Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells

Microbial infection has been shown to involve in oral carcinogenesis; however, the underlying mechanisms remain poorly understood. The present study aimed to characterize the growth of oral microorganisms as both monospecies and polymicrobial biofilms and determine the effects of their products on...

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Main Authors:	Arzmi, Mohd Hafiz, Nicola, Cirillo, Lenzo, Jason, Catmull, Deanne, O'Brien-Simpson, Neil, Reynolds, Eric, Dashper, Stuart, McCullough, Michael
Format:	Article
Language:	English English English
Published:	Oxford Univ Press 2019
Subjects:	QR Microbiology QR180 Immunology RK Dentistry
Online Access:	http://irep.iium.edu.my/67820/ http://irep.iium.edu.my/67820/ http://irep.iium.edu.my/67820/ http://irep.iium.edu.my/67820/25/67820_Monospecies%20and%20polymicrobial%20biofilms_article.pdf http://irep.iium.edu.my/67820/8/67820_Monospecies%20and%20polymicrobial%20biofilms_wos.pdf http://irep.iium.edu.my/67820/9/67820_Monospecies%20and%20polymicrobial%20biofilms_scopus.pdf

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recordtype	eprints
spelling	iium-678202019-07-31T03:44:27Z http://irep.iium.edu.my/67820/ Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells Arzmi, Mohd Hafiz Nicola, Cirillo Lenzo, Jason Catmull, Deanne O'Brien-Simpson, Neil Reynolds, Eric Dashper, Stuart McCullough, Michael QR Microbiology QR180 Immunology RK Dentistry Microbial infection has been shown to involve in oral carcinogenesis; however, the underlying mechanisms remain poorly understood. The present study aimed to characterize the growth of oral microorganisms as both monospecies and polymicrobial biofilms and determine the effects of their products on oral keratinocytes. Candida albicans (ALC3), Actinomyces naeslundii (AN) and Streptococcus mutans (SM) biofilms or a combination of these (TRI) were grown in flow-cell system for 24 h. The biofilms were subjected to fluorescent in situ hybridization using species-specific probes and analysed using confocal laser scanning microscopy. The effluent derived from each biofilm was collected and incubated with malignant (H357) and normal (OKF6) oral keratinocytes to assess extracellular matrix adhesion, epithelial–mesenchymal transition (EMT) and cytokines expression. Incubation of OKF6 with ALC3 and TRI effluent significantly decreased adhesion of the oral keratinocyte to collagen I, whereas incubation of H357 with similar effluent increased adhesion of the oral keratinocyte to laminin I, significantly when compared with incubation with artificial saliva containing serum-free medium (NE; P < 0.05). In OKF6, changes in E-cadherin and vimentin expression were not consistent with EMT although there was evidence of a mesenchymal to epithelial transition in malignant oral keratinocytes incubated with AN and SM effluent. A significant increase of pro-inflammatory cytokines expression, particularly interleukin (IL)-6 and IL-8, was observed when H357 was incubated with all biofilm effluents after 2- and 24-h incubation when compared with NE (P < 0.05). In conclusion, C.albicans, A.naeslundii and S.mutans form polymicrobial biofilms which differentially modulate malignant phenotype of oral keratinocytes. Oxford Univ Press 2019-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67820/25/67820_Monospecies%20and%20polymicrobial%20biofilms_article.pdf application/pdf en http://irep.iium.edu.my/67820/8/67820_Monospecies%20and%20polymicrobial%20biofilms_wos.pdf application/pdf en http://irep.iium.edu.my/67820/9/67820_Monospecies%20and%20polymicrobial%20biofilms_scopus.pdf Arzmi, Mohd Hafiz and Nicola, Cirillo and Lenzo, Jason and Catmull, Deanne and O'Brien-Simpson, Neil and Reynolds, Eric and Dashper, Stuart and McCullough, Michael (2019) Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells. Carcinogenesis, 40 (1). pp. 184-193. ISSN 0143-3334 E-ISSN 1460-2180 https://academic.oup.com/carcin/article-abstract/40/1/184/5182289?redirectedFrom=fulltext 10.1093/carcin/bgy137
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology QR180 Immunology RK Dentistry
spellingShingle	QR Microbiology QR180 Immunology RK Dentistry Arzmi, Mohd Hafiz Nicola, Cirillo Lenzo, Jason Catmull, Deanne O'Brien-Simpson, Neil Reynolds, Eric Dashper, Stuart McCullough, Michael Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
description	Microbial infection has been shown to involve in oral carcinogenesis; however, the underlying mechanisms remain poorly understood. The present study aimed to characterize the growth of oral microorganisms as both monospecies and polymicrobial biofilms and determine the effects of their products on oral keratinocytes. Candida albicans (ALC3), Actinomyces naeslundii (AN) and Streptococcus mutans (SM) biofilms or a combination of these (TRI) were grown in flow-cell system for 24 h. The biofilms were subjected to fluorescent in situ hybridization using species-specific probes and analysed using confocal laser scanning microscopy. The effluent derived from each biofilm was collected and incubated with malignant (H357) and normal (OKF6) oral keratinocytes to assess extracellular matrix adhesion, epithelial–mesenchymal transition (EMT) and cytokines expression. Incubation of OKF6 with ALC3 and TRI effluent significantly decreased adhesion of the oral keratinocyte to collagen I, whereas incubation of H357 with similar effluent increased adhesion of the oral keratinocyte to laminin I, significantly when compared with incubation with artificial saliva containing serum-free medium (NE; P < 0.05). In OKF6, changes in E-cadherin and vimentin expression were not consistent with EMT although there was evidence of a mesenchymal to epithelial transition in malignant oral keratinocytes incubated with AN and SM effluent. A significant increase of pro-inflammatory cytokines expression, particularly interleukin (IL)-6 and IL-8, was observed when H357 was incubated with all biofilm effluents after 2- and 24-h incubation when compared with NE (P < 0.05). In conclusion, C.albicans, A.naeslundii and S.mutans form polymicrobial biofilms which differentially modulate malignant phenotype of oral keratinocytes.
format	Article
author	Arzmi, Mohd Hafiz Nicola, Cirillo Lenzo, Jason Catmull, Deanne O'Brien-Simpson, Neil Reynolds, Eric Dashper, Stuart McCullough, Michael
author_facet	Arzmi, Mohd Hafiz Nicola, Cirillo Lenzo, Jason Catmull, Deanne O'Brien-Simpson, Neil Reynolds, Eric Dashper, Stuart McCullough, Michael
author_sort	Arzmi, Mohd Hafiz
title	Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
title_short	Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
title_full	Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
title_fullStr	Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
title_full_unstemmed	Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
title_sort	monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells
publisher	Oxford Univ Press
publishDate	2019
url	http://irep.iium.edu.my/67820/ http://irep.iium.edu.my/67820/ http://irep.iium.edu.my/67820/ http://irep.iium.edu.my/67820/25/67820_Monospecies%20and%20polymicrobial%20biofilms_article.pdf http://irep.iium.edu.my/67820/8/67820_Monospecies%20and%20polymicrobial%20biofilms_wos.pdf http://irep.iium.edu.my/67820/9/67820_Monospecies%20and%20polymicrobial%20biofilms_scopus.pdf
first_indexed	2023-09-18T21:36:18Z
last_indexed	2023-09-18T21:36:18Z
_version_	1777412843233083392

Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells

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