Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form
Introduction: Amlodipine (AMLO) and Perindopril (PER) have been combined in one tablet dosage form which has been proven to be an effective and well tolerated antihypertensive. Many analytical methods were applied for the analysis of AMLO and PER in this combination but most of the methods a...
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| Online Access: | http://irep.iium.edu.my/68161/ http://irep.iium.edu.my/68161/1/IPRC-2018-2.pdf |
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iium-681612018-12-18T07:20:46Z http://irep.iium.edu.my/68161/ Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form Alaama, Mohamed Helaluddin, Abul Bashar Mohammed Awang, Mohamed Abbas, Syed Atif QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Introduction: Amlodipine (AMLO) and Perindopril (PER) have been combined in one tablet dosage form which has been proven to be an effective and well tolerated antihypertensive. Many analytical methods were applied for the analysis of AMLO and PER in this combination but most of the methods are time and money consuming. A new sensitive, rapid, and specific LC- MS/MS analytical methods for the analysis of AMLO and PER was developed and validated. Chromatographic conditions for the best results were optimized using design of experiment method. 4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018 Challenging The Inquisitive Minds: Frontier Of The Future 24 – 26 August 2018 | Cyberjaya, Malaysia Method: The instrument used was Waters Aquity UPLC, and the column was Acquity BEH C18 (50 mm × 2.1 mm, 1.7 µm). The mobile phase consisted of solvent A which was 0.1% formic acid in water and solvent B which was ACN. The injected volume was 10 µl with a flow rate of 0.4 ml/min. samples were eluted following gradient program over 5 min. The column temperature was maintained at 35 °C. The detection was performed using Xevo TQD MS with ESI in positive mode. The method was validated according to ICH guidelines. Result: The results showed that the retention times of AMLO and PER were 1.65 min and 1.78 min respectively. Method was linear for AMLO and PER over the range of 5-15 ng/ml with R 2 of 0.9988 and 0.9991 respectively. The accuracies of AMLO and PER were in the range of 98.1% -100.19%. LOD and LOQ were 50.28 and 152.78 pg/ml for AMLO respectively and 16.86 and 51.1 pg/ml for PER respectively. The developed and validated method was applied for the quantification of AMLO and PER at tablet dosage form which is available at the market with satisfactory results. Conclusion: It can be concluded that this method is highly sensitive, rapid, selective and it can be used for the routine analysis of AMLO and PER in QC laboratories. 2018-08-24 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/68161/1/IPRC-2018-2.pdf Alaama, Mohamed and Helaluddin, Abul Bashar Mohammed and Awang, Mohamed and Abbas, Syed Atif (2018) Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form. In: 4th Pharmaceutical Research Conference 2018, 24th-26th August, 2018, Cyberjaya. (Unpublished) |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry |
| spellingShingle |
QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Alaama, Mohamed Helaluddin, Abul Bashar Mohammed Awang, Mohamed Abbas, Syed Atif Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form |
| description |
Introduction: Amlodipine (AMLO) and Perindopril (PER) have
been combined in one tablet dosage form which has been
proven to be an effective and well tolerated antihypertensive.
Many analytical methods were applied for the analysis of AMLO
and PER in this combination but most of the methods are time
and money consuming. A new sensitive, rapid, and specific LC-
MS/MS analytical methods for the analysis of AMLO and PER
was developed and validated. Chromatographic conditions for
the best results were optimized using design of experiment
method.
4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018
Challenging The Inquisitive Minds: Frontier Of The Future
24 – 26 August 2018 | Cyberjaya, Malaysia
Method: The instrument used was Waters Aquity UPLC, and
the column was Acquity BEH C18 (50 mm × 2.1 mm, 1.7 µm).
The mobile phase consisted of solvent A which was 0.1% formic
acid in water and solvent B which was ACN. The injected
volume was 10 µl with a flow rate of 0.4 ml/min. samples were
eluted following gradient program over 5 min. The column
temperature was maintained at 35 °C. The detection was
performed using Xevo TQD MS with ESI in positive mode. The
method was validated according to ICH guidelines.
Result: The results showed that the retention times of AMLO
and PER were 1.65 min and 1.78 min respectively. Method was
linear for AMLO and PER over the range of 5-15 ng/ml with R 2
of 0.9988 and 0.9991 respectively. The accuracies of AMLO and
PER were in the range of 98.1% -100.19%. LOD and LOQ were
50.28 and 152.78 pg/ml for AMLO respectively and 16.86 and
51.1 pg/ml for PER respectively. The developed and validated
method was applied for the quantification of AMLO and PER at
tablet dosage form which is available at the market with
satisfactory results.
Conclusion: It can be concluded that this method is highly
sensitive, rapid, selective and it can be used for the routine
analysis of AMLO and PER in QC laboratories. |
| format |
Conference or Workshop Item |
| author |
Alaama, Mohamed Helaluddin, Abul Bashar Mohammed Awang, Mohamed Abbas, Syed Atif |
| author_facet |
Alaama, Mohamed Helaluddin, Abul Bashar Mohammed Awang, Mohamed Abbas, Syed Atif |
| author_sort |
Alaama, Mohamed |
| title |
Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form |
| title_short |
Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form |
| title_full |
Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form |
| title_fullStr |
Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form |
| title_full_unstemmed |
Development and validation of LC-MSMS method for the analysis of amlodipine and perindopril in tablet dosage form |
| title_sort |
development and validation of lc-msms method for the analysis of amlodipine and perindopril in tablet dosage form |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/68161/ http://irep.iium.edu.my/68161/1/IPRC-2018-2.pdf |
| first_indexed |
2023-09-18T21:36:44Z |
| last_indexed |
2023-09-18T21:36:44Z |
| _version_ |
1777412871100039168 |