Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds

Biomaterial scaffolds play an important role in cartilage tissue engineering. They act as a temporary extracellular matrix substitute and template for cellular growth in vitro and support mechanical integrity in vivo. Several limitations have been reported when natural and synthetic biomaterials wer...

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Main Authors:	Mohamed Amin, Muhammad Azri Ifwat, Md Ali @ Tahir, Aisyah Hanani, Azhim, Azran, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English English English
Published:	Institute of Electrical and Electronics Engineers Inc. 2019
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/70448/ http://irep.iium.edu.my/70448/ http://irep.iium.edu.my/70448/ http://irep.iium.edu.my/70448/1/70448_Physical%20properties%20and%20biocompatibility.pdf http://irep.iium.edu.my/70448/2/70448_Physical%20properties%20and%20biocompatibility_SCOPUS.pdf http://irep.iium.edu.my/70448/3/70448_Physical%20properties%20and%20biocompatibility_WOS.pdf

id	iium-70448
recordtype	eprints
spelling	iium-704482019-06-13T08:10:04Z http://irep.iium.edu.my/70448/ Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds Mohamed Amin, Muhammad Azri Ifwat Md Ali @ Tahir, Aisyah Hanani Azhim, Azran Sha'ban, Munirah TA164 Bioengineering Biomaterial scaffolds play an important role in cartilage tissue engineering. They act as a temporary extracellular matrix substitute and template for cellular growth in vitro and support mechanical integrity in vivo. Several limitations have been reported when natural and synthetic biomaterials were used individually. They are unable to satisfactorily mimic the actual substrate for tissue regeneration both structurally and functionally. The formation of hybrid scaffolds consisting natural and synthetic biomaterials has been proposed to improve the outcomes. therefore, this study aimed to form and evaluate three-dimensional (3D) 65:35 poly(lactic-co-glycolic acid) PLGA (“P”) scaffold incorporated with and without atelocollagen (“A”) and/or fibrin (“F”). The PLGA scaffold was formed using solvent casting and salt leaching method, freeze-dried, and crosslinked with the natural biomaterials. The hybrid scaffolds groups were “PAF”, “PA” and “PF”. The PLGA only scaffold was used as control. All scaffolds were evaluated using selected analytical techniques namely Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy analysis, scanning electron microscopy (SEM), porosity study, water uptake capacity, and cytocompatibility analysis in “chondrocyte-scaffold” constructs groups at week 1, 2 and 3 in vitro. The interaction of PLGA scaffold with fibrin and/or atelocollagen was evidenced based on the notable peaks of amide bonds. The microscopic pore structures appeared interconnected. Total porosity in all scaffolds were varied. All groups demonstrated porosity range from 56% to 61%. Proper swelling ratio of the scaffold is crucial to mimic the water uptake capacity in the living tissues. Despite its hydrophobicity, the PLGA only group demonstrated the highest swelling ratio when compared to other groups though, the difference is not significant. The cytocompatibility of all scaffolds except PA showed similar trends of cell proliferation. In this study, the incorporation of fibrin facilitates better microenvironment for cellular growth in terms of cell adherence to the scaffolds compared to the groups without fibrin. Based on the preliminary results, the 65:35 PLGA based hybrid scaffolds may have the potential for cartilage tissue engineering application. Institute of Electrical and Electronics Engineers Inc. 2019 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/70448/1/70448_Physical%20properties%20and%20biocompatibility.pdf application/pdf en http://irep.iium.edu.my/70448/2/70448_Physical%20properties%20and%20biocompatibility_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/70448/3/70448_Physical%20properties%20and%20biocompatibility_WOS.pdf Mohamed Amin, Muhammad Azri Ifwat and Md Ali @ Tahir, Aisyah Hanani and Azhim, Azran and Sha'ban, Munirah (2019) Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds. In: 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences )IECBES 2018), 3rd-6th December 2018, Kuching, Sarawak. https://ieeexplore.ieee.org/document/8626673 10.1109/IECBES.2018.8626673
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Mohamed Amin, Muhammad Azri Ifwat Md Ali @ Tahir, Aisyah Hanani Azhim, Azran Sha'ban, Munirah Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
description	Biomaterial scaffolds play an important role in cartilage tissue engineering. They act as a temporary extracellular matrix substitute and template for cellular growth in vitro and support mechanical integrity in vivo. Several limitations have been reported when natural and synthetic biomaterials were used individually. They are unable to satisfactorily mimic the actual substrate for tissue regeneration both structurally and functionally. The formation of hybrid scaffolds consisting natural and synthetic biomaterials has been proposed to improve the outcomes. therefore, this study aimed to form and evaluate three-dimensional (3D) 65:35 poly(lactic-co-glycolic acid) PLGA (“P”) scaffold incorporated with and without atelocollagen (“A”) and/or fibrin (“F”). The PLGA scaffold was formed using solvent casting and salt leaching method, freeze-dried, and crosslinked with the natural biomaterials. The hybrid scaffolds groups were “PAF”, “PA” and “PF”. The PLGA only scaffold was used as control. All scaffolds were evaluated using selected analytical techniques namely Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy analysis, scanning electron microscopy (SEM), porosity study, water uptake capacity, and cytocompatibility analysis in “chondrocyte-scaffold” constructs groups at week 1, 2 and 3 in vitro. The interaction of PLGA scaffold with fibrin and/or atelocollagen was evidenced based on the notable peaks of amide bonds. The microscopic pore structures appeared interconnected. Total porosity in all scaffolds were varied. All groups demonstrated porosity range from 56% to 61%. Proper swelling ratio of the scaffold is crucial to mimic the water uptake capacity in the living tissues. Despite its hydrophobicity, the PLGA only group demonstrated the highest swelling ratio when compared to other groups though, the difference is not significant. The cytocompatibility of all scaffolds except PA showed similar trends of cell proliferation. In this study, the incorporation of fibrin facilitates better microenvironment for cellular growth in terms of cell adherence to the scaffolds compared to the groups without fibrin. Based on the preliminary results, the 65:35 PLGA based hybrid scaffolds may have the potential for cartilage tissue engineering application.
format	Conference or Workshop Item
author	Mohamed Amin, Muhammad Azri Ifwat Md Ali @ Tahir, Aisyah Hanani Azhim, Azran Sha'ban, Munirah
author_facet	Mohamed Amin, Muhammad Azri Ifwat Md Ali @ Tahir, Aisyah Hanani Azhim, Azran Sha'ban, Munirah
author_sort	Mohamed Amin, Muhammad Azri Ifwat
title	Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
title_short	Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
title_full	Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
title_fullStr	Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
title_full_unstemmed	Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
title_sort	physical properties and biocompatibility of 3d hybrid plga based scaffolds
publisher	Institute of Electrical and Electronics Engineers Inc.
publishDate	2019
url	http://irep.iium.edu.my/70448/ http://irep.iium.edu.my/70448/ http://irep.iium.edu.my/70448/ http://irep.iium.edu.my/70448/1/70448_Physical%20properties%20and%20biocompatibility.pdf http://irep.iium.edu.my/70448/2/70448_Physical%20properties%20and%20biocompatibility_SCOPUS.pdf http://irep.iium.edu.my/70448/3/70448_Physical%20properties%20and%20biocompatibility_WOS.pdf
first_indexed	2023-09-18T21:40:01Z
last_indexed	2023-09-18T21:40:01Z
_version_	1777413076959625216

Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds

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