Issues and challenges in the development of three-dimensional model for thyroid cancer

Issues and challenges in the development of three-dimensional model for thyroid cancer

Thyroid is the biggest gland in the body and in case of thyroid cancer, the biological characteristics and prognosis are completely different for different types of thyroid cancer. There are four types of thyroid cancer; papillary, follicular, medullary and anaplastic thyroid cancers. Among these ty...

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Main Author: Shahbuddin, Munira
Format: Conference or Workshop Item
Language:English
Published: Kulliyyah of Engineering, International Islamic University Malaysia 2018
Subjects:
TA164 Bioengineering
Online Access:http://irep.iium.edu.my/70371/
http://irep.iium.edu.my/70371/15/70371%20Issues%20and%20challenges%20with%203-dimensional%20thyroid%20cancer%20model.pdf
id iium-70371
recordtype eprints
spelling iium-703712019-03-08T01:31:10Z http://irep.iium.edu.my/70371/ Issues and challenges in the development of three-dimensional model for thyroid cancer Shahbuddin, Munira TA164 Bioengineering Thyroid is the biggest gland in the body and in case of thyroid cancer, the biological characteristics and prognosis are completely different for different types of thyroid cancer. There are four types of thyroid cancer; papillary, follicular, medullary and anaplastic thyroid cancers. Among these type, anaplastic carcinoma is the rarest and deadliest form of thyroid cancer, often due to poor diagnosis and rapid metastasis. Insignificant differences in preoperative tumor aggressiveness biomarker between each cancer type made slow progress in the development of drug and treatment for thyroid cancer. There have been substantial interest over the past years to develop a 3D human thyroid cancer model for investigation of new therapies. An accurate in vitro model of thyroid cancer with confirmation of the genetic drivers of the cancer will provide an optimal setting for preclinical testing and therapeutics. In this age of personalized medicine, this model can be effective at specializing treatments for the corresponding patients. However, there were reports that mRNA, cytokine and growth factor expression profiles from different type of thyroid cancer cell lines in vitro had shown similarity to the phenotypes of undifferentiated thyroid cancer cells in vivo. The phenotypic similarity of differentiated cell lines in vitro to undifferentiated cells in vivo raised questions on the accuracy and reliability of the spheroid, which is aggregation of cells in spherical form that widely used to mimic tumorigenesis of a cancer model. The use of hydrogel and additional extracellular matrices like fibrinogen and collagen embedded with spheroids and human umbilical vascular endothelial cells (HUVEC) in the 3D model is considered too simple to mimic the physiology and biochemistry of tumorigenesis. The needs for additional growth factors and chemokines in the supplementation affects many aspects of each cell behavior is able to modulate the outcome of the experiment and diagnosis for treatment. Herein, in this review, we would like to discuss in details appropriate in vitro and in vivo conditions of different type of thyroid cancer cells and its relation to physicochemical microenvironment. Despite the paucity of target expression and validation studies on this 3D vascularized model, it is very important that scientists incorporate the basis of cellular biology and materials science in the development of 3D model for therapeutic studies. This should be notable that any slight changes in cellular activities and microenvironment raising the risk of maturation phase in angiogenesis and may influence the outcomes of the treatment. Kulliyyah of Engineering, International Islamic University Malaysia 2018-09-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/70371/15/70371%20Issues%20and%20challenges%20with%203-dimensional%20thyroid%20cancer%20model.pdf Shahbuddin, Munira (2018) Issues and challenges in the development of three-dimensional model for thyroid cancer. In: International Conference Biotechnology Engineering, ICBioE ‘18 September 19-20, 2018,, 19th-20th September 2018, Kuala Lumpur, Malaysia.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TA164 Bioengineering
spellingShingle TA164 Bioengineering
Shahbuddin, Munira
Issues and challenges in the development of three-dimensional model for thyroid cancer
description Thyroid is the biggest gland in the body and in case of thyroid cancer, the biological characteristics and prognosis are completely different for different types of thyroid cancer. There are four types of thyroid cancer; papillary, follicular, medullary and anaplastic thyroid cancers. Among these type, anaplastic carcinoma is the rarest and deadliest form of thyroid cancer, often due to poor diagnosis and rapid metastasis. Insignificant differences in preoperative tumor aggressiveness biomarker between each cancer type made slow progress in the development of drug and treatment for thyroid cancer. There have been substantial interest over the past years to develop a 3D human thyroid cancer model for investigation of new therapies. An accurate in vitro model of thyroid cancer with confirmation of the genetic drivers of the cancer will provide an optimal setting for preclinical testing and therapeutics. In this age of personalized medicine, this model can be effective at specializing treatments for the corresponding patients. However, there were reports that mRNA, cytokine and growth factor expression profiles from different type of thyroid cancer cell lines in vitro had shown similarity to the phenotypes of undifferentiated thyroid cancer cells in vivo. The phenotypic similarity of differentiated cell lines in vitro to undifferentiated cells in vivo raised questions on the accuracy and reliability of the spheroid, which is aggregation of cells in spherical form that widely used to mimic tumorigenesis of a cancer model. The use of hydrogel and additional extracellular matrices like fibrinogen and collagen embedded with spheroids and human umbilical vascular endothelial cells (HUVEC) in the 3D model is considered too simple to mimic the physiology and biochemistry of tumorigenesis. The needs for additional growth factors and chemokines in the supplementation affects many aspects of each cell behavior is able to modulate the outcome of the experiment and diagnosis for treatment. Herein, in this review, we would like to discuss in details appropriate in vitro and in vivo conditions of different type of thyroid cancer cells and its relation to physicochemical microenvironment. Despite the paucity of target expression and validation studies on this 3D vascularized model, it is very important that scientists incorporate the basis of cellular biology and materials science in the development of 3D model for therapeutic studies. This should be notable that any slight changes in cellular activities and microenvironment raising the risk of maturation phase in angiogenesis and may influence the outcomes of the treatment.
format Conference or Workshop Item
author Shahbuddin, Munira
author_facet Shahbuddin, Munira
author_sort Shahbuddin, Munira
title Issues and challenges in the development of three-dimensional model for thyroid cancer
title_short Issues and challenges in the development of three-dimensional model for thyroid cancer
title_full Issues and challenges in the development of three-dimensional model for thyroid cancer
title_fullStr Issues and challenges in the development of three-dimensional model for thyroid cancer
title_full_unstemmed Issues and challenges in the development of three-dimensional model for thyroid cancer
title_sort issues and challenges in the development of three-dimensional model for thyroid cancer
publisher Kulliyyah of Engineering, International Islamic University Malaysia
publishDate 2018
url http://irep.iium.edu.my/70371/
http://irep.iium.edu.my/70371/15/70371%20Issues%20and%20challenges%20with%203-dimensional%20thyroid%20cancer%20model.pdf
first_indexed 2023-09-18T21:39:54Z
last_indexed 2023-09-18T21:39:54Z
_version_ 1777413070334722048

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