Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile

Fucoxanthin has interesting anticancer activity, but is insoluble in water, hindering its use as a drug. Microencapsulation is used as a technique for improving drug delivery. This study aimed to formulate fucoxanthin-loaded microspheres (F-LM) for anticancer treatment of H1299 cancer cell lines and...

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Main Authors:	Jaswir, Irwandi, ,, Dedi Noviendri, Bakhtiar, Muhammad Taher, Mohamed, Farahidah, Octavianti, Fitri, Lestari, Widya, Mukti, Ali Ghufron, Nirwandar, Sapta, Almansori, Bubaker B. Hamad
Format:	Article
Language:	English English English
Published:	MDPI 2019
Subjects:	RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/7/71146%20Optimization%20and%20Formulation.pdf http://irep.iium.edu.my/71146/8/71146%20Optimization%20and%20Formulation%20SCOPUS.pdf http://irep.iium.edu.my/71146/19/71146_Optimization%20and%20Formulation%20of%20Fucoxanthin-Loaded%20Microsphere%20%28F-LM%29%20Using%20Response%20Surface%20Methodology%20%28RSM%29%20and%20Analysis%20of%20Its%20Fucoxanthin%20Release%20Profile_wos.pdf

id	iium-71146
recordtype	eprints
spelling	iium-711462019-08-01T03:41:33Z http://irep.iium.edu.my/71146/ Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile Jaswir, Irwandi ,, Dedi Noviendri Bakhtiar, Muhammad Taher Mohamed, Farahidah Octavianti, Fitri Lestari, Widya Mukti, Ali Ghufron Nirwandar, Sapta Almansori, Bubaker B. Hamad RS403 Materia Medica-Pharmaceutical Chemistry Fucoxanthin has interesting anticancer activity, but is insoluble in water, hindering its use as a drug. Microencapsulation is used as a technique for improving drug delivery. This study aimed to formulate fucoxanthin-loaded microspheres (F-LM) for anticancer treatment of H1299 cancer cell lines and optimize particle size (PS) and encapsulation efficiency (EE). Using response surface methodology (RSM), a face centered central composite design (FCCCD) was designed with three factors: Polyvinylalcohol (PVA), poly(D,L-lactic-co-glycolic acid) (PLGA), and fucoxanthin concentration. F-LM was produced using a modified double-emulsion solvent evaporation method. The F-LM were characterized for release profile, release kinetics, and degradation pattern. Optimal F-LM PS and EE of 9.18 µm and 33.09%, respectively, with good surface morphology, were achieved from a 0.5% (w/v) PVA, 6.0% (w/v) PLGA, 200 µg/mL fucoxanthin formulation at a homogenization speed of 20,500 rpm. PVA concentration was the most significant factor (p < 0.05) affecting PS. Meanwhile, EE was significantly affected by interaction between the three factors: PVA, PLGA, and fucoxanthin. In vitro release curve showed fucoxanthin had a high burst release (38.3%) at the first hour, followed by a sustained release stage reaching (79.1%) within 2 months. Release kinetics followed a diffusion pattern predominantly controlled by the Higuchi model. Biodegradability studies based on surface morphology changes on the surface of the F-LM, show that morphology changed within the first hour, and F-LM completely degraded within 2 months. RSM under FCCCD design improved the difference between the lowest and highest responses, with good correlation between observed and predicted values for PS and EE of F-LM. MDPI 2019-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71146/7/71146%20Optimization%20and%20Formulation.pdf application/pdf en http://irep.iium.edu.my/71146/8/71146%20Optimization%20and%20Formulation%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/71146/19/71146_Optimization%20and%20Formulation%20of%20Fucoxanthin-Loaded%20Microsphere%20%28F-LM%29%20Using%20Response%20Surface%20Methodology%20%28RSM%29%20and%20Analysis%20of%20Its%20Fucoxanthin%20Release%20Profile_wos.pdf Jaswir, Irwandi and ,, Dedi Noviendri and Bakhtiar, Muhammad Taher and Mohamed, Farahidah and Octavianti, Fitri and Lestari, Widya and Mukti, Ali Ghufron and Nirwandar, Sapta and Almansori, Bubaker B. Hamad (2019) Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile. Molecules, 24 (5). pp. 1-16. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/5/947 10.3390/molecules24050947
