Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12S mitochondrial rRNA gene
Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
UPM Press
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/67774/ http://irep.iium.edu.my/67774/ http://irep.iium.edu.my/67774/1/2018%20JTAS%20Kamarudin%20et%20al.%2012S%20Processed%20Sea%20Cucumber.pdf http://irep.iium.edu.my/67774/7/67774_Species%20identification%20and%20molecular_scopus.pdf |
| Summary: | Extensive processing of sea cucumber causes body deformation of the marine organism,
hence causing difficulties in species identification of processed sea cucumbers. Due to
the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian
markets and worldwide, a study was conducted to determine the species identities of
processed sea cucumbers from selected Malaysian markets using non-protein-coding 12S
mitochondrial rRNA gene. Phylogenetic analyses based on the distance-based Neighbour
Joining method, and the character-based methods i.e. the Maximum Parsimony method,
Maximum Likelihood method, and the Bayesian Analysis method of 81 ingroup sequences
representing 63 processed sea cucumber specimens, 13 fresh and processed reference samples
for species identification, and five fresh additional specimens from Teluk Nipah Beach,
Pangkor Archipelago and Manukan Island, Sabah suggested the presence of three main
clusters i.e. a gamat family cluster consisting of family Stichopodidae (Stichopus horrens,
Stichopus vastus, and Thelenota anax) and two clusters of timun laut family comprising
family Holothuriidae (Holothuria
(Mertensiothuria) leucospilota, Holothuria
(Metriatyla)scabra, Holothuria (Metriatyla)
lessoni, Holothuria (Halodeima) atra, and
Holothuria (Halodeima) edulis) and family
Caudinidae (Acaudina molpadioides). The
outcomes of this study also highlighted the
availability of 40 new 12S mitochondrial
rRNA gene sequences deposited in the GenBank that can be utilised by the
enforcement agencies to monitor and
overcome the issues of species substitution
and product mislabelling of processed sea
cucumber products in Malaysian markets. |
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