Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group

An active microfibrous substrate containing aminated brush obtained by radiation-induced grafting of glycidyl methacrylate (GMA) onto a polyethylene/polypropylene (PE/PP) microfibrous sheet followed by amination reaction was prepared and used for immobilisation of Candida rugosa lipase under various...

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Main Authors:	Akbar Tajudin, Hazimah, Al-Khatib, Ma An Fahmi Rashid, Mirghani, Mohamed Elwathig Saeed, Mohd. Salleh, Hamzah, Nasef, Mohamed Mahmoud
Format:	Article
Language:	English English
Published:	The Academy of Sciences Malaysia 2018
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/69248/ http://irep.iium.edu.my/69248/ http://irep.iium.edu.my/69248/1/69248_Immobilisation%20of%20Candida%20rugosa%20Lipase.pdf http://irep.iium.edu.my/69248/7/69248_Immobilisation%20of%20Candida%20rugosa%20lipase%20on%20aminated%20polyethylene_SCOPUS.pdf

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recordtype	eprints
spelling	iium-692482019-02-21T07:37:02Z http://irep.iium.edu.my/69248/ Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group Akbar Tajudin, Hazimah Al-Khatib, Ma An Fahmi Rashid Mirghani, Mohamed Elwathig Saeed Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud TP155 Chemical engineering TP248.13 Biotechnology An active microfibrous substrate containing aminated brush obtained by radiation-induced grafting of glycidyl methacrylate (GMA) onto a polyethylene/polypropylene (PE/PP) microfibrous sheet followed by amination reaction was prepared and used for immobilisation of Candida rugosa lipase under various conditions. The aminated microfibrous sheet was characterised by Fourier-transform infrared spectroscopy (FTIR-ATR) and field emission scanning electron microscope (FESEM). The amine group density on the aminated micro-fibrous sheet was found to be 3.33mmol/g. Response surface methodology (RSM) was applied to model and optimise the immobilisation conditions including immobilisation time (2-6 h), medium pH (pH 7-9) and enzyme/support ratio (5.0-9.0mg/cm2). The model generated from RSM was significantly correlated with the studied parameters for the residual activity of the immobilised lipase. The optimum values for immobilisation time, medium pH, and enzyme/support ratio were found to be 4.24h, pH 8, and 8.51mg/cm2 respectively. The enzymatic activity using p-nitrophenyl palmitate (pNPP) as substrate was 1.4588U/cm2 under optimum conditions. The pH endurance, storage, and thermal stability of the immobilised lipase were remarkably enhanced. The immobilised lipase can be readily recovered and more than 50% of its activity was retained following 10 cycles. The results of this study suggested that the aminated microfibrous sheet of PE/PP grafted with poly(GMA) is a promising polymer support for enzyme immobilisation with high potential for broad biocatalytic applications. The Academy of Sciences Malaysia 2018-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69248/1/69248_Immobilisation%20of%20Candida%20rugosa%20Lipase.pdf application/pdf en http://irep.iium.edu.my/69248/7/69248_Immobilisation%20of%20Candida%20rugosa%20lipase%20on%20aminated%20polyethylene_SCOPUS.pdf Akbar Tajudin, Hazimah and Al-Khatib, Ma An Fahmi Rashid and Mirghani, Mohamed Elwathig Saeed and Mohd. Salleh, Hamzah and Nasef, Mohamed Mahmoud (2018) Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group. ASM Science Journal, 11 (3). pp. 182-200. E-ISSN 1823-6782 https://www.akademisains.gov.my/asmsj/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Akbar Tajudin, Hazimah Al-Khatib, Ma An Fahmi Rashid Mirghani, Mohamed Elwathig Saeed Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
description	An active microfibrous substrate containing aminated brush obtained by radiation-induced grafting of glycidyl methacrylate (GMA) onto a polyethylene/polypropylene (PE/PP) microfibrous sheet followed by amination reaction was prepared and used for immobilisation of Candida rugosa lipase under various conditions. The aminated microfibrous sheet was characterised by Fourier-transform infrared spectroscopy (FTIR-ATR) and field emission scanning electron microscope (FESEM). The amine group density on the aminated micro-fibrous sheet was found to be 3.33mmol/g. Response surface methodology (RSM) was applied to model and optimise the immobilisation conditions including immobilisation time (2-6 h), medium pH (pH 7-9) and enzyme/support ratio (5.0-9.0mg/cm2). The model generated from RSM was significantly correlated with the studied parameters for the residual activity of the immobilised lipase. The optimum values for immobilisation time, medium pH, and enzyme/support ratio were found to be 4.24h, pH 8, and 8.51mg/cm2 respectively. The enzymatic activity using p-nitrophenyl palmitate (pNPP) as substrate was 1.4588U/cm2 under optimum conditions. The pH endurance, storage, and thermal stability of the immobilised lipase were remarkably enhanced. The immobilised lipase can be readily recovered and more than 50% of its activity was retained following 10 cycles. The results of this study suggested that the aminated microfibrous sheet of PE/PP grafted with poly(GMA) is a promising polymer support for enzyme immobilisation with high potential for broad biocatalytic applications.
format	Article
author	Akbar Tajudin, Hazimah Al-Khatib, Ma An Fahmi Rashid Mirghani, Mohamed Elwathig Saeed Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud
author_facet	Akbar Tajudin, Hazimah Al-Khatib, Ma An Fahmi Rashid Mirghani, Mohamed Elwathig Saeed Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud
author_sort	Akbar Tajudin, Hazimah
title	Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
title_short	Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
title_full	Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
title_fullStr	Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
title_full_unstemmed	Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
title_sort	immobilisation of candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group
publisher	The Academy of Sciences Malaysia
publishDate	2018
url	http://irep.iium.edu.my/69248/ http://irep.iium.edu.my/69248/ http://irep.iium.edu.my/69248/1/69248_Immobilisation%20of%20Candida%20rugosa%20Lipase.pdf http://irep.iium.edu.my/69248/7/69248_Immobilisation%20of%20Candida%20rugosa%20lipase%20on%20aminated%20polyethylene_SCOPUS.pdf
first_indexed	2023-09-18T21:38:17Z
last_indexed	2023-09-18T21:38:17Z
_version_	1777412968686813184

Immobilisation of Candida rugosa lipase on aminated polyethylene/polypropylene microfibrous sheet modifieded with oxirane group

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