Isolation and identification of bacteria-producing cellulose from tropical fruit for Halal capsule application
Bacterial cellulose (BC) is pure cellulose synthesized by various species of bacteria. Raising demands on bacterial cellulose is due to its simpler and pure structure. It has plenty of applications in various industries such as food, medical and cosmetics, thus, allowing it to be the choice in ha...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/67046/ http://irep.iium.edu.my/67046/ http://irep.iium.edu.my/67046/1/67046_Isolation%20and%20Identification%20of%20Bacteria-producing.pdf |
| Summary: | Bacterial cellulose (BC) is pure cellulose synthesized by various species of bacteria.
Raising demands on bacterial cellulose is due to its simpler and pure structure. It has plenty of
applications in various industries such as food, medical and cosmetics, thus, allowing it to be
the choice in halal industries application as a substitute for non-halal gelatine. However,
challenges arise during the BC production such as high production cost and low volumetric
yield. In this study, BC is studied to overcome the barriers in BC production. This present study
carried out the isolation and identification of cellulose producing bacteria from selected tropical
fruits. The bacteria were isolated from eight different tropical fruit sources using HestrinSchramm
media in static culture condition. Morphological and molecular identification by
microscopic observation, gram staining, and 16S rRNA analysis were conducted to identify the
characteristics and strain of the new isolate. Next, the selected colonies were challenged to
grow in agitating condition using modified HS media. The effects of carbon concentration and
agitation speed on the production of cellulose were investigated using on central composite
design (CCD). Characterization of BC was carried out by scanning electron microscopy (SEM)
and Fourier Transform Infrared spectroscopy (FTIR) analysis. The result indicated that three
new cellulose-producing bacteria were successfully isolated and similar to Enterobacter sp.
SJZ-5, Bacterium sp NLAE-zl-H356, and Bulkhoderia sp. RD_DACAR_02 through
morphological and molecular analysis. The most potent strain; which is similar to Enterobacter
sp. SJZ-5 was named as Enterobacter sp.M003 has been chosen for BC optimization study for
high BC production using modified HS media. Optimization of bacterial cellulose production
using response surface methodology (RSM) with 13 runs indicated that the optimal production
parameters are 17.57 g/L for carbon concentration in 277 rpm for agitation speed. It is expected
that the newly isolated bacteria will be able to provide an alternative to gelatine for halal
capsule production, thus minimizing non-halal gelatine usage. |
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