Growth kinetics of citrus Suhuiensis cell suspension cultures

Citrus is one of the major commodities in many countries including Malaysia. However, production of citrus including Citrus suhuiensis (C. suhuiensis) is declining due to diseases and inability to withstand low temperatures. Plant cultures such as cell suspension have the potential in propagating di...

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Main Authors:	Mohamad Puad, Noor Illi, Sarji, Muhammad Alif, M. Fathil, Nur Alia, Abduh, Muhammad Yusuf
Format:	Article
Language:	English
Published:	IIUM Press, International Islamic University Malaysia 2018
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/68301/ http://irep.iium.edu.my/68301/ http://irep.iium.edu.my/68301/1/68301_GROWTH%20KINETICS%20OF%20Citrus%20suhuiensis.pdf

id	iium-68301
recordtype	eprints
spelling	iium-683012019-07-11T02:06:50Z http://irep.iium.edu.my/68301/ Growth kinetics of citrus Suhuiensis cell suspension cultures Mohamad Puad, Noor Illi Sarji, Muhammad Alif M. Fathil, Nur Alia Abduh, Muhammad Yusuf T Technology (General) Citrus is one of the major commodities in many countries including Malaysia. However, production of citrus including Citrus suhuiensis (C. suhuiensis) is declining due to diseases and inability to withstand low temperatures. Plant cultures such as cell suspension have the potential in propagating disease-free and healthy Citrus fruits with value-added characteristics. However, studies related to C. suhuiensis is still scarce. Therefore, the growth kinetics of C. suhuiensis cell suspension culture was studied. Friable callus of C. suhuiensis which was induced from seeds was inoculated into MS medium with 30 g/L sucrose, 0.5 g/L malt extract and 2.0 mg/L 2, 4-D for the cell suspension initiation. Several batch experiments using a few types of sugars (sucrose, glucose and fructose) were carried out. The cell dry weight (CDW) of C. suhuiensis was recorded for 30 days of culture period and residual sugars in the medium were analyzed using HPLC. Cells grown in 30 g/L sucrose achieved the highest CDW (9.559 g/L) with μmax equals to 0.00512/h, compared to glucose and fructose. In addition, sucrose is the preferred carbon source with the highest uptake rate (0.213 g/L·h). Cells completely hydrolyzed sucrose into glucose and fructose after 5 days of inoculation. All sugars were completely utilized by C. suhuiensis cells after 25 days. The kinetic growth parameters determined from batch experiments were then used for model simulation and verification in MATHCAD 15. After adjustments and refinement to the selected kinetic parameters, the model has fairly described and predicted the growth and sugars profile of C. suhuiensis cells. The proposed model can be used to predict sucrose hydrolysis, glucose and fructose formation from sucrose and their consumption by plant cells and also for larger scale of growth. IIUM Press, International Islamic University Malaysia 2018-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68301/1/68301_GROWTH%20KINETICS%20OF%20Citrus%20suhuiensis.pdf Mohamad Puad, Noor Illi and Sarji, Muhammad Alif and M. Fathil, Nur Alia and Abduh, Muhammad Yusuf (2018) Growth kinetics of citrus Suhuiensis cell suspension cultures. Biological and Natural Resources Engineering Journal, 1 (1). pp. 1-13. ISSN 2637-0719 http://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/10
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Sarji, Muhammad Alif M. Fathil, Nur Alia Abduh, Muhammad Yusuf Growth kinetics of citrus Suhuiensis cell suspension cultures
description	Citrus is one of the major commodities in many countries including Malaysia. However, production of citrus including Citrus suhuiensis (C. suhuiensis) is declining due to diseases and inability to withstand low temperatures. Plant cultures such as cell suspension have the potential in propagating disease-free and healthy Citrus fruits with value-added characteristics. However, studies related to C. suhuiensis is still scarce. Therefore, the growth kinetics of C. suhuiensis cell suspension culture was studied. Friable callus of C. suhuiensis which was induced from seeds was inoculated into MS medium with 30 g/L sucrose, 0.5 g/L malt extract and 2.0 mg/L 2, 4-D for the cell suspension initiation. Several batch experiments using a few types of sugars (sucrose, glucose and fructose) were carried out. The cell dry weight (CDW) of C. suhuiensis was recorded for 30 days of culture period and residual sugars in the medium were analyzed using HPLC. Cells grown in 30 g/L sucrose achieved the highest CDW (9.559 g/L) with μmax equals to 0.00512/h, compared to glucose and fructose. In addition, sucrose is the preferred carbon source with the highest uptake rate (0.213 g/L·h). Cells completely hydrolyzed sucrose into glucose and fructose after 5 days of inoculation. All sugars were completely utilized by C. suhuiensis cells after 25 days. The kinetic growth parameters determined from batch experiments were then used for model simulation and verification in MATHCAD 15. After adjustments and refinement to the selected kinetic parameters, the model has fairly described and predicted the growth and sugars profile of C. suhuiensis cells. The proposed model can be used to predict sucrose hydrolysis, glucose and fructose formation from sucrose and their consumption by plant cells and also for larger scale of growth.
format	Article
author	Mohamad Puad, Noor Illi Sarji, Muhammad Alif M. Fathil, Nur Alia Abduh, Muhammad Yusuf
author_facet	Mohamad Puad, Noor Illi Sarji, Muhammad Alif M. Fathil, Nur Alia Abduh, Muhammad Yusuf
author_sort	Mohamad Puad, Noor Illi
title	Growth kinetics of citrus Suhuiensis cell suspension cultures
title_short	Growth kinetics of citrus Suhuiensis cell suspension cultures
title_full	Growth kinetics of citrus Suhuiensis cell suspension cultures
title_fullStr	Growth kinetics of citrus Suhuiensis cell suspension cultures
title_full_unstemmed	Growth kinetics of citrus Suhuiensis cell suspension cultures
title_sort	growth kinetics of citrus suhuiensis cell suspension cultures
publisher	IIUM Press, International Islamic University Malaysia
publishDate	2018
url	http://irep.iium.edu.my/68301/ http://irep.iium.edu.my/68301/ http://irep.iium.edu.my/68301/1/68301_GROWTH%20KINETICS%20OF%20Citrus%20suhuiensis.pdf
first_indexed	2023-09-18T21:36:56Z
last_indexed	2023-09-18T21:36:56Z
_version_	1777412882889179136

Growth kinetics of citrus Suhuiensis cell suspension cultures

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