Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity

Indomethacin is a potent, nonselective Non-steroidal Antiinflammatory Drug (NSAID) but its low water-solubility precludes its use as topical dosage form. As with other NSAIDs, the systemic delivery is associated with high risk of serious gastrointestinal adverse events including bleeding, ulcera...

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Main Authors:	Badri, Waisudin, Mohamed, Farahidah, Mohd Shafri, Mohd Affendi, El Asbahani, A., Miladi, Karim, Nazari, Qand Agha, Viennet, Celine, Robin, Sophie, Fessi, Hatem, Elaissari, Abdelhamid
Format:	Article
Language:	English
Published:	International Journal of Advanced Research (IJAR) 2018
Subjects:	RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/66791/ http://irep.iium.edu.my/66791/ http://irep.iium.edu.my/66791/ http://irep.iium.edu.my/66791/1/443_IJAR%20indomethacin%20microparticles.pdf

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recordtype	eprints
spelling	iium-667912018-10-19T08:22:01Z http://irep.iium.edu.my/66791/ Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity Badri, Waisudin Mohamed, Farahidah Mohd Shafri, Mohd Affendi El Asbahani, A. Miladi, Karim Nazari, Qand Agha Viennet, Celine Robin, Sophie Fessi, Hatem Elaissari, Abdelhamid RM Therapeutics. Pharmacology RS Pharmacy and materia medica Indomethacin is a potent, nonselective Non-steroidal Antiinflammatory Drug (NSAID) but its low water-solubility precludes its use as topical dosage form. As with other NSAIDs, the systemic delivery is associated with high risk of serious gastrointestinal adverse events including bleeding, ulceration and perforation of stomach and intestines. Here we demonstrate a safer way of administration i.e via topical demonstrating synergistic effects when co-delivered with Nigella sativa L. seeds essential oil (NSSEO) in the form of coencapsulated particles (~200 nm) of poly--caprolactone. The particles showed penetrability across stratum corneum to dermis layer in ex-vivo human skin. Further study in the xyline-induced ear edema in mice was performed, and co-encapsulated particles demonstrated highest antiinflammatory effect compared to indomethacin particles and indomethacin gels. Despite slower onset compared to indomethacin gels, the inflamed ear continued to show reduction in thickness over 8 hours of observation demonstrating synergistic and pro-longed effect contributed by NSSEO. In immunohistochemistry study of CD45+, the mice ears treated with co-encapsulated particles showed considerable reduction in lesions, epidermal-dermal separation and inflammatory cells (lymphocytes and neutrophils) infiltration as compared to other formulation. Based on microscopic evaluation, the anti-inflammatory inhibition effect of co-encapsulated particles is the highest (90%) followed by indomethacin particles (79%) and indomethacin gel (49%). The findings suggest not only skin permeability of indomethacin significantly improved but also the therapeutic effects, all provided by the presence of NSSEO in the particles. This study paves the way to more co-encapsulation of any other contemporary medicines in combination with this wholesome natural oil, NSSEO. International Journal of Advanced Research (IJAR) 2018-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66791/1/443_IJAR%20indomethacin%20microparticles.pdf Badri, Waisudin and Mohamed, Farahidah and Mohd Shafri, Mohd Affendi and El Asbahani, A. and Miladi, Karim and Nazari, Qand Agha and Viennet, Celine and Robin, Sophie and Fessi, Hatem and Elaissari, Abdelhamid (2018) Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity. International Journal of Advanced Research, 6 (4). pp. 801-816. ISSN 2320-5407 http://www.journalijar.com/uploads/443_IJAR-22995.pdf 10.21474/IJAR01/6916
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics. Pharmacology RS Pharmacy and materia medica Badri, Waisudin Mohamed, Farahidah Mohd Shafri, Mohd Affendi El Asbahani, A. Miladi, Karim Nazari, Qand Agha Viennet, Celine Robin, Sophie Fessi, Hatem Elaissari, Abdelhamid Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
description	Indomethacin is a potent, nonselective Non-steroidal Antiinflammatory Drug (NSAID) but its low water-solubility precludes its use as topical dosage form. As with other NSAIDs, the systemic delivery is associated with high risk of serious gastrointestinal adverse events including bleeding, ulceration and perforation of stomach and intestines. Here we demonstrate a safer way of administration i.e via topical demonstrating synergistic effects when co-delivered with Nigella sativa L. seeds essential oil (NSSEO) in the form of coencapsulated particles (~200 nm) of poly--caprolactone. The particles showed penetrability across stratum corneum to dermis layer in ex-vivo human skin. Further study in the xyline-induced ear edema in mice was performed, and co-encapsulated particles demonstrated highest antiinflammatory effect compared to indomethacin particles and indomethacin gels. Despite slower onset compared to indomethacin gels, the inflamed ear continued to show reduction in thickness over 8 hours of observation demonstrating synergistic and pro-longed effect contributed by NSSEO. In immunohistochemistry study of CD45+, the mice ears treated with co-encapsulated particles showed considerable reduction in lesions, epidermal-dermal separation and inflammatory cells (lymphocytes and neutrophils) infiltration as compared to other formulation. Based on microscopic evaluation, the anti-inflammatory inhibition effect of co-encapsulated particles is the highest (90%) followed by indomethacin particles (79%) and indomethacin gel (49%). The findings suggest not only skin permeability of indomethacin significantly improved but also the therapeutic effects, all provided by the presence of NSSEO in the particles. This study paves the way to more co-encapsulation of any other contemporary medicines in combination with this wholesome natural oil, NSSEO.
format	Article
author	Badri, Waisudin Mohamed, Farahidah Mohd Shafri, Mohd Affendi El Asbahani, A. Miladi, Karim Nazari, Qand Agha Viennet, Celine Robin, Sophie Fessi, Hatem Elaissari, Abdelhamid
author_facet	Badri, Waisudin Mohamed, Farahidah Mohd Shafri, Mohd Affendi El Asbahani, A. Miladi, Karim Nazari, Qand Agha Viennet, Celine Robin, Sophie Fessi, Hatem Elaissari, Abdelhamid
author_sort	Badri, Waisudin
title	Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
title_short	Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
title_full	Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
title_fullStr	Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
title_full_unstemmed	Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
title_sort	topical co-delivery of indomethacin and nigella sativa l. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity
publisher	International Journal of Advanced Research (IJAR)
publishDate	2018
url	http://irep.iium.edu.my/66791/ http://irep.iium.edu.my/66791/ http://irep.iium.edu.my/66791/ http://irep.iium.edu.my/66791/1/443_IJAR%20indomethacin%20microparticles.pdf
first_indexed	2023-09-18T21:34:51Z
last_indexed	2023-09-18T21:34:51Z
_version_	1777412752758800384

Topical co-delivery of indomethacin and nigella sativa L. essential oil in poly-cappa-caprolactone nanoparticles: in vitro study of anti-inflammatory activity

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