Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance.
Alterations to the immune system caused by stress have been considered to markedly increase the risk for immune-related diseases such as cancer and autoimmune disorders. We investigated the potential anti-stress effects of the histamine 4 receptor (H4R) agonist, 4-methylhistamine (4-MeH), in a murin...
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| Format: | Article |
| Language: | English English |
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Elsevier
2015
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| Online Access: | http://irep.iium.edu.my/66949/ http://irep.iium.edu.my/66949/ http://irep.iium.edu.my/66949/ http://irep.iium.edu.my/66949/1/Stimulation%20of%20the%20histamine%204%20receptor%20with%204-methylhistamine%20modulates%20the%20effects%20of%20chronic%20stress%20on%20the%20Th1%20Th2%20cytokine%20balance.pdf http://irep.iium.edu.my/66949/7/66949_Scopus%20-%20Document%20details.pdf |
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iium-66949 |
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eprints |
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iium-669492019-01-04T09:01:36Z http://irep.iium.edu.my/66949/ Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. Ahmada, Sheikh Fayaz Zoheir, Khairy M.A. Ansari, Mushtaq Ahmad Korashya, Hesham M. Bakheet, Saleh A. Ashour, Abdelkader Elbadawy Abbas Attiaa,, Sabry M. RM300 Drugs and their action Alterations to the immune system caused by stress have been considered to markedly increase the risk for immune-related diseases such as cancer and autoimmune disorders. We investigated the potential anti-stress effects of the histamine 4 receptor (H4R) agonist, 4-methylhistamine (4-MeH), in a murine stress model. Mice were placed in 50ml conical centrifuge tubes for 12h followed by a 12h rest. The effects of treatment with 4-MeH (30mg/kg, i.p., twice daily) for 2 days were assessed. At 2 days after physical restraint, mice were sacrificed and tissues harvested. We evaluated the effects of 4-MeH treatment on CD4(+) T cell production, and intracellular IFN-γ and IL-4 expression in these cells. We also assessed IL-1β, IFN-γ, TNF-α, and IL-4 mRNA expression as well as IFN-γ, TNF-α, GITR, Ox40 and IL-4 protein expression in the spleen. The results showed that 4-MeH treatment of stressed mice results in a substantial increase in the CD4(+) T cells as well as in IFN-γ production by these cells. Compared to both untreated and stressed controls. In contrast, IL-4 expression decreased significantly following 4-MeH treatment of mice. Moreover, stimulation of the H4R resulted in up-regulated expression of IL-1β, IFN-γ and TNF-α mRNAs and decreased the expression of IL-4. Western blot analysis confirmed decreased protein expression of IFN-γ, TNF-α, GITR, Ox40 and increased IL-4 in the SC group and treatment of mice with 4-MeH reversed these effects. Our results confirm the significant impact of chronic stress on T cell function and production of Th1/Th2 mediators H4R. Elsevier 2015-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66949/1/Stimulation%20of%20the%20histamine%204%20receptor%20with%204-methylhistamine%20modulates%20the%20effects%20of%20chronic%20stress%20on%20the%20Th1%20Th2%20cytokine%20balance.pdf application/pdf en http://irep.iium.edu.my/66949/7/66949_Scopus%20-%20Document%20details.pdf Ahmada, Sheikh Fayaz and Zoheir, Khairy M.A. and Ansari, Mushtaq Ahmad and Korashya, Hesham M. and Bakheet, Saleh A. and Ashour, Abdelkader Elbadawy Abbas and Attiaa,, Sabry M. (2015) Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. Immunobiology, 220 (3). pp. 341-349. ISSN 0171-2985 E-ISSN 1878-3279 https://www.elsevier.com/journals/personal/immunobiology/0171-2985 10.1016/j.imbio.2014.10.014 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
RM300 Drugs and their action |
| spellingShingle |
RM300 Drugs and their action Ahmada, Sheikh Fayaz Zoheir, Khairy M.A. Ansari, Mushtaq Ahmad Korashya, Hesham M. Bakheet, Saleh A. Ashour, Abdelkader Elbadawy Abbas Attiaa,, Sabry M. Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. |
| description |
Alterations to the immune system caused by stress have been considered to markedly increase the risk for immune-related diseases such as cancer and autoimmune disorders. We investigated the potential anti-stress effects of the histamine 4 receptor (H4R) agonist, 4-methylhistamine (4-MeH), in a murine stress model. Mice were placed in 50ml conical centrifuge tubes for 12h followed by a 12h rest. The effects of treatment with 4-MeH (30mg/kg, i.p., twice daily) for 2 days were assessed. At 2 days after physical restraint, mice were sacrificed and tissues harvested. We evaluated the effects of 4-MeH treatment on CD4(+) T cell production, and intracellular IFN-γ and IL-4 expression in these cells. We also assessed IL-1β, IFN-γ, TNF-α, and IL-4 mRNA expression as well as IFN-γ, TNF-α, GITR, Ox40 and IL-4 protein expression in the spleen. The results showed that 4-MeH treatment of stressed mice results in a substantial increase in the CD4(+) T cells as well as in IFN-γ production by these cells. Compared to both untreated and stressed controls. In contrast, IL-4 expression decreased significantly following 4-MeH treatment of mice. Moreover, stimulation of the H4R resulted in up-regulated expression of IL-1β, IFN-γ and TNF-α mRNAs and decreased the expression of IL-4. Western blot analysis confirmed decreased protein expression of IFN-γ, TNF-α, GITR, Ox40 and increased IL-4 in the SC group and treatment of mice with 4-MeH reversed these effects. Our results confirm the significant impact of chronic stress on T cell function and production of Th1/Th2 mediators H4R. |
| format |
Article |
| author |
Ahmada, Sheikh Fayaz Zoheir, Khairy M.A. Ansari, Mushtaq Ahmad Korashya, Hesham M. Bakheet, Saleh A. Ashour, Abdelkader Elbadawy Abbas Attiaa,, Sabry M. |
| author_facet |
Ahmada, Sheikh Fayaz Zoheir, Khairy M.A. Ansari, Mushtaq Ahmad Korashya, Hesham M. Bakheet, Saleh A. Ashour, Abdelkader Elbadawy Abbas Attiaa,, Sabry M. |
| author_sort |
Ahmada, Sheikh Fayaz |
| title |
Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. |
| title_short |
Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. |
| title_full |
Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. |
| title_fullStr |
Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. |
| title_full_unstemmed |
Stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the Th1/Th2 cytokine balance. |
| title_sort |
stimulation of the histamine 4 receptor with 4-methylhistamine modulates the effects of chronic stress on the th1/th2 cytokine balance. |
| publisher |
Elsevier |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/66949/ http://irep.iium.edu.my/66949/ http://irep.iium.edu.my/66949/ http://irep.iium.edu.my/66949/1/Stimulation%20of%20the%20histamine%204%20receptor%20with%204-methylhistamine%20modulates%20the%20effects%20of%20chronic%20stress%20on%20the%20Th1%20Th2%20cytokine%20balance.pdf http://irep.iium.edu.my/66949/7/66949_Scopus%20-%20Document%20details.pdf |
| first_indexed |
2023-09-18T21:35:03Z |
| last_indexed |
2023-09-18T21:35:03Z |
| _version_ |
1777412764791209984 |