Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry

Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose l...

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Main Authors:	Ramli, Nurul Izzati, Abd-Wahab, Firdaus, Wan Salim, Wan Wardatul Amani
Format:	Conference or Workshop Item
Language:	English English
Published:	AIP 2018
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf

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recordtype	eprints
spelling	iium-684432018-12-17T06:18:37Z http://irep.iium.edu.my/68443/ Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani TP155 Chemical engineering TP248.13 Biotechnology Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring. AIP 2018-11-29 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf application/pdf en http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf Ramli, Nurul Izzati and Abd-Wahab, Firdaus and Wan Salim, Wan Wardatul Amani (2018) Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry. In: 3rd International Sciences, Technology & Engineering Conference (ISTEC) 2018 - Material Chemistry, 18th April 2018, Penang, Malaysia. https://aip.scitation.org/doi/10.1063/1.5066993 10.1063/1.5066993
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
description	Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring.
format	Conference or Workshop Item
author	Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani
author_facet	Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani
author_sort	Ramli, Nurul Izzati
title	Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_short	Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_full	Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_fullStr	Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_full_unstemmed	Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_sort	characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
publisher	AIP
publishDate	2018
url	http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf
first_indexed	2023-09-18T21:37:09Z
last_indexed	2023-09-18T21:37:09Z
_version_	1777412896477675520

Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry

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