Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L.
Purpose – The purpose of this paper is to validate the antimicrobial activity (both antibacterial and antifungal) of in vivo and in vitro ethanolic anthocyanin extracts of Clitoria ternatea L. (vivid blue flower butterfly-pea) and Dioscorea alata L. (purple yam) against selected bacteria (Bacillus s...
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Emerald Group Publishing Ltd.
2018
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iium-67643 |
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| recordtype |
eprints |
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Digital Repository |
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Local University |
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International Islamic University Malaysia |
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IIUM Repository |
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Online Access |
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English English English |
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RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc |
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RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Abdullah, Sakinah Anuar, Nordiyanah Elias, Hashimah Rawi, Norlina Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. |
| description |
Purpose – The purpose of this paper is to validate the antimicrobial activity (both antibacterial and antifungal) of in vivo and in vitro ethanolic anthocyanin extracts of Clitoria ternatea L. (vivid blue flower butterfly-pea) and Dioscorea alata L. (purple yam) against selected bacteria (Bacillus subtilis, Staphylococcus aureus and Escherichia coli) and fungi (Fusarium sp., Aspergillus niger and Trichoderma sp.).
Design/methodology/approach – The freeze-dried samples (0.2 g) from in vivo vivid blue flowers of C. ternatea L. were extracted using 10 mL ethanol (produced ethanolic red extraction) and 10 mL distilled water (produced aqueous blue extraction) separately. Two-month-old in vitro callus
samples (0.2 g) were only extracted using 10 mL ethanol. The anthocyanin extractions were separated with the addition (several times) of ethyl acetate and distilled water (1:2:3) to remove stilbenoids, chlorophyll, less polar flavonoids and other non-polar compounds. Furthermore, the antimicrobial properties were determined using agar diffusion technique. Three bacteria (B.subtilis, S. aureus and E. coli) and fungi (F. sp., A. niger and T. sp.) were streaked on bacteria agar and dextrose agar, respectively, using “hockey stick”. Then, the sterile paper discs (6 mm diameter) were pipetted with 20 mL of 1,010 CFU/mL chloramphenicol (as a control for antibacterial) and carbendazim (as a control for antifungal) in vivo and in vitro extracts. The plates were incubated at room temperature for 48 h, and the inhibition zones were measured.
Findings – Based on the results, both in vivo and in vitro ethanolic extracts from vivid blue flowers of C. ternatea L. showed the best antibacterial activity against the same bacteria (B. subtilis), 11 and 10 mm inhibition zones, respectively. However, different antifungal activity was detected in in vitro ethanolic callus extract (12 mm), which was against T. sp., contrary to in vivo ethanolic extract (10 mm), which was against F. sp.; antibacterial
activity of D. alata L. was seen against the same bacteria (E. coli) with the highest inhibition zone for in vivo extract (8.8 mm), followed by in vitro extract (7.8 mm).
Research limitations/implications – Anthocyanins are responsible for the water-soluble and vacuolar, pink, red, purple and blue pigments present in coloured plant pigments. These pigments (pink, red, purple and blue) are of important agronomic value in many crops and ornamental plants. However, anthocyanins are not stable and are easy to degrade and fade whenever exposed to light.
Social implications – Plant extracts containing bioactive agents with antimicrobial properties have been found to be useful in treating bacterial and fungal infections, as well as showed multiple antibiotic resistance.
