Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure

Introduction: There were significant epidemiological evidences that suggested the association of end-stage renal diseases of unknown aetiology with chronic pesticide exposure.Our previous assessment of renal tissues from Sprague-Dawley rats exposed to chronic low dose OP had found significant...

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Main Authors:	Aung, Sanda, Abdullah, Nor Zamzila, A.Talib, Norlelawati, Mat Zainone, Zamzuria, Abdul Razak, Tariq
Format:	Article
Language:	English
Published:	Malaysian Society of Pathologists 2018
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/66480/ http://irep.iium.edu.my/66480/ http://irep.iium.edu.my/66480/1/66480_Oxidative%20stress%20induced.pdf

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spelling	iium-664802018-10-10T01:33:22Z http://irep.iium.edu.my/66480/ Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure Aung, Sanda Abdullah, Nor Zamzila A.Talib, Norlelawati Mat Zainone, Zamzuria Abdul Razak, Tariq RB Pathology Introduction: There were significant epidemiological evidences that suggested the association of end-stage renal diseases of unknown aetiology with chronic pesticide exposure.Our previous assessment of renal tissues from Sprague-Dawley rats exposed to chronic low dose OP had found significant microscopic features consistent with renal tubular damage. This study postulated that the renal damage is the consequences of oxidative stress which may lead to cell death through apoptosis. The aims of the current study is to determine the pathways involved in the above microscopic changes. Materials & Methods: Two groups of males Sprague-Dawley rats of 6 rats each: control group and exposed group (received an alternate day of subcutaneous 18.0 mg/kg BW chlorpyrifos) were sacrificed at 150 days. A small tissue section from the renal cortex was preserved in RNAlater whilst the other half of the renal was preserved in formalin for tissue processing. RNA was purified from the tissue preserved in RNAlater and subsequently subjected to qPCR array on real-time platform. The relative expression of selected markers representing the oxidative stress and apoptosis pathways was normalized to two reference genes and quantitatively measured using ΔΔCq method. Immunohistochemistry for MDA and p53 were performed in formalin fixed paraffin embedded renal tissue. Results: There were significant downregulation of GR (p=0.04), NOS2 (p=0.03) and Ripk3 (p=0.01) and significant upregulation of p53 (p=0.02). Subsequently IHC test showed significant MDA and p53 expression. Discussion: The OP exposure to the kidney results in tubular injury through oxidative pathways and subsequent cell arrest by activation of p53. Malaysian Society of Pathologists 2018-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66480/1/66480_Oxidative%20stress%20induced.pdf Aung, Sanda and Abdullah, Nor Zamzila and A.Talib, Norlelawati and Mat Zainone, Zamzuria and Abdul Razak, Tariq (2018) Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure. The Malaysian Journal of Pathology, 40 (2). pp. 242-243. ISSN 0126-8635 http://www.mjpath.org.my/2018/v40n2/abstracts.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Aung, Sanda Abdullah, Nor Zamzila A.Talib, Norlelawati Mat Zainone, Zamzuria Abdul Razak, Tariq Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure
description	Introduction: There were significant epidemiological evidences that suggested the association of end-stage renal diseases of unknown aetiology with chronic pesticide exposure.Our previous assessment of renal tissues from Sprague-Dawley rats exposed to chronic low dose OP had found significant microscopic features consistent with renal tubular damage. This study postulated that the renal damage is the consequences of oxidative stress which may lead to cell death through apoptosis. The aims of the current study is to determine the pathways involved in the above microscopic changes. Materials & Methods: Two groups of males Sprague-Dawley rats of 6 rats each: control group and exposed group (received an alternate day of subcutaneous 18.0 mg/kg BW chlorpyrifos) were sacrificed at 150 days. A small tissue section from the renal cortex was preserved in RNAlater whilst the other half of the renal was preserved in formalin for tissue processing. RNA was purified from the tissue preserved in RNAlater and subsequently subjected to qPCR array on real-time platform. The relative expression of selected markers representing the oxidative stress and apoptosis pathways was normalized to two reference genes and quantitatively measured using ΔΔCq method. Immunohistochemistry for MDA and p53 were performed in formalin fixed paraffin embedded renal tissue. Results: There were significant downregulation of GR (p=0.04), NOS2 (p=0.03) and Ripk3 (p=0.01) and significant upregulation of p53 (p=0.02). Subsequently IHC test showed significant MDA and p53 expression. Discussion: The OP exposure to the kidney results in tubular injury through oxidative pathways and subsequent cell arrest by activation of p53.
format	Article
author	Aung, Sanda Abdullah, Nor Zamzila A.Talib, Norlelawati Mat Zainone, Zamzuria Abdul Razak, Tariq
author_facet	Aung, Sanda Abdullah, Nor Zamzila A.Talib, Norlelawati Mat Zainone, Zamzuria Abdul Razak, Tariq
author_sort	Aung, Sanda
title	Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure
title_short	Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure
title_full	Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure
title_fullStr	Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure
title_full_unstemmed	Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure
title_sort	oxidative stress induced nephrotoxicity in chronic low dose organophosphates (op) exposure
publisher	Malaysian Society of Pathologists
publishDate	2018
url	http://irep.iium.edu.my/66480/ http://irep.iium.edu.my/66480/ http://irep.iium.edu.my/66480/1/66480_Oxidative%20stress%20induced.pdf
first_indexed	2023-09-18T21:34:23Z
last_indexed	2023-09-18T21:34:23Z
_version_	1777412722706612224

Oxidative stress induced nephrotoxicity in chronic low dose organophosphates (OP) exposure

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