Screening fungi For lipid production For consolidated bioprocessing

This paper discusses about the production of lipid and glucose by three different fungi; Aspergillus niger, Aspergillus Oryzae, and Rhizopus sp.. Biodiesel consisting of methyl esters can be produced from fungal-based lipids through transesterification. Biodiesel can be produced from various carbon...

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Main Authors:	Hasni, Mohd Haffizi, Ahmad, Farah, Athoillah, Ahdyat Zain, Mansor, Mariatul Fadzillah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering International Islamic University Malaysia (IIUM) 50728 Kuala Lumpur Malaysia 2018
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol TP670 Oils, fats, waxes
Online Access:	http://irep.iium.edu.my/68528/ http://irep.iium.edu.my/68528/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf

id	iium-68528
recordtype	eprints
spelling	iium-685282019-07-03T07:24:59Z http://irep.iium.edu.my/68528/ Screening fungi For lipid production For consolidated bioprocessing Hasni, Mohd Haffizi Ahmad, Farah Athoillah, Ahdyat Zain Mansor, Mariatul Fadzillah TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol TP670 Oils, fats, waxes This paper discusses about the production of lipid and glucose by three different fungi; Aspergillus niger, Aspergillus Oryzae, and Rhizopus sp.. Biodiesel consisting of methyl esters can be produced from fungal-based lipids through transesterification. Biodiesel can be produced from various carbon substrates. However, the existing production of biodiesel from lignocellulosic biomass is not economically feasible due to the multiple processes required; (1) pre-treatment process to break down complex structure of lignin-hemicellulose-cellulose matrix, (2) saccharification of cellulose via cellulases and xylanases into simple sugars, and (3) fermentation of simple sugars. Consolidated bioprocess (CBP) combines saccharification and fermentation into one reactor. A single microorganism needs to be able to produce cellulase, break down cellulose to produce simple sugars, and ferment it to produce lipid in CBP. The objective of this study is to find the most promising fungi amongst the studied strains to be used in CBP by investigating the fungi’s ability to produce lipid. M9 medium supplemented with carboxymethylcellulose was used for glucose production and was incubated for 6 days. Basic media was used for lipid production and Bligh and Dyer lipid extraction method was used to extract lipid from fungi. The results showed that Rhizopus sp. has the potential to be promising microorganisms for CBP as it has the highest lipid productivity and lowest standard deviation of glucose production in comparison to the other fungi. Although it did not produce the highest glucose concentration, this may be solved in subsequent experiment by media optimization. Kulliyyah of Engineering International Islamic University Malaysia (IIUM) 50728 Kuala Lumpur Malaysia 2018-09 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/68528/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf Hasni, Mohd Haffizi and Ahmad, Farah and Athoillah, Ahdyat Zain and Mansor, Mariatul Fadzillah (2018) Screening fungi For lipid production For consolidated bioprocessing. In: 5th International Conference Biotechnology Engineering (ICBioE ‘18), 19 - 20 September 2018, Gombak, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol TP670 Oils, fats, waxes
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol TP670 Oils, fats, waxes Hasni, Mohd Haffizi Ahmad, Farah Athoillah, Ahdyat Zain Mansor, Mariatul Fadzillah Screening fungi For lipid production For consolidated bioprocessing
description	This paper discusses about the production of lipid and glucose by three different fungi; Aspergillus niger, Aspergillus Oryzae, and Rhizopus sp.. Biodiesel consisting of methyl esters can be produced from fungal-based lipids through transesterification. Biodiesel can be produced from various carbon substrates. However, the existing production of biodiesel from lignocellulosic biomass is not economically feasible due to the multiple processes required; (1) pre-treatment process to break down complex structure of lignin-hemicellulose-cellulose matrix, (2) saccharification of cellulose via cellulases and xylanases into simple sugars, and (3) fermentation of simple sugars. Consolidated bioprocess (CBP) combines saccharification and fermentation into one reactor. A single microorganism needs to be able to produce cellulase, break down cellulose to produce simple sugars, and ferment it to produce lipid in CBP. The objective of this study is to find the most promising fungi amongst the studied strains to be used in CBP by investigating the fungi’s ability to produce lipid. M9 medium supplemented with carboxymethylcellulose was used for glucose production and was incubated for 6 days. Basic media was used for lipid production and Bligh and Dyer lipid extraction method was used to extract lipid from fungi. The results showed that Rhizopus sp. has the potential to be promising microorganisms for CBP as it has the highest lipid productivity and lowest standard deviation of glucose production in comparison to the other fungi. Although it did not produce the highest glucose concentration, this may be solved in subsequent experiment by media optimization.
format	Conference or Workshop Item
author	Hasni, Mohd Haffizi Ahmad, Farah Athoillah, Ahdyat Zain Mansor, Mariatul Fadzillah
author_facet	Hasni, Mohd Haffizi Ahmad, Farah Athoillah, Ahdyat Zain Mansor, Mariatul Fadzillah
author_sort	Hasni, Mohd Haffizi
title	Screening fungi For lipid production For consolidated bioprocessing
title_short	Screening fungi For lipid production For consolidated bioprocessing
title_full	Screening fungi For lipid production For consolidated bioprocessing
title_fullStr	Screening fungi For lipid production For consolidated bioprocessing
title_full_unstemmed	Screening fungi For lipid production For consolidated bioprocessing
title_sort	screening fungi for lipid production for consolidated bioprocessing
publisher	Kulliyyah of Engineering International Islamic University Malaysia (IIUM) 50728 Kuala Lumpur Malaysia
publishDate	2018
url	http://irep.iium.edu.my/68528/ http://irep.iium.edu.my/68528/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf
first_indexed	2023-09-18T21:37:15Z
last_indexed	2023-09-18T21:37:15Z
_version_	1777412902878183424

Screening fungi For lipid production For consolidated bioprocessing

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