaminants from elevator buttons of kulliyyah of medicine and office of campus director in international islamic university malaysia kuantan
publishDate	2018
url	http://irep.iium.edu.my/65831/ http://irep.iium.edu.my/65831/ http://irep.iium.edu.my/65831/1/65831_Isolation%20of%20bacterial%20contaminants-%20tentative.pdf
first_indexed	2023-09-18T21:33:23Z
last_indexed	2023-09-18T21:33:23Z
_version_	1777412660537589760

Isolation of bacterial contaminants from elevator buttons of Kulliyyah of Medicine and office of Campus Director in International Islamic University Malaysia Kuantan

Similar Items