Study of cucumber mosaic virus gene expression in Capsicum annuum

Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major...

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Main Authors:	Azizan, Nur Hafizah, Zainal Abidin, Zulkifli Ahmad, Phang, Ing Chia
Format:	Article
Language:	English English
Published:	Zibeline International Publishing Sdn. Bhd. 2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/69664/ http://irep.iium.edu.my/69664/ http://irep.iium.edu.my/69664/1/study%20of%20cucumber%20mosaic%20virus%20gene%20experssion%20in%20capsicum%20annuum.pdf http://irep.iium.edu.my/69664/7/69664_Study%20of%20cucumber%20mosaic%20virus_googlescholar.pdf

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recordtype	eprints
spelling	iium-696642019-07-12T04:06:30Z http://irep.iium.edu.my/69664/ Study of cucumber mosaic virus gene expression in Capsicum annuum Azizan, Nur Hafizah Zainal Abidin, Zulkifli Ahmad Phang, Ing Chia Q Science (General) Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major diseases in Capsicum annum (chilli). Chilli plant samples exhibiting virus-like disease symptoms were collected from Taman Pertanian Indera Mahkota (location 1) and Greenhouse 12 of Horticulture Research Centre, Serdang (location 2). Viral disease was detected based on symptoms like mosaic-mottling, yellow ringspots and cholorotic that appeared on the leaves. The isolation of total RNA was done by using Vivantis GF-1 total RNA extraction kit. RT-PCR technique was used to detect the presence of virus disease symptoms gene in chillies. Identification of causal agents was based on cDNA amplified product size, using virus-specific oligonucleotides. Actin was used as the internal PCR control. The product size of the DNA fragment was 315 bp. From RT-PCR, the expression of CMV can be detected in chilli plants that exhibited the virus-like disease symptoms. This research revealed that some of the chilli plants at the Taman Pertanian Indera Mahkota and Greenhouse 12 of Horticulture Research Centre have been affected by this viral disease. Zibeline International Publishing Sdn. Bhd. 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69664/1/study%20of%20cucumber%20mosaic%20virus%20gene%20experssion%20in%20capsicum%20annuum.pdf application/pdf en http://irep.iium.edu.my/69664/7/69664_Study%20of%20cucumber%20mosaic%20virus_googlescholar.pdf Azizan, Nur Hafizah and Zainal Abidin, Zulkifli Ahmad and Phang, Ing Chia (2017) Study of cucumber mosaic virus gene expression in Capsicum annuum. Science Heritage Journal, 1 (2). pp. 29-31. ISSN 2521-0858 E-ISSN 2521-0866 https://jscienceheritage.com/archives/2gws2017/2gws2017-29-31.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Azizan, Nur Hafizah Zainal Abidin, Zulkifli Ahmad Phang, Ing Chia Study of cucumber mosaic virus gene expression in Capsicum annuum
description	Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major diseases in Capsicum annum (chilli). Chilli plant samples exhibiting virus-like disease symptoms were collected from Taman Pertanian Indera Mahkota (location 1) and Greenhouse 12 of Horticulture Research Centre, Serdang (location 2). Viral disease was detected based on symptoms like mosaic-mottling, yellow ringspots and cholorotic that appeared on the leaves. The isolation of total RNA was done by using Vivantis GF-1 total RNA extraction kit. RT-PCR technique was used to detect the presence of virus disease symptoms gene in chillies. Identification of causal agents was based on cDNA amplified product size, using virus-specific oligonucleotides. Actin was used as the internal PCR control. The product size of the DNA fragment was 315 bp. From RT-PCR, the expression of CMV can be detected in chilli plants that exhibited the virus-like disease symptoms. This research revealed that some of the chilli plants at the Taman Pertanian Indera Mahkota and Greenhouse 12 of Horticulture Research Centre have been affected by this viral disease.
format	Article
author	Azizan, Nur Hafizah Zainal Abidin, Zulkifli Ahmad Phang, Ing Chia
author_facet	Azizan, Nur Hafizah Zainal Abidin, Zulkifli Ahmad Phang, Ing Chia
author_sort	Azizan, Nur Hafizah
title	Study of cucumber mosaic virus gene expression in Capsicum annuum
title_short	Study of cucumber mosaic virus gene expression in Capsicum annuum
title_full	Study of cucumber mosaic virus gene expression in Capsicum annuum
title_fullStr	Study of cucumber mosaic virus gene expression in Capsicum annuum
title_full_unstemmed	Study of cucumber mosaic virus gene expression in Capsicum annuum
title_sort	study of cucumber mosaic virus gene expression in capsicum annuum
publisher	Zibeline International Publishing Sdn. Bhd.
publishDate	2017
url	http://irep.iium.edu.my/69664/ http://irep.iium.edu.my/69664/ http://irep.iium.edu.my/69664/1/study%20of%20cucumber%20mosaic%20virus%20gene%20experssion%20in%20capsicum%20annuum.pdf http://irep.iium.edu.my/69664/7/69664_Study%20of%20cucumber%20mosaic%20virus_googlescholar.pdf
first_indexed	2023-09-18T21:38:53Z
last_indexed	2023-09-18T21:38:53Z
_version_	1777413005563133952

Study of cucumber mosaic virus gene expression in Capsicum annuum

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