Shedding light on lipase stability in natural deep eutectic solvents

This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as gly...

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Main Authors:	Elgharbawy, Amal Ahmad, Hayyan, Adeeb, Hayyan, Maan, Rashid, Shahidah Nusailah, Nor, M.R.M., Zulkifli, M.Y., Alias, Yatimah, Mirghani, Mohamed Elwathig Saeed
Format:	Article
Language:	English English
Published:	Croation Society of Chemical Engineers 2018
Subjects:	QD Chemistry T Technology (General)
Online Access:	http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf

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spelling	iium-670152019-03-14T03:41:24Z http://irep.iium.edu.my/67015/ Shedding light on lipase stability in natural deep eutectic solvents Elgharbawy, Amal Ahmad Hayyan, Adeeb Hayyan, Maan Rashid, Shahidah Nusailah Nor, M.R.M. Zulkifli, M.Y. Alias, Yatimah Mirghani, Mohamed Elwathig Saeed QD Chemistry T Technology (General) This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents. Croation Society of Chemical Engineers 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf application/pdf en http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf Elgharbawy, Amal Ahmad and Hayyan, Adeeb and Hayyan, Maan and Rashid, Shahidah Nusailah and Nor, M.R.M. and Zulkifli, M.Y. and Alias, Yatimah and Mirghani, Mohamed Elwathig Saeed (2018) Shedding light on lipase stability in natural deep eutectic solvents. Chemical and Biochemical Engineering Quarterly, 32 (3). pp. 359-370. ISSN 0352-9568 E-ISSN 1846-5153 http://silverstripe.fkit.hr/cabeq/past-issues/article/1515 10.15255/CABEQ.2018.1335
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry T Technology (General)
spellingShingle	QD Chemistry T Technology (General) Elgharbawy, Amal Ahmad Hayyan, Adeeb Hayyan, Maan Rashid, Shahidah Nusailah Nor, M.R.M. Zulkifli, M.Y. Alias, Yatimah Mirghani, Mohamed Elwathig Saeed Shedding light on lipase stability in natural deep eutectic solvents
description	This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. Ammonium salts such as choline chloride (ChCl) were paired with different hydrogen bond donors such as glycerol and malonic acid and sugars (glucose, fructose and sucrose). The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. The results showed that NADES3 prepared from ChCl/sucrose was the most promising solvent as it enhanced the activities of both CALB and lipase from porcine pancreas to 355 % and 345 %. The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. The trend achieved by NADES may be a promising approach for applications and further perspectives as genuinely green industrial solvents.
format	Article
author	Elgharbawy, Amal Ahmad Hayyan, Adeeb Hayyan, Maan Rashid, Shahidah Nusailah Nor, M.R.M. Zulkifli, M.Y. Alias, Yatimah Mirghani, Mohamed Elwathig Saeed
author_facet	Elgharbawy, Amal Ahmad Hayyan, Adeeb Hayyan, Maan Rashid, Shahidah Nusailah Nor, M.R.M. Zulkifli, M.Y. Alias, Yatimah Mirghani, Mohamed Elwathig Saeed
author_sort	Elgharbawy, Amal Ahmad
title	Shedding light on lipase stability in natural deep eutectic solvents
title_short	Shedding light on lipase stability in natural deep eutectic solvents
title_full	Shedding light on lipase stability in natural deep eutectic solvents
title_fullStr	Shedding light on lipase stability in natural deep eutectic solvents
title_full_unstemmed	Shedding light on lipase stability in natural deep eutectic solvents
title_sort	shedding light on lipase stability in natural deep eutectic solvents
publisher	Croation Society of Chemical Engineers
publishDate	2018
url	http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/ http://irep.iium.edu.my/67015/1/elgharbawy%20et%20al%202018.pdf http://irep.iium.edu.my/67015/7/67015_Shedding%20light%20on%20lipase%20stability%20in%20natural%20_scopus.pdf
first_indexed	2023-09-18T21:35:09Z
last_indexed	2023-09-18T21:35:09Z
_version_	1777412770808987648

Shedding light on lipase stability in natural deep eutectic solvents

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