Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats

Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats

Syzygium polyanthum leaf has been used traditionally for treating hypertension. An aqueous extract of S. polyanthum leaf (ASP) has an antihypertensive effect; however, there is no further evaluation on its fractions yet. This study aims to identify the most promising fraction derived from ASP in ter...

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Main Authors: Abd Rahim, Erlena Nor Asmira, Ismail, Azlini, Omar, Muhammad Nor, Wan Ahmad, Wan Amir Nizam
Format: Article
Language:English
Published: School of Health Sciences, Universiti Sains Malaysia 2018
Subjects:
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/71044/
http://irep.iium.edu.my/71044/
http://irep.iium.edu.my/71044/1/71044_Antihypertensive%20effect%20of%20fractions.pdf
id iium-71044
recordtype eprints
spelling iium-710442019-03-11T04:39:29Z http://irep.iium.edu.my/71044/ Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats Abd Rahim, Erlena Nor Asmira Ismail, Azlini Omar, Muhammad Nor Wan Ahmad, Wan Amir Nizam RM Therapeutics. Pharmacology RS Pharmacy and materia medica Syzygium polyanthum leaf has been used traditionally for treating hypertension. An aqueous extract of S. polyanthum leaf (ASP) has an antihypertensive effect; however, there is no further evaluation on its fractions yet. This study aims to identify the most promising fraction derived from ASP in terms of antihypertensive effect. Dried leaves of S. polyanthum were extracted using an ultrasound-assisted extraction method to produce ASP and then it was fractionated using column chromatography to yield F1ASP, F2ASP and F3ASP. Spontaneously hypertensive rats (SHR) was anesthetized with sodium pentobarbital (50 mg/kg, i.p.), followed by left jugular vein cannulation for extract administration. Right carotid artery was then cannulated and connected to BIOPAC Blood Pressure System Version 3.6.7 for recordings of mean arterial pressure (MAP), systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR). One-way and two-way ANOVA with Sidak post-hoc tests were performed using GraphPad Prism Version 6.0. F1ASP (30 to 60 mg/kg), F2ASP (20 to 60 mg/kg) and F3ASP (30 to 40 mg/kg) showed significant antihypertensive effect in comparison to negative control (normal saline). F2ASP caused more reduction (p < 0.05) in MAP of SHR as compared to ASP at doses of 20 and 30 mg/kg. The antihypertensive effect by F2ASP at doses of 20 to 60 mg/kg was comparable to positive control drug, captopril at 5 mg/kg. No significant effect observed on HR. F2ASP has the most promising antihypertensive effect in comparison to F1ASP and F3ASP. School of Health Sciences, Universiti Sains Malaysia 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71044/1/71044_Antihypertensive%20effect%20of%20fractions.pdf Abd Rahim, Erlena Nor Asmira and Ismail, Azlini and Omar, Muhammad Nor and Wan Ahmad, Wan Amir Nizam (2018) Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats. Health and the Environment Journal, 9 (Supplement 1). p. 14. ISSN 2180-1126 http://hej.kk.usm.my/pdf/HEJVol.9Supp.1/5%20Abstract%20for%20Session%20B.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Abd Rahim, Erlena Nor Asmira
Ismail, Azlini
Omar, Muhammad Nor
Wan Ahmad, Wan Amir Nizam
Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats
description Syzygium polyanthum leaf has been used traditionally for treating hypertension. An aqueous extract of S. polyanthum leaf (ASP) has an antihypertensive effect; however, there is no further evaluation on its fractions yet. This study aims to identify the most promising fraction derived from ASP in terms of antihypertensive effect. Dried leaves of S. polyanthum were extracted using an ultrasound-assisted extraction method to produce ASP and then it was fractionated using column chromatography to yield F1ASP, F2ASP and F3ASP. Spontaneously hypertensive rats (SHR) was anesthetized with sodium pentobarbital (50 mg/kg, i.p.), followed by left jugular vein cannulation for extract administration. Right carotid artery was then cannulated and connected to BIOPAC Blood Pressure System Version 3.6.7 for recordings of mean arterial pressure (MAP), systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR). One-way and two-way ANOVA with Sidak post-hoc tests were performed using GraphPad Prism Version 6.0. F1ASP (30 to 60 mg/kg), F2ASP (20 to 60 mg/kg) and F3ASP (30 to 40 mg/kg) showed significant antihypertensive effect in comparison to negative control (normal saline). F2ASP caused more reduction (p < 0.05) in MAP of SHR as compared to ASP at doses of 20 and 30 mg/kg. The antihypertensive effect by F2ASP at doses of 20 to 60 mg/kg was comparable to positive control drug, captopril at 5 mg/kg. No significant effect observed on HR. F2ASP has the most promising antihypertensive effect in comparison to F1ASP and F3ASP.
format Article
author Abd Rahim, Erlena Nor Asmira
Ismail, Azlini
Omar, Muhammad Nor
Wan Ahmad, Wan Amir Nizam
author_facet Abd Rahim, Erlena Nor Asmira
Ismail, Azlini
Omar, Muhammad Nor
Wan Ahmad, Wan Amir Nizam
author_sort Abd Rahim, Erlena Nor Asmira
title Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats
title_short Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats
title_full Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats
title_fullStr Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats
title_full_unstemmed Antihypertensive effect of fractions derived from an aqueous extract of Syzygium polyanthum on anaesthetized spontaneously hypertensive rats
title_sort antihypertensive effect of fractions derived from an aqueous extract of syzygium polyanthum on anaesthetized spontaneously hypertensive rats
publisher School of Health Sciences, Universiti Sains Malaysia
publishDate 2018
url http://irep.iium.edu.my/71044/
http://irep.iium.edu.my/71044/
http://irep.iium.edu.my/71044/1/71044_Antihypertensive%20effect%20of%20fractions.pdf
first_indexed 2023-09-18T21:40:50Z
last_indexed 2023-09-18T21:40:50Z
_version_ 1777413128650227712

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