Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes

Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the s...

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Main Authors:	Kamarudin, Kamarul Rahim, Mohamed Rehan, Maryam, Mohamed Rehan, Aisyah
Format:	Article
Language:	English
Published:	Faculty of International Maritime Studies, Kasetsart University 2019
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/70637/ http://irep.iium.edu.my/70637/ http://irep.iium.edu.my/70637/ http://irep.iium.edu.my/70637/1/Maritime%20Technology%20and%20Research%202019.pdf

id	iium-70637
recordtype	eprints
spelling	iium-706372019-07-12T08:03:17Z http://irep.iium.edu.my/70637/ Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah Q Science (General) Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the species identities of processed sea cucumbers from selected Malaysian markets using concatenated gene sequences of non-protein-coding 12S and 16S mitochondrial rRNA genes. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 47 ingroup sequences representing 37 processed sea cucumber specimens, 6 reference samples, and 4 additional specimens suggested the presence of 3 main clusters i.e. gamat family consisting of genus Stichopus and genus Thelenota; and timun laut family comprising family Holothuriidae. A number of 3 gamat species i.e. Stichopus horrens, Stichopus vastus, and Thelenota anax were recorded. Meanwhile, the specimens of Holothuria (Halodeima) atra, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) lessoni, Holothuria (Mertensiothuria) leucospilota, and Holothuria (Metriatyla) scabra were the 5 timun laut species that grouped under the family Holothuriidae. The outcomes of this study can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets. Faculty of International Maritime Studies, Kasetsart University 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70637/1/Maritime%20Technology%20and%20Research%202019.pdf Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohamed Rehan, Aisyah (2019) Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes. Maritime Technology and Research, 1 (2). pp. 1-19. E-ISSN 2651-205X https://tci-thaijo.org/index.php/MTR/article/view/146259/121339 10.33175/mtr.2019.146259
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
description	Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the species identities of processed sea cucumbers from selected Malaysian markets using concatenated gene sequences of non-protein-coding 12S and 16S mitochondrial rRNA genes. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 47 ingroup sequences representing 37 processed sea cucumber specimens, 6 reference samples, and 4 additional specimens suggested the presence of 3 main clusters i.e. gamat family consisting of genus Stichopus and genus Thelenota; and timun laut family comprising family Holothuriidae. A number of 3 gamat species i.e. Stichopus horrens, Stichopus vastus, and Thelenota anax were recorded. Meanwhile, the specimens of Holothuria (Halodeima) atra, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) lessoni, Holothuria (Mertensiothuria) leucospilota, and Holothuria (Metriatyla) scabra were the 5 timun laut species that grouped under the family Holothuriidae. The outcomes of this study can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets.
format	Article
author	Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah
author_facet	Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah
author_sort	Kamarudin, Kamarul Rahim
title	Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_short	Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_full	Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_fullStr	Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_full_unstemmed	Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_sort	species identification of processed sea cucumbers from malaysian market based on concatenated gene sequences of mitochondrial rrna genes
publisher	Faculty of International Maritime Studies, Kasetsart University
publishDate	2019
url	http://irep.iium.edu.my/70637/ http://irep.iium.edu.my/70637/ http://irep.iium.edu.my/70637/ http://irep.iium.edu.my/70637/1/Maritime%20Technology%20and%20Research%202019.pdf
first_indexed	2023-09-18T21:40:17Z
last_indexed	2023-09-18T21:40:17Z
_version_	1777413093649809408

Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes

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