Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)

Biofilm complicates osteomyelitis as there is antibiotic resistance and toxicity involved. In order to overcome the challenges of current treatment, gentamicin, the current antibiotic of choice for osteomyelitis, is fused with Nigella sativa oil to form an emulsion. Its efficacy as anti-biofilm a...

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Main Authors:	Yaacob, Khairul Ikhwan, Mohd Shafri, Mohd Affendi, Mohd Yusof, Nazri, Mohamed, Farahidah
Format:	Article
Language:	English
Published:	Microscopy Society of Malaysia (MSM) 2015
Subjects:	QR Microbiology R Medicine (General) RM147 Administration of Drugs and Other Therapeutic Agents
Online Access:	http://irep.iium.edu.my/66948/ http://irep.iium.edu.my/66948/ http://irep.iium.edu.my/66948/1/MJMicroscopy-2015-12%20%282%29.pdf

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recordtype	eprints
spelling	iium-669482019-01-15T02:26:46Z http://irep.iium.edu.my/66948/ Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE) Yaacob, Khairul Ikhwan Mohd Shafri, Mohd Affendi Mohd Yusof, Nazri Mohamed, Farahidah QR Microbiology R Medicine (General) RM147 Administration of Drugs and Other Therapeutic Agents Biofilm complicates osteomyelitis as there is antibiotic resistance and toxicity involved. In order to overcome the challenges of current treatment, gentamicin, the current antibiotic of choice for osteomyelitis, is fused with Nigella sativa oil to form an emulsion. Its efficacy as anti-biofilm agent is assessed using confocal laser scanning microscope (CLSM) against different strains of biofilm-producing Staphylococcus aureus. These strains of S.aureus were first allowed to express biofilm before being exposed for 24 hours to the emulsion with (0.1% (w/v) gentamicin; 40.2% (v/v) N.sativa. Later, the emulsion was removed and the biofilm was stained with fluorescence staining. The slides were viewed under CLSM at 100 times resolution. 3D images of biofilm were reconstructed, using Image J software, to measure the thickness of biofilm and viability of bacteria cells. Results revealed that the emulsion significantly reduced biofilm thickness compared to gentamicin and N.sativa alone in all strains of S.aureus (Tukey’s test p < 0.05). The emulsion was also able to produce more than 80% and 15% surface percentage (%) of non-viable (dead) bacteria in the sensitive and resistant strain, respectively, at a significant level when compared to gentamicin and N.sativa (Tukey’s test p < 0.05). As a conclusion, this new fusion of gentamicin-N.sativa may be effective towards the biofilm of S.aureus, and can be developed further as a new promising anti-biofilm agent in osteomyelitis. Microscopy Society of Malaysia (MSM) 2015-12-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66948/1/MJMicroscopy-2015-12%20%282%29.pdf Yaacob, Khairul Ikhwan and Mohd Shafri, Mohd Affendi and Mohd Yusof, Nazri and Mohamed, Farahidah (2015) Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE). Malaysian Journal of Microscopy, 11. pp. 68-73. ISSN 1823-7010 E-ISSN 2600-7444 http://mjm.msm.org.my/index.php/mjm/index
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology R Medicine (General) RM147 Administration of Drugs and Other Therapeutic Agents
spellingShingle	QR Microbiology R Medicine (General) RM147 Administration of Drugs and Other Therapeutic Agents Yaacob, Khairul Ikhwan Mohd Shafri, Mohd Affendi Mohd Yusof, Nazri Mohamed, Farahidah Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
description	Biofilm complicates osteomyelitis as there is antibiotic resistance and toxicity involved. In order to overcome the challenges of current treatment, gentamicin, the current antibiotic of choice for osteomyelitis, is fused with Nigella sativa oil to form an emulsion. Its efficacy as anti-biofilm agent is assessed using confocal laser scanning microscope (CLSM) against different strains of biofilm-producing Staphylococcus aureus. These strains of S.aureus were first allowed to express biofilm before being exposed for 24 hours to the emulsion with (0.1% (w/v) gentamicin; 40.2% (v/v) N.sativa. Later, the emulsion was removed and the biofilm was stained with fluorescence staining. The slides were viewed under CLSM at 100 times resolution. 3D images of biofilm were reconstructed, using Image J software, to measure the thickness of biofilm and viability of bacteria cells. Results revealed that the emulsion significantly reduced biofilm thickness compared to gentamicin and N.sativa alone in all strains of S.aureus (Tukey’s test p < 0.05). The emulsion was also able to produce more than 80% and 15% surface percentage (%) of non-viable (dead) bacteria in the sensitive and resistant strain, respectively, at a significant level when compared to gentamicin and N.sativa (Tukey’s test p < 0.05). As a conclusion, this new fusion of gentamicin-N.sativa may be effective towards the biofilm of S.aureus, and can be developed further as a new promising anti-biofilm agent in osteomyelitis.
format	Article
author	Yaacob, Khairul Ikhwan Mohd Shafri, Mohd Affendi Mohd Yusof, Nazri Mohamed, Farahidah
author_facet	Yaacob, Khairul Ikhwan Mohd Shafri, Mohd Affendi Mohd Yusof, Nazri Mohamed, Farahidah
author_sort	Yaacob, Khairul Ikhwan
title	Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
title_short	Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
title_full	Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
title_fullStr	Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
title_full_unstemmed	Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)
title_sort	confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (gnfe)
publisher	Microscopy Society of Malaysia (MSM)
publishDate	2015
url	http://irep.iium.edu.my/66948/ http://irep.iium.edu.my/66948/ http://irep.iium.edu.my/66948/1/MJMicroscopy-2015-12%20%282%29.pdf
first_indexed	2023-09-18T21:35:03Z
last_indexed	2023-09-18T21:35:03Z
_version_	1777412764642312192

Confocal laser scanning microscope analysis on post-biofilm assessment of biofilmproducing osteomyelitic staphylococcus aureus treated with new gentamicin-nigella sativa fusion emulsion (GNFE)

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