Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)

Friable callus of Citrus suhuiensis (C. suhuiensis) derived from its cotyledon seed was inoculated for cell suspension culture establishment. Face Centred Central Composite Design (FCCCD) and Response Surface Methodology (RSM) in Design Expert 9.0 software were used to optimize C. suhuiensis cell su...

Full description

Bibliographic Details
Main Authors:	M. Fathil, Nur Alia, Mohamad Puad, Noor Illi, Azmi, Azlin Suhaida, Amid, Azura
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2018
Subjects:	SB Plant culture TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/66551/ http://irep.iium.edu.my/66551/ http://irep.iium.edu.my/66551/1/66551_Optimization%20of%20Citrus%20suhuiensis.pdf

id	iium-66551
recordtype	eprints
spelling	iium-665512018-10-12T03:38:38Z http://irep.iium.edu.my/66551/ Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM) M. Fathil, Nur Alia Mohamad Puad, Noor Illi Azmi, Azlin Suhaida Amid, Azura SB Plant culture TP248.13 Biotechnology Friable callus of Citrus suhuiensis (C. suhuiensis) derived from its cotyledon seed was inoculated for cell suspension culture establishment. Face Centred Central Composite Design (FCCCD) and Response Surface Methodology (RSM) in Design Expert 9.0 software were used to optimize C. suhuiensis cell suspension culture conditions. Prior to that, one-factor-at-a-time (OFAT) method was carried out to determine the suitable ranges of three factors for FCCCD which are carbon source, plant growth regulators (PGRs) and inoculum size. The cells were cultivated in Murashige and Skoog (MS) media supplemented with different concentrations of sucrose (40-60 g/L), 0.25-0.75 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 5-15% (v/v) inoculum. Result showed that the maximum cell dry weight was 21.42 g/L when media containing sucrose at 50 g/L, 2,4-D at 0.5 mg/L and 15% of inoculum were used. Based on ANOVA analysis, the model was significant while the p-value showed that the linear terms of 2,4-D and inoculum size as well as the quadratic terms of glucose and 2,4-D were highly significant (p<0.01). Linear and quadratic effects of 2,4-D were significant, indicating that small changes of 2,4-D would alter C. suhuiensis cell density. Kulliyyah of Engineering, International Islamic University Malaysia 2018-09 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/66551/1/66551_Optimization%20of%20Citrus%20suhuiensis.pdf M. Fathil, Nur Alia and Mohamad Puad, Noor Illi and Azmi, Azlin Suhaida and Amid, Azura (2018) Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM). In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur. http://www.iium.edu.my/icbioe/2018/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SB Plant culture TP248.13 Biotechnology
spellingShingle	SB Plant culture TP248.13 Biotechnology M. Fathil, Nur Alia Mohamad Puad, Noor Illi Azmi, Azlin Suhaida Amid, Azura Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)
description	Friable callus of Citrus suhuiensis (C. suhuiensis) derived from its cotyledon seed was inoculated for cell suspension culture establishment. Face Centred Central Composite Design (FCCCD) and Response Surface Methodology (RSM) in Design Expert 9.0 software were used to optimize C. suhuiensis cell suspension culture conditions. Prior to that, one-factor-at-a-time (OFAT) method was carried out to determine the suitable ranges of three factors for FCCCD which are carbon source, plant growth regulators (PGRs) and inoculum size. The cells were cultivated in Murashige and Skoog (MS) media supplemented with different concentrations of sucrose (40-60 g/L), 0.25-0.75 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 5-15% (v/v) inoculum. Result showed that the maximum cell dry weight was 21.42 g/L when media containing sucrose at 50 g/L, 2,4-D at 0.5 mg/L and 15% of inoculum were used. Based on ANOVA analysis, the model was significant while the p-value showed that the linear terms of 2,4-D and inoculum size as well as the quadratic terms of glucose and 2,4-D were highly significant (p<0.01). Linear and quadratic effects of 2,4-D were significant, indicating that small changes of 2,4-D would alter C. suhuiensis cell density.
format	Conference or Workshop Item
author	M. Fathil, Nur Alia Mohamad Puad, Noor Illi Azmi, Azlin Suhaida Amid, Azura
author_facet	M. Fathil, Nur Alia Mohamad Puad, Noor Illi Azmi, Azlin Suhaida Amid, Azura
author_sort	M. Fathil, Nur Alia
title	Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)
title_short	Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)
title_full	Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)
title_fullStr	Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)
title_full_unstemmed	Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)
title_sort	optimization of citrus suhuiensis suspension culture conditions using response surface methodology (rsm)
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/66551/ http://irep.iium.edu.my/66551/ http://irep.iium.edu.my/66551/1/66551_Optimization%20of%20Citrus%20suhuiensis.pdf
first_indexed	2023-09-18T21:34:30Z
last_indexed	2023-09-18T21:34:30Z
_version_	1777412730687324160

Optimization of citrus suhuiensis suspension culture conditions using Response Surface Methodology (RSM)

Similar Items