Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability

Thousands of antibiotics have been found from different actinomycetes but there were less study recorded on antimicrobial activity of rare actinomycete especially Actinophytocola sp. K4-08. Studies showed that evaluation on the antimicrobial potential of members of this genus has not been attempted...

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Main Authors:	Mohd Zin, Nur Huda, Zainal Abidin, Zaima Azira, Abdul Malek, Nurfathiah, Azizan, Nur Hafizah, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/69437/ http://irep.iium.edu.my/69437/1/POSTER%20MSM2018.pdf http://irep.iium.edu.my/69437/13/69437%20Programme%20book.pdf

id	iium-69437
recordtype	eprints
spelling	iium-694372019-01-23T00:29:49Z http://irep.iium.edu.my/69437/ Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability Mohd Zin, Nur Huda Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Azizan, Nur Hafizah Darnis, Deny Susanti QR Microbiology Thousands of antibiotics have been found from different actinomycetes but there were less study recorded on antimicrobial activity of rare actinomycete especially Actinophytocola sp. K4-08. Studies showed that evaluation on the antimicrobial potential of members of this genus has not been attempted so far, making it a prominent source of novel bioactive compounds.Therefore, the present study was carried out to isolate and identify antimicrobial activity of rare actinomycete, Actinophytocola sp. K4-08. Actinophytocola sp. K4-08 was successfully isolated from Kuantan mangrove sediment on starch-yeast extract agar (SYE) medium. Abundant cream colored substrate mycelium was formed on SYE and no obvious diffusible pigment was produced within 21 days of incubation. Partial 16S rRNA gene sequence revealed that Actinophytocola sp. K4-08 possessed 99% similarity to Actinophytocola sediminis YIM 75636. Moreover, both PKS-I and NRPS genes, both of which usually related to secondary metabolite potential were detected in this isolate through PCR amplification. Preliminary screening using cross streak test showed that Actinophytocola sp.K4-08 displayed antibacterial activity against B. subtilis and S. aureus. Its crude ethyl acetate extracts also displayed inhibitory activity against B. subtilis, further confirming the antimicrobial properties of this isolate. Actinophytocola sp. K4-08 was cultured in SYE broth followed by centrifugation to separate the cells and the supernatant. XAD-resin was added to the supernatant of 21-days culture broth followed by extraction with acetone. Crude ethyl acetate extract from the supernatant was also prepared and cells of Actinophytocola sp. K4-08 was extracted with methanol. All extracts (supernatant and cells) produced dark orange solid and yellow coloured oil. Disc diffusion assay against B. subtilis with concentration of 20, 40, 60, 80 and 100 µg/disc was conducted to check on the antibacterial capabilities of the extracts. All extracts (solid and oil) displayed weak inhibition activity against B. subtilis with oil possessed higher inhibition activity compared to the solid. Although the extracts produced weak antibacterial activity, nevertheless, the potential of rare actinomycete Actinophytocola sp. K4-08 indicated potential as bioactive compound producer that is worth exploring. Further investigation is needed in an attempt to isolate, purify and elucidate potential bioactive compounds produced by Actinophytocola sp. K4-08. 2018 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/69437/1/POSTER%20MSM2018.pdf application/pdf en http://irep.iium.edu.my/69437/13/69437%20Programme%20book.pdf Mohd Zin, Nur Huda and Zainal Abidin, Zaima Azira and Abdul Malek, Nurfathiah and Azizan, Nur Hafizah and Darnis, Deny Susanti (2018) Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability. In: 34th Symposium of the Malaysian Society for Microbiology 2018, 7th-10th December 2018, Penang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohd Zin, Nur Huda Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Azizan, Nur Hafizah Darnis, Deny Susanti Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability
description	Thousands of antibiotics have been found from different actinomycetes but there were less study recorded on antimicrobial activity of rare actinomycete especially Actinophytocola sp. K4-08. Studies showed that evaluation on the antimicrobial potential of members of this genus has not been attempted so far, making it a prominent source of novel bioactive compounds.Therefore, the present study was carried out to isolate and identify antimicrobial activity of rare actinomycete, Actinophytocola sp. K4-08. Actinophytocola sp. K4-08 was successfully isolated from Kuantan mangrove sediment on starch-yeast extract agar (SYE) medium. Abundant cream colored substrate mycelium was formed on SYE and no obvious diffusible pigment was produced within 21 days of incubation. Partial 16S rRNA gene sequence revealed that Actinophytocola sp. K4-08 possessed 99% similarity to Actinophytocola sediminis YIM 75636. Moreover, both PKS-I and NRPS genes, both of which usually related to secondary metabolite potential were detected in this isolate through PCR amplification. Preliminary screening using cross streak test showed that Actinophytocola sp.K4-08 displayed antibacterial activity against B. subtilis and S. aureus. Its crude ethyl acetate extracts also displayed inhibitory activity against B. subtilis, further confirming the antimicrobial properties of this isolate. Actinophytocola sp. K4-08 was cultured in SYE broth followed by centrifugation to separate the cells and the supernatant. XAD-resin was added to the supernatant of 21-days culture broth followed by extraction with acetone. Crude ethyl acetate extract from the supernatant was also prepared and cells of Actinophytocola sp. K4-08 was extracted with methanol. All extracts (supernatant and cells) produced dark orange solid and yellow coloured oil. Disc diffusion assay against B. subtilis with concentration of 20, 40, 60, 80 and 100 µg/disc was conducted to check on the antibacterial capabilities of the extracts. All extracts (solid and oil) displayed weak inhibition activity against B. subtilis with oil possessed higher inhibition activity compared to the solid. Although the extracts produced weak antibacterial activity, nevertheless, the potential of rare actinomycete Actinophytocola sp. K4-08 indicated potential as bioactive compound producer that is worth exploring. Further investigation is needed in an attempt to isolate, purify and elucidate potential bioactive compounds produced by Actinophytocola sp. K4-08.
format	Conference or Workshop Item
author	Mohd Zin, Nur Huda Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Azizan, Nur Hafizah Darnis, Deny Susanti
author_facet	Mohd Zin, Nur Huda Zainal Abidin, Zaima Azira Abdul Malek, Nurfathiah Azizan, Nur Hafizah Darnis, Deny Susanti
author_sort	Mohd Zin, Nur Huda
title	Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability
title_short	Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability
title_full	Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability
title_fullStr	Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability
title_full_unstemmed	Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability
title_sort	actinophytocola sp. k4-08: rare actinomycete with potential biosynthetic capability
publishDate	2018
url	http://irep.iium.edu.my/69437/ http://irep.iium.edu.my/69437/1/POSTER%20MSM2018.pdf http://irep.iium.edu.my/69437/13/69437%20Programme%20book.pdf
first_indexed	2023-09-18T21:38:33Z
last_indexed	2023-09-18T21:38:33Z
_version_	1777412985171476480

Actinophytocola sp. K4-08: rare actinomycete with potential biosynthetic capability

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