First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia

Aims: This study focus on the presence of cyanobacterial toxin in Malaysia and anatoxin-a-encoding gene was detected in this study and the status of cyanobacterial toxins in Malaysia can now be clarified. Methodology and results: As part of status determination of cyanobacterial toxins in Malaysia,...

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Main Authors:	Mohamad, Rohaslinda, Lalung, Japereng, Rafatullah, Mohd, Ismail, Norli, Abbas, Syed Zaghum, Zainuddin, Zarina
Format:	Article
Language:	English English English
Published:	Malaysian Society for Microbiology 2018
Subjects:	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/66823/ http://irep.iium.edu.my/66823/ http://irep.iium.edu.my/66823/1/66823_First%20report%20of%20anatoxin-a%20encoding.pdf http://irep.iium.edu.my/66823/2/66823_First%20report%20of%20anatoxin-a%20encoding_SCOPUS.pdf http://irep.iium.edu.my/66823/3/66823_First%20report%20of%20anatoxin-a%20encoding_WOS.pdf

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recordtype	eprints
spelling	iium-668232019-03-13T08:44:21Z http://irep.iium.edu.my/66823/ First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia Mohamad, Rohaslinda Lalung, Japereng Rafatullah, Mohd Ismail, Norli Abbas, Syed Zaghum Zainuddin, Zarina RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Aims: This study focus on the presence of cyanobacterial toxin in Malaysia and anatoxin-a-encoding gene was detected in this study and the status of cyanobacterial toxins in Malaysia can now be clarified. Methodology and results: As part of status determination of cyanobacterial toxins in Malaysia, cyanobacterial strains have been isolated from different environments and identified using cyanobacterial 16S rRNA gene sequence. PCR assay was carried out to detect the presence of cyanobacterial toxin-encoding genes in these isolated strains by amplifying genes encoded for microcystin, anatoxin-a, cylindrospermopsin and saxitoxin. Using molecular identification of 16S rRNA gene sequences, a total of forty-two cyanobacterial strains were identified, which belongs to eighteen different genera of Synechococcus, Cyanobium, Synechocystis, Chroococcidiopsis, Leptolyngbya, Nodosilinea, Limnothrix, Pseudanabaena, Cephalothrix, Aerosakkonema, Oscillatoria, Alkalinema, Pantanalinema, Planktolyngbya, Scytonema, Nostoc, Hapalosiphon and Symphyonemopsis. The toxicity of these strains was tested using PCR amplification of toxin-encoding genes using specific primers. Conclusion, significance and impact of study: Anatoxin-a (ATX) gene, which involved in the biosynthesis of anatoxin-A was detected in two isolated strains namely Limnothrix sp. B15 and Leptolyngbya sp. D1C10. This study focus on the the presence of cyanobacterial toxin in Malaysia can now be determined as potential threat because anatoxin-a-encoding gene was detected in this study and the status of cyanobacterial toxins in Malaysia can now be clarified. © 2018, Universiti Sains Malaysia. Malaysian Society for Microbiology 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66823/1/66823_First%20report%20of%20anatoxin-a%20encoding.pdf application/pdf en http://irep.iium.edu.my/66823/2/66823_First%20report%20of%20anatoxin-a%20encoding_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/66823/3/66823_First%20report%20of%20anatoxin-a%20encoding_WOS.pdf Mohamad, Rohaslinda and Lalung, Japereng and Rafatullah, Mohd and Ismail, Norli and Abbas, Syed Zaghum and Zainuddin, Zarina (2018) First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia. Malaysian Journal of Microbiology, 14 (2 (Special issue)). pp. 202-207. ISSN 1823-8262 E-ISSN 2231-7538 http://mjm.usm.my/uploads/issues/1402/MJM%202018-19-82.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology Mohamad, Rohaslinda Lalung, Japereng Rafatullah, Mohd Ismail, Norli Abbas, Syed Zaghum Zainuddin, Zarina First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia
description	Aims: This study focus on the presence of cyanobacterial toxin in Malaysia and anatoxin-a-encoding gene was detected in this study and the status of cyanobacterial toxins in Malaysia can now be clarified. Methodology and results: As part of status determination of cyanobacterial toxins in Malaysia, cyanobacterial strains have been isolated from different environments and identified using cyanobacterial 16S rRNA gene sequence. PCR assay was carried out to detect the presence of cyanobacterial toxin-encoding genes in these isolated strains by amplifying genes encoded for microcystin, anatoxin-a, cylindrospermopsin and saxitoxin. Using molecular identification of 16S rRNA gene sequences, a total of forty-two cyanobacterial strains were identified, which belongs to eighteen different genera of Synechococcus, Cyanobium, Synechocystis, Chroococcidiopsis, Leptolyngbya, Nodosilinea, Limnothrix, Pseudanabaena, Cephalothrix, Aerosakkonema, Oscillatoria, Alkalinema, Pantanalinema, Planktolyngbya, Scytonema, Nostoc, Hapalosiphon and Symphyonemopsis. The toxicity of these strains was tested using PCR amplification of toxin-encoding genes using specific primers. Conclusion, significance and impact of study: Anatoxin-a (ATX) gene, which involved in the biosynthesis of anatoxin-A was detected in two isolated strains namely Limnothrix sp. B15 and Leptolyngbya sp. D1C10. This study focus on the the presence of cyanobacterial toxin in Malaysia can now be determined as potential threat because anatoxin-a-encoding gene was detected in this study and the status of cyanobacterial toxins in Malaysia can now be clarified. © 2018, Universiti Sains Malaysia.
format	Article
author	Mohamad, Rohaslinda Lalung, Japereng Rafatullah, Mohd Ismail, Norli Abbas, Syed Zaghum Zainuddin, Zarina
author_facet	Mohamad, Rohaslinda Lalung, Japereng Rafatullah, Mohd Ismail, Norli Abbas, Syed Zaghum Zainuddin, Zarina
author_sort	Mohamad, Rohaslinda
title	First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia
title_short	First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia
title_full	First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia
title_fullStr	First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia
title_full_unstemmed	First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia
title_sort	first report of anatoxin-a encoding gene in isolated cyanobacterial strains from malaysia
publisher	Malaysian Society for Microbiology
publishDate	2018
url	http://irep.iium.edu.my/66823/ http://irep.iium.edu.my/66823/ http://irep.iium.edu.my/66823/1/66823_First%20report%20of%20anatoxin-a%20encoding.pdf http://irep.iium.edu.my/66823/2/66823_First%20report%20of%20anatoxin-a%20encoding_SCOPUS.pdf http://irep.iium.edu.my/66823/3/66823_First%20report%20of%20anatoxin-a%20encoding_WOS.pdf
first_indexed	2023-09-18T21:34:53Z
last_indexed	2023-09-18T21:34:53Z
_version_	1777412754806669312

First report of anatoxin-a encoding gene in isolated cyanobacterial strains from Malaysia

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