Site-directed mutagenesis on plasmid using polymerase chain reaction

Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid ha...

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Main Authors:	Ahmad, Abdul Aziz, Mohd. Salleh, Hamzah, Noorbatcha, Ibrahim Ali
Other Authors:	Amid, Azura
Format:	Book Chapter
Language:	English English
Published:	Springer Nature 2018
Subjects:	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf

id	iium-70243
recordtype	eprints
spelling	iium-702432020-03-05T10:19:05Z http://irep.iium.edu.my/70243/ Site-directed mutagenesis on plasmid using polymerase chain reaction Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali TP200 Manufacture and use of chemicals TP248.13 Biotechnology Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid harboring an endoglucanase I gene from Fusarium oxysporum is described here. Springer Nature Amid, Azura Sulaiman, Sarina Jimat, Dzun Noraini Mohamed Azmin, Nor Fadhillah 2018 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf application/pdf en http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf Ahmad, Abdul Aziz and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali (2018) Site-directed mutagenesis on plasmid using polymerase chain reaction. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 1-12. ISBN 978-981-13-2256-3 https://www.springer.com/us/book/9789811322563 https://doi.org/10.1007/978-981-13-2257-0
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
spellingShingle	TP200 Manufacture and use of chemicals TP248.13 Biotechnology Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Site-directed mutagenesis on plasmid using polymerase chain reaction
description	Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid harboring an endoglucanase I gene from Fusarium oxysporum is described here.
author2	Amid, Azura
author_facet	Amid, Azura Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali
format	Book Chapter
author	Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali
author_sort	Ahmad, Abdul Aziz
title	Site-directed mutagenesis on plasmid using polymerase chain reaction
title_short	Site-directed mutagenesis on plasmid using polymerase chain reaction
title_full	Site-directed mutagenesis on plasmid using polymerase chain reaction
title_fullStr	Site-directed mutagenesis on plasmid using polymerase chain reaction
title_full_unstemmed	Site-directed mutagenesis on plasmid using polymerase chain reaction
title_sort	site-directed mutagenesis on plasmid using polymerase chain reaction
publisher	Springer Nature
publishDate	2018
url	http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf
first_indexed	2023-09-18T21:39:43Z
last_indexed	2023-09-18T21:39:43Z
_version_	1777413058256175104

Site-directed mutagenesis on plasmid using polymerase chain reaction

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