Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate

Microﬁbrillated cellulose (MFC) is a promising biomaterial in bionanocomposites production because of its renewable and eco-friendly properties. Conventional methods to produce MFC such as acid hydrolysis and mechanical treatment posed significant drawbacks; large amount of acid is required and incr...

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Main Authors:	Jimat, Dzun Noraini, Salleh, Norhidayah, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	IIUM Press, International Islamic University Malaysia 2018
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/67996/ http://irep.iium.edu.my/67996/ http://irep.iium.edu.my/67996/1/67996_Screening%20of%20parameters%20for%20solid-state.pdf

id	iium-67996
recordtype	eprints
spelling	iium-679962019-07-11T02:20:37Z http://irep.iium.edu.my/67996/ Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate Jimat, Dzun Noraini Salleh, Norhidayah Jami, Mohammed Saedi TP Chemical technology TP248.13 Biotechnology Microﬁbrillated cellulose (MFC) is a promising biomaterial in bionanocomposites production because of its renewable and eco-friendly properties. Conventional methods to produce MFC such as acid hydrolysis and mechanical treatment posed significant drawbacks; large amount of acid is required and increased defibrillation of alpha cellulose, respectively. Therefore, an appropriate method is very important in obtaining desired features of MFC such as high mechanical strength, high surface and low density properties. The utilization of agro-industry wastes such as sugarcane bagasse for MFC production could help to reduce the problem of lignocellulosic wastes. Thus, this study aimed to treat the sugarcane bagasse using fungal cellulases hydrolysis as a pretreatment method thus assisting the fibrillation of fiber cellulose via ultrasonication. The effects of solid-state fermentation of Aspergillus niger on cellulases activities and morphological changes of sugarcane bagasse (SCB) were investigated. Variables such as sugarcane bagasse particle size (400 and 600 µm), inoculum size (2% and 5%), medium pH (5 and 7), and fermentation time (5 and 15 days) were screened using 2-level factorial design (Design expert software, Stat-Ease Inc., Version 8.0). Analysis of variance (ANOVA) showed that the most significant parameter that affected the structural changes of sugarcane bagasse was its particle size. The increased glucose production indicated the increased of defibrillation of sugarcane bagasse. The use of fungal pretreatment through solid state fermentation of Aspergillus niger prior to ultrasonication was found to assist the later process in fibrillating sugarcane fibres. The results obtained from enzyme assays, optical microscope, SEM and FTIR showed the supporting evidence about the successful fibrillation cellulose of SCB. IIUM Press, International Islamic University Malaysia 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67996/1/67996_Screening%20of%20parameters%20for%20solid-state.pdf Jimat, Dzun Noraini and Salleh, Norhidayah and Jami, Mohammed Saedi (2018) Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate. Biological and Natural Resources Engineering Journal, 1 (1). pp. 26-35. E-ISSN 2637-0719 http://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/21/4
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Jimat, Dzun Noraini Salleh, Norhidayah Jami, Mohammed Saedi Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
description	Microﬁbrillated cellulose (MFC) is a promising biomaterial in bionanocomposites production because of its renewable and eco-friendly properties. Conventional methods to produce MFC such as acid hydrolysis and mechanical treatment posed significant drawbacks; large amount of acid is required and increased defibrillation of alpha cellulose, respectively. Therefore, an appropriate method is very important in obtaining desired features of MFC such as high mechanical strength, high surface and low density properties. The utilization of agro-industry wastes such as sugarcane bagasse for MFC production could help to reduce the problem of lignocellulosic wastes. Thus, this study aimed to treat the sugarcane bagasse using fungal cellulases hydrolysis as a pretreatment method thus assisting the fibrillation of fiber cellulose via ultrasonication. The effects of solid-state fermentation of Aspergillus niger on cellulases activities and morphological changes of sugarcane bagasse (SCB) were investigated. Variables such as sugarcane bagasse particle size (400 and 600 µm), inoculum size (2% and 5%), medium pH (5 and 7), and fermentation time (5 and 15 days) were screened using 2-level factorial design (Design expert software, Stat-Ease Inc., Version 8.0). Analysis of variance (ANOVA) showed that the most significant parameter that affected the structural changes of sugarcane bagasse was its particle size. The increased glucose production indicated the increased of defibrillation of sugarcane bagasse. The use of fungal pretreatment through solid state fermentation of Aspergillus niger prior to ultrasonication was found to assist the later process in fibrillating sugarcane fibres. The results obtained from enzyme assays, optical microscope, SEM and FTIR showed the supporting evidence about the successful fibrillation cellulose of SCB.
format	Article
author	Jimat, Dzun Noraini Salleh, Norhidayah Jami, Mohammed Saedi
author_facet	Jimat, Dzun Noraini Salleh, Norhidayah Jami, Mohammed Saedi
author_sort	Jimat, Dzun Noraini
title	Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_short	Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_full	Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_fullStr	Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_full_unstemmed	Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate
title_sort	screening of parameters for solid-state fermentation of aspergillus niger producing cellulose using sugarcane bagasse as substrate
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/67996/ http://irep.iium.edu.my/67996/ http://irep.iium.edu.my/67996/1/67996_Screening%20of%20parameters%20for%20solid-state.pdf
first_indexed	2023-09-18T21:36:32Z
last_indexed	2023-09-18T21:36:32Z
_version_	1777412858240303104

Screening of parameters for solid-state fermentation of Aspergillus niger producing cellulose using sugarcane bagasse as substrate

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