Culturing and maintaining mammalian cell culture

Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and interc...

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Bibliographic Details
Main Authors:	Ahmad Raus, Raha, Ali Salih, Ghassan, Shaban, Mustaffa Nameer
Format:	Book Chapter
Language:	English English
Published:	Springer Nature 2018
Subjects:	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70574/ http://irep.iium.edu.my/70574/ http://irep.iium.edu.my/70574/1/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture.pdf http://irep.iium.edu.my/70574/7/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture_Scopus.pdf

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recordtype	eprints
spelling	iium-705742020-03-05T07:51:53Z http://irep.iium.edu.my/70574/ Culturing and maintaining mammalian cell culture Ahmad Raus, Raha Ali Salih, Ghassan Shaban, Mustaffa Nameer TP200 Manufacture and use of chemicals TP248.13 Biotechnology Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and intercellular activities and flux; genetic and phenotyping analysis; proteomics, study of toxicology, drug discovery and development can be carried out without manipulation of living animals. In this chapter, detail protocol of media preparation, cell culture maintenance and preservation are elaborated for both types of mammalian cell culture, monolayer or suspension cultures. Determination of number of cells is discussed as well. Springer Nature 2018 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/70574/1/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture.pdf application/pdf en http://irep.iium.edu.my/70574/7/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture_Scopus.pdf Ahmad Raus, Raha and Ali Salih, Ghassan and Shaban, Mustaffa Nameer (2018) Culturing and maintaining mammalian cell culture. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 215-226. ISBN 978-981-13-2256-3 https://www.springer.com/us/book/9789811322563#aboutAuthors
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
spellingShingle	TP200 Manufacture and use of chemicals TP248.13 Biotechnology Ahmad Raus, Raha Ali Salih, Ghassan Shaban, Mustaffa Nameer Culturing and maintaining mammalian cell culture
description	Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and intercellular activities and flux; genetic and phenotyping analysis; proteomics, study of toxicology, drug discovery and development can be carried out without manipulation of living animals. In this chapter, detail protocol of media preparation, cell culture maintenance and preservation are elaborated for both types of mammalian cell culture, monolayer or suspension cultures. Determination of number of cells is discussed as well.
format	Book Chapter
author	Ahmad Raus, Raha Ali Salih, Ghassan Shaban, Mustaffa Nameer
author_facet	Ahmad Raus, Raha Ali Salih, Ghassan Shaban, Mustaffa Nameer
author_sort	Ahmad Raus, Raha
title	Culturing and maintaining mammalian cell culture
title_short	Culturing and maintaining mammalian cell culture
title_full	Culturing and maintaining mammalian cell culture
title_fullStr	Culturing and maintaining mammalian cell culture
title_full_unstemmed	Culturing and maintaining mammalian cell culture
title_sort	culturing and maintaining mammalian cell culture
publisher	Springer Nature
publishDate	2018
url	http://irep.iium.edu.my/70574/ http://irep.iium.edu.my/70574/ http://irep.iium.edu.my/70574/1/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture.pdf http://irep.iium.edu.my/70574/7/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture_Scopus.pdf
first_indexed	2023-09-18T21:40:10Z
last_indexed	2023-09-18T21:40:10Z
_version_	1777413086449238016

Culturing and maintaining mammalian cell culture

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