Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression

Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression

Introduction: Acute lymphoblastic leukemia (ALL) is the most common leukemia subtypes among paediatrics in Malaysia. Although treatment options are available but some patients remain incurable, some undergo relapse and many experiences adverse effects by the conventional therapies. Thus, we aim to i...

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Main Authors: Zainudin, Mohammad Fauzan, Fadzir, Ummu Afifah, Rosdi, Athirah, Johan, Muhammad Farid, Hashim, Ridzwan, Abdul Wahab, Ridhwan, Mamat, Suhana
Format: Article
Language:English
Published: Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/66043/
http://irep.iium.edu.my/66043/
http://irep.iium.edu.my/66043/1/fauzan%20et%20al.%202018.pdf
id iium-66043
recordtype eprints
spelling iium-660432018-10-02T01:47:07Z http://irep.iium.edu.my/66043/ Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression Zainudin, Mohammad Fauzan Fadzir, Ummu Afifah Rosdi, Athirah Johan, Muhammad Farid Hashim, Ridzwan Abdul Wahab, Ridhwan Mamat, Suhana RB Pathology Introduction: Acute lymphoblastic leukemia (ALL) is the most common leukemia subtypes among paediatrics in Malaysia. Although treatment options are available but some patients remain incurable, some undergo relapse and many experiences adverse effects by the conventional therapies. Thus, we aim to investigate possible treatment alternative by studying the antileukemogenesis properties of concentrated Nypa fruticans sap called nisaan by focusing on adiponectin expression. Method: Our study model was CCRF-CEM, an acute lymphoblastic leukemia cell lines. The cells were treated with nisaan at a range of concentration and treated for 24, 48 and 72 hours followed by determination of the leukemic cells viability using tryphan blue method. Effective nisaan concentrations that significantly reduced the cells viability were again treated to the cells followed by determination of the cell proliferation using BrdU colorimetric kit and adiponectin level using adiponectin ELISA kit. Results: The results showed that, increase concentration of nisaan treatment reduced the cells viability and cells proliferation and enhance the adiponectin level in the leukemic cells. Conclusion: This preliminary data suggest that Nypa fruticans might has the anti-leukemogenesis effect on acute lymphoblastic cells by regulating the adiponectin expression. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017-07-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66043/1/fauzan%20et%20al.%202018.pdf Zainudin, Mohammad Fauzan and Fadzir, Ummu Afifah and Rosdi, Athirah and Johan, Muhammad Farid and Hashim, Ridzwan and Abdul Wahab, Ridhwan and Mamat, Suhana (2017) Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression. International Journal of Allied Health Sciences, 1 (2). pp. 28-43. E-ISSN 2600-8491 http://journals.iium.edu.my/ijahs/index.php/IJAHS/issue/view/3
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RB Pathology
spellingShingle RB Pathology
Zainudin, Mohammad Fauzan
Fadzir, Ummu Afifah
Rosdi, Athirah
Johan, Muhammad Farid
Hashim, Ridzwan
Abdul Wahab, Ridhwan
Mamat, Suhana
Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
description Introduction: Acute lymphoblastic leukemia (ALL) is the most common leukemia subtypes among paediatrics in Malaysia. Although treatment options are available but some patients remain incurable, some undergo relapse and many experiences adverse effects by the conventional therapies. Thus, we aim to investigate possible treatment alternative by studying the antileukemogenesis properties of concentrated Nypa fruticans sap called nisaan by focusing on adiponectin expression. Method: Our study model was CCRF-CEM, an acute lymphoblastic leukemia cell lines. The cells were treated with nisaan at a range of concentration and treated for 24, 48 and 72 hours followed by determination of the leukemic cells viability using tryphan blue method. Effective nisaan concentrations that significantly reduced the cells viability were again treated to the cells followed by determination of the cell proliferation using BrdU colorimetric kit and adiponectin level using adiponectin ELISA kit. Results: The results showed that, increase concentration of nisaan treatment reduced the cells viability and cells proliferation and enhance the adiponectin level in the leukemic cells. Conclusion: This preliminary data suggest that Nypa fruticans might has the anti-leukemogenesis effect on acute lymphoblastic cells by regulating the adiponectin expression.
format Article
author Zainudin, Mohammad Fauzan
Fadzir, Ummu Afifah
Rosdi, Athirah
Johan, Muhammad Farid
Hashim, Ridzwan
Abdul Wahab, Ridhwan
Mamat, Suhana
author_facet Zainudin, Mohammad Fauzan
Fadzir, Ummu Afifah
Rosdi, Athirah
Johan, Muhammad Farid
Hashim, Ridzwan
Abdul Wahab, Ridhwan
Mamat, Suhana
author_sort Zainudin, Mohammad Fauzan
title Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
title_short Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
title_full Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
title_fullStr Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
title_full_unstemmed Cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
title_sort cytotoxicity of leukemic cells by nypa fruticans through regulation of adiponectin expression
publisher Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
publishDate 2017
url http://irep.iium.edu.my/66043/
http://irep.iium.edu.my/66043/
http://irep.iium.edu.my/66043/1/fauzan%20et%20al.%202018.pdf
first_indexed 2023-09-18T21:33:43Z
last_indexed 2023-09-18T21:33:43Z
_version_ 1777412680660811776

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