ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads

Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic...

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Main Authors:	Alkhatib, Hamzeh, Mohamed, Farahidah, Doolaanea, Abd Almonem
Format:	Article
Language:	English English
Published:	2018
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/1/ATR-FTIR%20AND%20SPECTROSCOPIC%20METHODS%20FOR%20ANALYSIS%20OF%20BLACK%20SEED%20OIL.pdf http://irep.iium.edu.my/68932/7/Scopus%20-%20ATR-FTIR.pdf

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spelling	iium-689322018-12-26T07:34:25Z http://irep.iium.edu.my/68932/ ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem RS192 Materia Medica-Pharmaceutical Technology Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer. Results: ATR-FTIR method exhibited linearity in the range of 25-300 mg BSO /ml emulsion (mg BSO /ml E )with R 2 =0.998, RSD=8.4%, LOD=0.28 mg BSO /ml E and LOQ=0.87 mg BSO /ml E . BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mg BSO /ml E , R 2 =0.9931, RSD=1.34%, LOD=0.89 mg BSO /ml E, and LOQ=2.71 mg BSO /ml E . In addition, the method showed that total amount of BSO was released at 110 min. Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology. 2018-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68932/1/ATR-FTIR%20AND%20SPECTROSCOPIC%20METHODS%20FOR%20ANALYSIS%20OF%20BLACK%20SEED%20OIL.pdf application/pdf en http://irep.iium.edu.my/68932/7/Scopus%20-%20ATR-FTIR.pdf Alkhatib, Hamzeh and Mohamed, Farahidah and Doolaanea, Abd Almonem (2018) ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads. International Journal of Applied Pharmaceutics, 10 (5). pp. 147-152. ISSN 0975-7058 https://innovareacademics.in/journals/index.php/ijap/article/view/25057/15688 10.22159/ijap.2018v10i5.25057
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
description	Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer. Results: ATR-FTIR method exhibited linearity in the range of 25-300 mg BSO /ml emulsion (mg BSO /ml E )with R 2 =0.998, RSD=8.4%, LOD=0.28 mg BSO /ml E and LOQ=0.87 mg BSO /ml E . BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mg BSO /ml E , R 2 =0.9931, RSD=1.34%, LOD=0.89 mg BSO /ml E, and LOQ=2.71 mg BSO /ml E . In addition, the method showed that total amount of BSO was released at 110 min. Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology.
format	Article
author	Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem
author_facet	Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem
author_sort	Alkhatib, Hamzeh
title	ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
title_short	ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
title_full	ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
title_fullStr	ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
title_full_unstemmed	ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
title_sort	atr-ftir and spectroscopic methods for analysis of black seed oil from alginate beads
publishDate	2018
url	http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/1/ATR-FTIR%20AND%20SPECTROSCOPIC%20METHODS%20FOR%20ANALYSIS%20OF%20BLACK%20SEED%20OIL.pdf http://irep.iium.edu.my/68932/7/Scopus%20-%20ATR-FTIR.pdf
first_indexed	2023-09-18T21:37:49Z
last_indexed	2023-09-18T21:37:49Z
_version_	1777412939327733760

ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads

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