Originality/value – Both in vivo and in vitro extracts from vivid blue flower petals (C. ternatea L.) and purple yam (D. alata L.) have important applications as natural antimicrobial (antibacterial and antifungal) agents in the coating industry, instead of natural pharmaceutical products. |
| format |
Article |
| author |
Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Abdullah, Sakinah Anuar, Nordiyanah Elias, Hashimah Rawi, Norlina |
| author_facet |
Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Abdullah, Sakinah Anuar, Nordiyanah Elias, Hashimah Rawi, Norlina |
| author_sort |
Mahmad, Noraini |
| title |
Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. |
| title_short |
Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. |
| title_full |
Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. |
| title_fullStr |
Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. |
| title_full_unstemmed |
Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. |
| title_sort |
anthocyanin as potential source for antimicrobial activity in clitoria ternatea l. and dioscorea alata l. |
| publisher |
Emerald Group Publishing Ltd. |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/67643/ http://irep.iium.edu.my/67643/ http://irep.iium.edu.my/67643/ http://irep.iium.edu.my/67643/1/67643_Anthocyanin%20as%20potential%20source%20for%20antimicrobial.pdf http://irep.iium.edu.my/67643/2/67643_Anthocyanin%20as%20potential%20source%20for%20antimicrobial_SCOPUS.pdf http://irep.iium.edu.my/67643/3/67643_Anthocyanin%20as%20potential%20source%20for%20antimicrobial_WOS.pdf |
| first_indexed |
2023-09-18T21:36:01Z |
| last_indexed |
2023-09-18T21:36:01Z |
| _version_ |
1777412825372688384 |
| spelling |
iium-676432018-12-31T12:14:15Z http://irep.iium.edu.my/67643/ Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Abdullah, Sakinah Anuar, Nordiyanah Elias, Hashimah Rawi, Norlina RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Purpose – The purpose of this paper is to validate the antimicrobial activity (both antibacterial and antifungal) of in vivo and in vitro ethanolic anthocyanin extracts of Clitoria ternatea L. (vivid blue flower butterfly-pea) and Dioscorea alata L. (purple yam) against selected bacteria (Bacillus subtilis, Staphylococcus aureus and Escherichia coli) and fungi (Fusarium sp., Aspergillus niger and Trichoderma sp.). Design/methodology/approach – The freeze-dried samples (0.2 g) from in vivo vivid blue flowers of C. ternatea L. were extracted using 10 mL ethanol (produced ethanolic red extraction) and 10 mL distilled water (produced aqueous blue extraction) separately. Two-month-old in vitro callus samples (0.2 g) were only extracted using 10 mL ethanol. The anthocyanin extractions were separated with the addition (several times) of ethyl acetate and distilled water (1:2:3) to remove stilbenoids, chlorophyll, less polar flavonoids and other non-polar compounds. Furthermore, the antimicrobial properties were determined using agar diffusion technique. Three bacteria (B.subtilis, S. aureus and E. coli) and fungi (F. sp., A. niger and T. sp.) were streaked on bacteria agar and dextrose agar, respectively, using “hockey stick”. Then, the sterile paper discs (6 mm diameter) were pipetted with 20 mL of 1,010 CFU/mL chloramphenicol (as a control for antibacterial) and carbendazim (as a control for antifungal) in vivo and in vitro extracts. The plates were incubated at room temperature for 48 h, and the inhibition zones were measured. Findings – Based on the results, both in vivo and in vitro ethanolic extracts from vivid blue flowers of C. ternatea L. showed the best antibacterial activity against the same bacteria (B. subtilis), 11 and 10 mm inhibition zones, respectively. However, different antifungal activity was detected in in vitro ethanolic callus extract (12 mm), which was against T. sp., contrary to in vivo ethanolic extract (10 mm), which was against F. sp.; antibacterial activity of D. alata L. was seen against the same bacteria (E. coli) with the highest inhibition zone for in vivo extract (8.8 mm), followed by in vitro extract (7.8 mm). Research limitations/implications – Anthocyanins are responsible for the water-soluble and vacuolar, pink, red, purple and blue pigments present in coloured plant pigments. These pigments (pink, red, purple and blue) are of important agronomic value in many crops and ornamental plants. However, anthocyanins are not stable and are easy to degrade and fade whenever exposed to light. Social implications – Plant extracts containing bioactive agents with antimicrobial properties have been found to be useful in treating bacterial and fungal infections, as well as showed multiple antibiotic resistance. Originality/value – Both in vivo and in vitro extracts from vivid blue flower petals (C. ternatea L.) and purple yam (D. alata L.) have important applications as natural antimicrobial (antibacterial and antifungal) agents in the coating industry, instead of natural pharmaceutical products. Emerald Group Publishing Ltd. 2018-11-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67643/1/67643_Anthocyanin%20as%20potential%20source%20for%20antimicrobial.pdf application/pdf en http://irep.iium.edu.my/67643/2/67643_Anthocyanin%20as%20potential%20source%20for%20antimicrobial_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/67643/3/67643_Anthocyanin%20as%20potential%20source%20for%20antimicrobial_WOS.pdf Mahmad, Noraini and Mat Taha, Rosna and Othman, Rashidi and Abdullah, Sakinah and Anuar, Nordiyanah and Elias, Hashimah and Rawi, Norlina (2018) Anthocyanin as potential source for antimicrobial activity in Clitoria ternatea L. and Dioscorea alata L. Pigment and Resin Technology, 47 (6). pp. 490-495. ISSN 0369-9420 https://www.emeraldinsight.com/doi/pdfplus/10.1108/PRT-11-2016-0109 10.1108/PRT-11-2016-0109 |