ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads
Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluron...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Innovare Academics Sciences Pvt. Ltd
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/1/66788_ATR-FTIR%20spectroscopic%20methods.pdf http://irep.iium.edu.my/66788/2/66788_ATR-FTIR%20spectroscopic%20methods_SCOPUS.pdf |
| Summary: | Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement.
Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer.
Results: ATR-FTIR method exhibited linearity in the range of 25-300 mgBSO/mlemulsion (mgBSO/mlE)with R2=0.998, RSD=8.4%, LOD=0.28 mgBSO/mlE and LOQ=0.87 mgBSO/mlE. BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mgBSO/mlE, R2=0.9931, RSD=1.34%, LOD=0.89 mgBSO/mlE, and LOQ=2.71 mgBSO/mlE. In addition, the method showed that total amount of BSO was released at 110 min.
Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology. |
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