Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation

Microbial biosurfactants are amphiphilic, surface-active compounds produced during biodegradation, increasing the bioavailability of organic pollutants such as hydrocarbons. This study aimed to determine the association between the ability of indigenous bacteria isolated from petroleum contaminated...

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Main Authors:	Azmi, Siti Norzilah, Mohd Shahrom, Amalia Sakina, Razali, Muhammad Aizat, Mohd Ashaari, Mardiana
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/68537/ http://irep.iium.edu.my/68537/1/MSM2018poster_Mardiana.pdf http://irep.iium.edu.my/68537/7/MSM2018%20Book%20abstract.pdf

id	iium-68537
recordtype	eprints
spelling	iium-685372019-02-11T07:58:05Z http://irep.iium.edu.my/68537/ Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation Azmi, Siti Norzilah Mohd Shahrom, Amalia Sakina Razali, Muhammad Aizat Mohd Ashaari, Mardiana QR Microbiology Microbial biosurfactants are amphiphilic, surface-active compounds produced during biodegradation, increasing the bioavailability of organic pollutants such as hydrocarbons. This study aimed to determine the association between the ability of indigenous bacteria isolated from petroleum contaminated soil to produce biosurfactants with their ability to degrade total petroleum hydrocarbons (TPH). Four of these bacterial isolates (BSP1, BSP2, BSP3 and BSP4) were screened and characterized in terms of their potential in producing biosurfactants through qualitative and quantitative analysis; also supported by genetic characterization of the isolates by using molecular approach. These isolates exhibited some biosurfactant activities based on some qualitative (i.e emulsification assay) and quantitative screenings (i.e surface tension measurement). Highest emulsification activity exhibited by BSP4 (≈ 40 %) while for the rest of the isolates were between 22 to 27 %. Surface tension measurement confirmed the biosurfactant activity of the isolates, which were as low as ≈ 43 mN/m for BSP4 and BSP3. On the other hand, TPH biodegradation study was conducted for these four using GC-MS. Assuming growth-linked biodegradation; growth curves of these bacterial isolates showed the highest optical density reading after 36 hours of incubation and therefore suggesting rapid metabolism of hydrocarbons by the isolates within this timeframe. However, after seven days, biodegradation percentage of TPH showed the vice versa of the results for biosurfactant activities; BSP4 had the lowest TPH biodegradation percentage (≈ 28 %) although being the most potent in lowering surface tension. Meanwhile, BSP1 had the highest TPH biodegradation percentage (≈ 63 %) despite having the least biosurfactant activity measurements. Confirmation by genetic characterization revealed that isolates BSP3 had various genes encoding for biosurfactant synthesis (fenD, ituC and rhlAB) but none of the isolates had the ring-hydroxylating dioxygenase gene responsible in the initial step of TPH biodegradation. In conclusion, association of biosurfactant produced by indigenous bacteria isolated from petroleum contaminated soil with TPH biodegradation cannot be determined in this study. However, biosurfactants produced could be extracted and purified to be used in many industries and applications. 2018-12-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/68537/1/MSM2018poster_Mardiana.pdf application/pdf en http://irep.iium.edu.my/68537/7/MSM2018%20Book%20abstract.pdf Azmi, Siti Norzilah and Mohd Shahrom, Amalia Sakina and Razali, Muhammad Aizat and Mohd Ashaari, Mardiana (2018) Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation. In: 34th Symposium of The Malaysian Society for Microbiology 2018, 7th-10th December 2018, The Gurney Hotel and Residence, Georgetown, Penang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Azmi, Siti Norzilah Mohd Shahrom, Amalia Sakina Razali, Muhammad Aizat Mohd Ashaari, Mardiana Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
description	Microbial biosurfactants are amphiphilic, surface-active compounds produced during biodegradation, increasing the bioavailability of organic pollutants such as hydrocarbons. This study aimed to determine the association between the ability of indigenous bacteria isolated from petroleum contaminated soil to produce biosurfactants with their ability to degrade total petroleum hydrocarbons (TPH). Four of these bacterial isolates (BSP1, BSP2, BSP3 and BSP4) were screened and characterized in terms of their potential in producing biosurfactants through qualitative and quantitative analysis; also supported by genetic characterization of the isolates by using molecular approach. These isolates exhibited some biosurfactant activities based on some qualitative (i.e emulsification assay) and quantitative screenings (i.e surface tension measurement). Highest emulsification activity exhibited by BSP4 (≈ 40 %) while for the rest of the isolates were between 22 to 27 %. Surface tension measurement confirmed the biosurfactant activity of the isolates, which were as low as ≈ 43 mN/m for BSP4 and BSP3. On the other hand, TPH biodegradation study was conducted for these four using GC-MS. Assuming growth-linked biodegradation; growth curves of these bacterial isolates showed the highest optical density reading after 36 hours of incubation and therefore suggesting rapid metabolism of hydrocarbons by the isolates within this timeframe. However, after seven days, biodegradation percentage of TPH showed the vice versa of the results for biosurfactant activities; BSP4 had the lowest TPH biodegradation percentage (≈ 28 %) although being the most potent in lowering surface tension. Meanwhile, BSP1 had the highest TPH biodegradation percentage (≈ 63 %) despite having the least biosurfactant activity measurements. Confirmation by genetic characterization revealed that isolates BSP3 had various genes encoding for biosurfactant synthesis (fenD, ituC and rhlAB) but none of the isolates had the ring-hydroxylating dioxygenase gene responsible in the initial step of TPH biodegradation. In conclusion, association of biosurfactant produced by indigenous bacteria isolated from petroleum contaminated soil with TPH biodegradation cannot be determined in this study. However, biosurfactants produced could be extracted and purified to be used in many industries and applications.
format	Conference or Workshop Item
author	Azmi, Siti Norzilah Mohd Shahrom, Amalia Sakina Razali, Muhammad Aizat Mohd Ashaari, Mardiana
author_facet	Azmi, Siti Norzilah Mohd Shahrom, Amalia Sakina Razali, Muhammad Aizat Mohd Ashaari, Mardiana
author_sort	Azmi, Siti Norzilah
title	Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
title_short	Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
title_full	Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
title_fullStr	Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
title_full_unstemmed	Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
title_sort	association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation
publishDate	2018
url	http://irep.iium.edu.my/68537/ http://irep.iium.edu.my/68537/1/MSM2018poster_Mardiana.pdf http://irep.iium.edu.my/68537/7/MSM2018%20Book%20abstract.pdf
first_indexed	2023-09-18T21:37:16Z
last_indexed	2023-09-18T21:37:16Z
_version_	1777412904067268608

Association of biosurfactant production by indigenous bacteria isolated from petroleum contaminated soil with total petroleum hydrocarbon degradation

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