GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity

ABSTRACT Different extraction processes were employed to extract bioactive metabolites from Salacca zalacca flesh by a range of aqueous and organic solvents. The highest extraction yield was obtained by 50% ethanol extract of SE (73.18 ±4.35%), whereas SFE_1 showed the lowest yield (0.42 ±0.08%). A...

Full description

Bibliographic Details
Main Authors:	Saleh, Mohammed S M, Mohd Bukhari, Dzatil Awanis, Siddiqui, Mohammad Jamshed Ahmad, Kasmuri, Abdul Razak, Murugesu, Suganya, Khatib, Alfi
Format:	Article
Language:	English English
Published:	Taylor & Francis 2019
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/1/Natural%20Product%20Research%20_2019%20Salak.pdf http://irep.iium.edu.my/70206/7/70206_GC-MS%20analysis%20of%20metabolites%20from%20soxhlet%20extraction%2C%20ultrasound-assisted%20extraction%20and%20supercritical%20fluid%20extraction%20of%20Salacca_scopus.pdf

id	iium-70206
recordtype	eprints
spelling	iium-702062019-08-02T03:37:30Z http://irep.iium.edu.my/70206/ GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity Saleh, Mohammed S M Mohd Bukhari, Dzatil Awanis Siddiqui, Mohammad Jamshed Ahmad Kasmuri, Abdul Razak Murugesu, Suganya Khatib, Alfi QD Chemistry ABSTRACT Different extraction processes were employed to extract bioactive metabolites from Salacca zalacca flesh by a range of aqueous and organic solvents. The highest extraction yield was obtained by 50% ethanol extract of SE (73.18 ±4.35%), whereas SFE_1 showed the lowest yield (0.42 ±0.08%). All extracts were evaluated for in vitro a-glucosidase inhibitory activity, measured by their IC50 values in comparison to that of quercetin, the positive control (IC50 ¼ 2.7 ±0.7 lg/mL). The lowest a-glucosidase inhibitory activity was indicated by water extract of SE (IC50 ¼ 724.3 ±42.9 lg/mL) and the highest activity was demonstrated by 60% ethanol extract by UAE (IC50 ¼ 16.2 ± 2.4 lg/mL). All extracts were analysed by GC-MS and identified metabolites like carbohydrates, fatty acids, organic acids, phenolic acids, sterols and alkane-based compounds etcetera that may possess the potential as a-glucosidase inhibitor and may attribute to the a-glucosidase inhibitory activity. Taylor & Francis 2019-01-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70206/1/Natural%20Product%20Research%20_2019%20Salak.pdf application/pdf en http://irep.iium.edu.my/70206/7/70206_GC-MS%20analysis%20of%20metabolites%20from%20soxhlet%20extraction%2C%20ultrasound-assisted%20extraction%20and%20supercritical%20fluid%20extraction%20of%20Salacca_scopus.pdf Saleh, Mohammed S M and Mohd Bukhari, Dzatil Awanis and Siddiqui, Mohammad Jamshed Ahmad and Kasmuri, Abdul Razak and Murugesu, Suganya and Khatib, Alfi (2019) GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity. Natural Product Research. ISSN 1478-6419 E-ISSN 1478-6427 (In Press) https://www.tandfonline.com/loi/gnpl20 10.1080/14786419.2018.1560295
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Saleh, Mohammed S M Mohd Bukhari, Dzatil Awanis Siddiqui, Mohammad Jamshed Ahmad Kasmuri, Abdul Razak Murugesu, Suganya Khatib, Alfi GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
description	ABSTRACT Different extraction processes were employed to extract bioactive metabolites from Salacca zalacca flesh by a range of aqueous and organic solvents. The highest extraction yield was obtained by 50% ethanol extract of SE (73.18 ±4.35%), whereas SFE_1 showed the lowest yield (0.42 ±0.08%). All extracts were evaluated for in vitro a-glucosidase inhibitory activity, measured by their IC50 values in comparison to that of quercetin, the positive control (IC50 ¼ 2.7 ±0.7 lg/mL). The lowest a-glucosidase inhibitory activity was indicated by water extract of SE (IC50 ¼ 724.3 ±42.9 lg/mL) and the highest activity was demonstrated by 60% ethanol extract by UAE (IC50 ¼ 16.2 ± 2.4 lg/mL). All extracts were analysed by GC-MS and identified metabolites like carbohydrates, fatty acids, organic acids, phenolic acids, sterols and alkane-based compounds etcetera that may possess the potential as a-glucosidase inhibitor and may attribute to the a-glucosidase inhibitory activity.
format	Article
author	Saleh, Mohammed S M Mohd Bukhari, Dzatil Awanis Siddiqui, Mohammad Jamshed Ahmad Kasmuri, Abdul Razak Murugesu, Suganya Khatib, Alfi
author_facet	Saleh, Mohammed S M Mohd Bukhari, Dzatil Awanis Siddiqui, Mohammad Jamshed Ahmad Kasmuri, Abdul Razak Murugesu, Suganya Khatib, Alfi
author_sort	Saleh, Mohammed S M
title	GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
title_short	GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
title_full	GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
title_fullStr	GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
title_full_unstemmed	GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity
title_sort	gc-ms analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of salacca zalacca flesh and its alpha-glucosidase inhibitory activity
publisher	Taylor & Francis
publishDate	2019
url	http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/ http://irep.iium.edu.my/70206/1/Natural%20Product%20Research%20_2019%20Salak.pdf http://irep.iium.edu.my/70206/7/70206_GC-MS%20analysis%20of%20metabolites%20from%20soxhlet%20extraction%2C%20ultrasound-assisted%20extraction%20and%20supercritical%20fluid%20extraction%20of%20Salacca_scopus.pdf
first_indexed	2023-09-18T21:39:40Z
last_indexed	2023-09-18T21:39:40Z
_version_	1777413055069552640

GC-MS analysis of metabolites from soxhlet extraction, ultrasound-assisted extraction and supercritical fluid extraction of Salacca zalacca flesh and its alpha-glucosidase inhibitory activity

Similar Items