RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study

Introduction: Runt-related transcription factor 2 (RUNX2) plays important roles in osteoblast differentiation, tooth development and chondrocyte maturation; hence its involvement in craniofacial development is paramount. Mutation in RUNX2 is implicated with cleidocranial dysplasia; a bone developmen...

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Main Authors:	Mokhtar@Makhtar, Khairani Idah, Abu Bakar, Noraini, Kharuddin, Azrul Fazwan
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/66589/ http://irep.iium.edu.my/66589/ http://irep.iium.edu.my/66589/1/ACMBMB2018-Program-Book-3-pages-1%2C4%2C10%2C36%2C97.pdf http://irep.iium.edu.my/66589/2/KIM-ACMBMB%202018%20%282%29.pdf

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recordtype	eprints
spelling	iium-665892018-10-12T04:07:09Z http://irep.iium.edu.my/66589/ RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan RK Dentistry Introduction: Runt-related transcription factor 2 (RUNX2) plays important roles in osteoblast differentiation, tooth development and chondrocyte maturation; hence its involvement in craniofacial development is paramount. Mutation in RUNX2 is implicated with cleidocranial dysplasia; a bone development disorder, while single nucleotide polymorphism (SNP) in RUNX2 is associated with Class II/D2 malocclusion. Although genetic factor has been associated with the incidence of malocclusion; very limited study was conducted to determine the association of certain genes with the incidence of malocclusion in Malaysia. Aim: Thus, this preliminary study aimed to determine the presence and association of RUNX2 SNP (rs6930053) in Class II malocclusion patients. Methods: Genomic DNA was extracted from unstimulated saliva of 31 Class I (control samples) and 30 Class II malocclusion patients. Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for marker rs6930053 and the genotyping was done by sequencing. Chi-square test was used to determine differences in allele and genotype frequencies. Results: Significant difference was detected in allele (p=3.04X10-6) and genotype (p=4.06X10-6) frequencies between control (Class I) and Class II malocclusion. Discussion: This result suggested there is a genetic association between RUNX2 (rs6930053) with Class II malocclusion (p=3.04X10-6, OR= 6.59; 95% CI=2.88~15.08). We provide preliminary observation that RUNX2 SNP (rs6930053) might contribute to Class II malocclusion in our local population. Further studies involving larger number of samples and other DNA markers of RUNX2 gene should be developed in order to understand the exact role and mechanism of RUNX2 in different classes of malocclusions and how this polymorphism affects the malocclusion cases in Malaysian population. 2018 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/66589/1/ACMBMB2018-Program-Book-3-pages-1%2C4%2C10%2C36%2C97.pdf application/pdf en http://irep.iium.edu.my/66589/2/KIM-ACMBMB%202018%20%282%29.pdf Mokhtar@Makhtar, Khairani Idah and Abu Bakar, Noraini and Kharuddin, Azrul Fazwan (2018) RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study. In: 3rd ASEAN Congress on Medical Biotechnology and Molecular Biosciences, 9-10th July 2018, Penang, Malaysia. (Unpublished) https://acmbmb.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study
description	Introduction: Runt-related transcription factor 2 (RUNX2) plays important roles in osteoblast differentiation, tooth development and chondrocyte maturation; hence its involvement in craniofacial development is paramount. Mutation in RUNX2 is implicated with cleidocranial dysplasia; a bone development disorder, while single nucleotide polymorphism (SNP) in RUNX2 is associated with Class II/D2 malocclusion. Although genetic factor has been associated with the incidence of malocclusion; very limited study was conducted to determine the association of certain genes with the incidence of malocclusion in Malaysia. Aim: Thus, this preliminary study aimed to determine the presence and association of RUNX2 SNP (rs6930053) in Class II malocclusion patients. Methods: Genomic DNA was extracted from unstimulated saliva of 31 Class I (control samples) and 30 Class II malocclusion patients. Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for marker rs6930053 and the genotyping was done by sequencing. Chi-square test was used to determine differences in allele and genotype frequencies. Results: Significant difference was detected in allele (p=3.04X10-6) and genotype (p=4.06X10-6) frequencies between control (Class I) and Class II malocclusion. Discussion: This result suggested there is a genetic association between RUNX2 (rs6930053) with Class II malocclusion (p=3.04X10-6, OR= 6.59; 95% CI=2.88~15.08). We provide preliminary observation that RUNX2 SNP (rs6930053) might contribute to Class II malocclusion in our local population. Further studies involving larger number of samples and other DNA markers of RUNX2 gene should be developed in order to understand the exact role and mechanism of RUNX2 in different classes of malocclusions and how this polymorphism affects the malocclusion cases in Malaysian population.
format	Conference or Workshop Item
author	Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan
author_facet	Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan
author_sort	Mokhtar@Makhtar, Khairani Idah
title	RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study
title_short	RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study
title_full	RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study
title_fullStr	RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study
title_full_unstemmed	RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study
title_sort	runx2 single nucleotide polymorphism (rs6930053) in class ii malocclusions patients: a preliminary study
publishDate	2018
url	http://irep.iium.edu.my/66589/ http://irep.iium.edu.my/66589/ http://irep.iium.edu.my/66589/1/ACMBMB2018-Program-Book-3-pages-1%2C4%2C10%2C36%2C97.pdf http://irep.iium.edu.my/66589/2/KIM-ACMBMB%202018%20%282%29.pdf
first_indexed	2023-09-18T21:34:34Z
last_indexed	2023-09-18T21:34:34Z
_version_	1777412734796693504

RUNX2 single nucleotide polymorphism (rs6930053) in Class II malocclusions patients: a preliminary study

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