Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings

Introduction: Lift buttons are among the potential fomites in transmitting infectious microorganism to the community. The daily use of lift buttons by different people with various hygiene practices can contribute to the colonization of microorganism on the touched surfaces. There is few published r...

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Main Authors:	Mohd Danial, Nur Izzatul Husna, Alias, Norsyuhada, Mohd Fauzi, Fadhin Sakina
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/66099/ http://irep.iium.edu.my/66099/1/MRS%202018%20scanned%20abstract.pdf

id	iium-66099
recordtype	eprints
spelling	iium-660992018-10-01T06:23:25Z http://irep.iium.edu.my/66099/ Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings Mohd Danial, Nur Izzatul Husna Alias, Norsyuhada Mohd Fauzi, Fadhin Sakina QR Microbiology Introduction: Lift buttons are among the potential fomites in transmitting infectious microorganism to the community. The daily use of lift buttons by different people with various hygiene practices can contribute to the colonization of microorganism on the touched surfaces. There is few published research on microbial colonization on lift buttons in Malaysia, especially in the campus setting. Hence, this study aimed to identify and compare the bacterial load on lift buttons from a kulliyyah (faculty) and the library at IIUM Kuantan. Materials and Method: A total of 42 swab samples were collected aseptically from the lift buttons and cultured on nutrient, mannitol salt and MacConkey agar for bacterial enumeration and isolation. Gram staining, microscopy examination and molecular amplification of the 16S rDNA gene by polymerase chain reaction were performed for further characterization of the bacteria. Results: Out of 42 swab samples collected (kulliyyah: n =25, library: n=17), 88.4% of the samples confirmed the presence of bacteria with the lift buttons in the kulliyyah showed a higher percentage of bacterial isolation (92%) compared to the library (84%). Gram-positive cocci clustered bacteria dominated the isolates followed by Gram-positive bacilli, Gram-positive cocci tetrads and Gram-negative coccobacilli. Based on culture growth, Gram-staining features and 16S rDNA gene sequencing, isolated bacteria were identified as Staphylococcus sp., Staphylococcus kloosii, Bacillus cereus, Kocuria palustris, Yersinia mollaretii, Bacillus sp., Micrococcus sp., and other Gram-positive cocci. Conclusion: The results revealed a high bacterial load on lift buttons which can be a source of bacterial transmission. 2018-08-29 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/66099/1/MRS%202018%20scanned%20abstract.pdf Mohd Danial, Nur Izzatul Husna and Alias, Norsyuhada and Mohd Fauzi, Fadhin Sakina (2018) Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings. In: Medical Research Symposium 2018, 29th Aug. 2018, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohd Danial, Nur Izzatul Husna Alias, Norsyuhada Mohd Fauzi, Fadhin Sakina Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings
description	Introduction: Lift buttons are among the potential fomites in transmitting infectious microorganism to the community. The daily use of lift buttons by different people with various hygiene practices can contribute to the colonization of microorganism on the touched surfaces. There is few published research on microbial colonization on lift buttons in Malaysia, especially in the campus setting. Hence, this study aimed to identify and compare the bacterial load on lift buttons from a kulliyyah (faculty) and the library at IIUM Kuantan. Materials and Method: A total of 42 swab samples were collected aseptically from the lift buttons and cultured on nutrient, mannitol salt and MacConkey agar for bacterial enumeration and isolation. Gram staining, microscopy examination and molecular amplification of the 16S rDNA gene by polymerase chain reaction were performed for further characterization of the bacteria. Results: Out of 42 swab samples collected (kulliyyah: n =25, library: n=17), 88.4% of the samples confirmed the presence of bacteria with the lift buttons in the kulliyyah showed a higher percentage of bacterial isolation (92%) compared to the library (84%). Gram-positive cocci clustered bacteria dominated the isolates followed by Gram-positive bacilli, Gram-positive cocci tetrads and Gram-negative coccobacilli. Based on culture growth, Gram-staining features and 16S rDNA gene sequencing, isolated bacteria were identified as Staphylococcus sp., Staphylococcus kloosii, Bacillus cereus, Kocuria palustris, Yersinia mollaretii, Bacillus sp., Micrococcus sp., and other Gram-positive cocci. Conclusion: The results revealed a high bacterial load on lift buttons which can be a source of bacterial transmission.
format	Conference or Workshop Item
author	Mohd Danial, Nur Izzatul Husna Alias, Norsyuhada Mohd Fauzi, Fadhin Sakina
author_facet	Mohd Danial, Nur Izzatul Husna Alias, Norsyuhada Mohd Fauzi, Fadhin Sakina
author_sort	Mohd Danial, Nur Izzatul Husna
title	Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings
title_short	Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings
title_full	Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings
title_fullStr	Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings
title_full_unstemmed	Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings
title_sort	comparison of bacterial loads from the lift buttons of a kulliyyah and a library at iium kuantan and identification of the bacteria from selected buildings
publishDate	2018
url	http://irep.iium.edu.my/66099/ http://irep.iium.edu.my/66099/1/MRS%202018%20scanned%20abstract.pdf
first_indexed	2023-09-18T21:33:47Z
last_indexed	2023-09-18T21:33:47Z
_version_	1777412685160251392

Comparison of bacterial loads from the lift buttons of a kulliyyah and a library at IIUM Kuantan and identification of the bacteria from selected buildings

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