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	RS403 Materia Medica-Pharmaceutical Chemistry Jaswir, Irwandi ,, Dedi Noviendri Bakhtiar, Muhammad Taher Mohamed, Farahidah Octavianti, Fitri Lestari, Widya Mukti, Ali Ghufron Nirwandar, Sapta Almansori, Bubaker B. Hamad Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
description	Fucoxanthin has interesting anticancer activity, but is insoluble in water, hindering its use as a drug. Microencapsulation is used as a technique for improving drug delivery. This study aimed to formulate fucoxanthin-loaded microspheres (F-LM) for anticancer treatment of H1299 cancer cell lines and optimize particle size (PS) and encapsulation efficiency (EE). Using response surface methodology (RSM), a face centered central composite design (FCCCD) was designed with three factors: Polyvinylalcohol (PVA), poly(D,L-lactic-co-glycolic acid) (PLGA), and fucoxanthin concentration. F-LM was produced using a modified double-emulsion solvent evaporation method. The F-LM were characterized for release profile, release kinetics, and degradation pattern. Optimal F-LM PS and EE of 9.18 µm and 33.09%, respectively, with good surface morphology, were achieved from a 0.5% (w/v) PVA, 6.0% (w/v) PLGA, 200 µg/mL fucoxanthin formulation at a homogenization speed of 20,500 rpm. PVA concentration was the most significant factor (p < 0.05) affecting PS. Meanwhile, EE was significantly affected by interaction between the three factors: PVA, PLGA, and fucoxanthin. In vitro release curve showed fucoxanthin had a high burst release (38.3%) at the first hour, followed by a sustained release stage reaching (79.1%) within 2 months. Release kinetics followed a diffusion pattern predominantly controlled by the Higuchi model. Biodegradability studies based on surface morphology changes on the surface of the F-LM, show that morphology changed within the first hour, and F-LM completely degraded within 2 months. RSM under FCCCD design improved the difference between the lowest and highest responses, with good correlation between observed and predicted values for PS and EE of F-LM.
format	Article
author	Jaswir, Irwandi ,, Dedi Noviendri Bakhtiar, Muhammad Taher Mohamed, Farahidah Octavianti, Fitri Lestari, Widya Mukti, Ali Ghufron Nirwandar, Sapta Almansori, Bubaker B. Hamad
author_facet	Jaswir, Irwandi ,, Dedi Noviendri Bakhtiar, Muhammad Taher Mohamed, Farahidah Octavianti, Fitri Lestari, Widya Mukti, Ali Ghufron Nirwandar, Sapta Almansori, Bubaker B. Hamad
author_sort	Jaswir, Irwandi
title	Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
title_short	Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
title_full	Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
title_fullStr	Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
title_full_unstemmed	Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile
title_sort	optimization and formulation of fucoxanthin-loaded microsphere (f-lm) using response surface methodology (rsm) and analysis of its fucoxanthin release profile
publisher	MDPI
publishDate	2019
url	http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/ http://irep.iium.edu.my/71146/7/71146%20Optimization%20and%20Formulation.pdf http://irep.iium.edu.my/71146/8/71146%20Optimization%20and%20Formulation%20SCOPUS.pdf http://irep.iium.edu.my/71146/19/71146_Optimization%20and%20Formulation%20of%20Fucoxanthin-Loaded%20Microsphere%20%28F-LM%29%20Using%20Response%20Surface%20Methodology%20%28RSM%29%20and%20Analysis%20of%20Its%20Fucoxanthin%20Release%20Profile_wos.pdf
first_indexed	2023-09-18T21:40:59Z
last_indexed	2023-09-18T21:40:59Z
_version_	1777413138577096704

Optimization and formulation of Fucoxanthin-Loaded Microsphere (F-LM) using Response Surface Methodology (RSM) and analysis of its fucoxanthin release profile

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