Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct

This study aims to evaluate the cartilaginous extracellular matrix (ECM) production in in vitro poly(lacticco-glycolic acid) PLGA-based scaffolds seeded with rabbit chondrocytes. Scaffolds with the size of 7mm (diameter) × 3mm (height) using ratio of 65:35 were formed using solvent-casting and salt...

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Main Authors:	Md Ali @ Tahir, Aisyah Hanani, Mohamed Amin, Muhammad Azri Ifwat, Azhim, Azran, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English English English
Published:	Institute of Electrical and Electronics Engineers Inc. 2018
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/1/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix.pdf http://irep.iium.edu.my/70459/2/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_SCOPUS.pdf http://irep.iium.edu.my/70459/3/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_WOS.pdf

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recordtype	eprints
spelling	iium-704592019-06-13T08:24:25Z http://irep.iium.edu.my/70459/ Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah TA164 Bioengineering This study aims to evaluate the cartilaginous extracellular matrix (ECM) production in in vitro poly(lacticco-glycolic acid) PLGA-based scaffolds seeded with rabbit chondrocytes. Scaffolds with the size of 7mm (diameter) × 3mm (height) using ratio of 65:35 were formed using solvent-casting and salt leaching method. The scaffolds were divided into four respective groups, 1) PLGA-atelocollagen-fibrin (PAF), 2) PLGA-atelocollagen (PA), 3) PLGA-fibrin (PF), and 4) PLGA (control). Gross morphology of the scaffold was observed for 3 weeks. Microscopy evaluation and histological staining was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue for week 1, 2 and 3, respectively. The production of sulphated glycosaminoglycan (sGAG) was evaluated using Alcian Blue assay. The PAF group presented better cartilaginous appearance compared to other groups. The hybrid scaffold groups showed more ECM secretion indicated by the presence of web-like fibers network substance on the surface as visualized using scanning electron microscope (SEM). The presence of proteoglycan-rich matrix and glycosaminoglycan has been detected in both histological staining and sGAG assay. Based on the findings of this study, PLGA-based hybrid scaffold promoted better cartilaginous tissue formation in vitro. Institute of Electrical and Electronics Engineers Inc. 2018-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/70459/1/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix.pdf application/pdf en http://irep.iium.edu.my/70459/2/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/70459/3/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_WOS.pdf Md Ali @ Tahir, Aisyah Hanani and Mohamed Amin, Muhammad Azri Ifwat and Azhim, Azran and Sha'ban, Munirah (2018) Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct. In: 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences )IECBES 2018), 3rd-6th December 2018, Kuching, Sarawak. https://ieeexplore.ieee.org/document/8626712/authors#authors 10.1109/IECBES.2018.8626712
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
description	This study aims to evaluate the cartilaginous extracellular matrix (ECM) production in in vitro poly(lacticco-glycolic acid) PLGA-based scaffolds seeded with rabbit chondrocytes. Scaffolds with the size of 7mm (diameter) × 3mm (height) using ratio of 65:35 were formed using solvent-casting and salt leaching method. The scaffolds were divided into four respective groups, 1) PLGA-atelocollagen-fibrin (PAF), 2) PLGA-atelocollagen (PA), 3) PLGA-fibrin (PF), and 4) PLGA (control). Gross morphology of the scaffold was observed for 3 weeks. Microscopy evaluation and histological staining was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue for week 1, 2 and 3, respectively. The production of sulphated glycosaminoglycan (sGAG) was evaluated using Alcian Blue assay. The PAF group presented better cartilaginous appearance compared to other groups. The hybrid scaffold groups showed more ECM secretion indicated by the presence of web-like fibers network substance on the surface as visualized using scanning electron microscope (SEM). The presence of proteoglycan-rich matrix and glycosaminoglycan has been detected in both histological staining and sGAG assay. Based on the findings of this study, PLGA-based hybrid scaffold promoted better cartilaginous tissue formation in vitro.
format	Conference or Workshop Item
author	Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah
author_facet	Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah
author_sort	Md Ali @ Tahir, Aisyah Hanani
title	Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
title_short	Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
title_full	Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
title_fullStr	Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
title_full_unstemmed	Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
title_sort	evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
publisher	Institute of Electrical and Electronics Engineers Inc.
publishDate	2018
url	http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/1/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix.pdf http://irep.iium.edu.my/70459/2/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_SCOPUS.pdf http://irep.iium.edu.my/70459/3/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_WOS.pdf
first_indexed	2023-09-18T21:40:01Z
last_indexed	2023-09-18T21:40:01Z
_version_	1777413077691531264

Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct

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