Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana

Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana

Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N-methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which...

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Main Authors: Ahmad, Lyena Watty Zuraine, Zainuddin, Zarina, Osman, Mohamad, Mohamad, Azhar, Jaafar Sidek, Norrizzah
Format: Article
Language:English
English
English
Published: Penerbit UKM Press 2019
Subjects:
S Agriculture (General)
Online Access:http://irep.iium.edu.my/71186/
http://irep.iium.edu.my/71186/
http://irep.iium.edu.my/71186/
http://irep.iium.edu.my/71186/1/71186_Effects%20of%20N-methyl-N-nitrosourea.pdf
http://irep.iium.edu.my/71186/7/71186_Effects%20of%20N-methyl-N-nitrosourea%20on%20seed_wos.pdf
http://irep.iium.edu.my/71186/8/71186_Effects%20of%20N-methyl-N-nitrosourea%20on%20seed_scopus.pdf
id iium-71186
recordtype eprints
spelling iium-711862019-04-25T05:46:53Z http://irep.iium.edu.my/71186/ Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana Ahmad, Lyena Watty Zuraine Zainuddin, Zarina Osman, Mohamad Mohamad, Azhar Jaafar Sidek, Norrizzah S Agriculture (General) Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N-methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and ef cient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU. ******************************************************** Stevia rebaudiana ialah tumbuhan unik yang mengandungi pemanis semula jadi tanpa kalori yang menarik minat ramai rakyat Malaysia. Dalam kajian ini, kesan kepekatan N-metil-N-nitrosourea (MNU) dikaji dalam mendorong mutasi pada biji Stevia untuk menghasilkan variasi genetik yang berguna untuk pembaikan tanaman. Biji Stevia direndam dalam larutan MNU dengan enam kepekataan berlainan (0.0, 0.13, 0.25, 0.38, 0.50 dan 1.00 mM) untuk empat tempoh yang berbeza (15, 30, 45 dan 60 minit) pada suhu bilik. Penggunaan MNU didapati mengurangkan peratus dan kadar percambahan biji Stevia berbanding dengan kumpulan kawalan. Pendedahan berpanjangan kepada kepekatan MNU yang tertinggi mencatatkan peratusan percambahan terendah (2.5 ± 1.4%) dan kadar percambahan terendah (0.21 ± 0.16). Anak benih dengan trikot dicerap pada kumpulan yang dirawat dengan 0.13, 0.38 dan 1.0 mM MNU selama 30 minit. Kehadiran anak benih dengan warna albino membuktikan kesan mutagen MNU pada genom Stevia. Berdasarkan peratusan anak benih dengan mutasi klorofil, rawatan mutagenik yang paling berkesan dan cekap untuk merangsang mutasi ialah selama 60 minit dalam 0.25 mM MNU. Penerbit UKM Press 2019-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71186/1/71186_Effects%20of%20N-methyl-N-nitrosourea.pdf application/pdf en http://irep.iium.edu.my/71186/7/71186_Effects%20of%20N-methyl-N-nitrosourea%20on%20seed_wos.pdf application/pdf en http://irep.iium.edu.my/71186/8/71186_Effects%20of%20N-methyl-N-nitrosourea%20on%20seed_scopus.pdf Ahmad, Lyena Watty Zuraine and Zainuddin, Zarina and Osman, Mohamad and Mohamad, Azhar and Jaafar Sidek, Norrizzah (2019) Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana. Sains Malaysiana, 48 (2). pp. 301-307. ISSN 0126-6039 http://www.ukm.my/jsm/pdf_files/SM-PDF-48-2-2019/05%20Lyena%20Watty%20Zuraine%20Ahmad.pdf http://www.ukm.my/jsm/pdf_files/SM-PDF-48-2-2019/05%20Lyena%20Watty%20Zuraine%20Ahmad.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Ahmad, Lyena Watty Zuraine
Zainuddin, Zarina
Osman, Mohamad
Mohamad, Azhar
Jaafar Sidek, Norrizzah
Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana
description Stevia rebaudiana is a unique plant that contains non-caloric natural sweetener and has gained much interest among Malaysians. In this study, the effect of different concentrations of N-methyl-N-nitrosourea (MNU) was assessed in inducing mutation in Stevia seeds to produce genetic variations, which is valuable for crop improvement. Stevia seeds were soaked in six concentrations of MNU (0.0, 0.13, 0.25, 0.38, 0.50, and 1.00 mM) for four different durations (15, 30, 45, and 60 min) at room temperature. As a result, application of MNU reduced the germination percentage and germination rate of Stevia seeds as compared to the control group. Prolonged exposure to the highest concentration of MNU recorded the lowest percentage of germination (2.5 ± 1.4%) and the lowest germination rate (0.21 ± 0.16). Tricots were observed among seedlings treated with 0.13, 0.38 and 1.0 mM of MNU for 30 min. Presence of seedlings with albino colour proved the mutagenic effect of MNU on Stevia genome. Based on the percentage of seedlings with chlorophyll mutation, the most effective and ef cient mutagenic treatment to induce mutation was 60 min in 0.25 mM of MNU. ******************************************************** Stevia rebaudiana ialah tumbuhan unik yang mengandungi pemanis semula jadi tanpa kalori yang menarik minat ramai rakyat Malaysia. Dalam kajian ini, kesan kepekatan N-metil-N-nitrosourea (MNU) dikaji dalam mendorong mutasi pada biji Stevia untuk menghasilkan variasi genetik yang berguna untuk pembaikan tanaman. Biji Stevia direndam dalam larutan MNU dengan enam kepekataan berlainan (0.0, 0.13, 0.25, 0.38, 0.50 dan 1.00 mM) untuk empat tempoh yang berbeza (15, 30, 45 dan 60 minit) pada suhu bilik. Penggunaan MNU didapati mengurangkan peratus dan kadar percambahan biji Stevia berbanding dengan kumpulan kawalan. Pendedahan berpanjangan kepada kepekatan MNU yang tertinggi mencatatkan peratusan percambahan terendah (2.5 ± 1.4%) dan kadar percambahan terendah (0.21 ± 0.16). Anak benih dengan trikot dicerap pada kumpulan yang dirawat dengan 0.13, 0.38 dan 1.0 mM MNU selama 30 minit. Kehadiran anak benih dengan warna albino membuktikan kesan mutagen MNU pada genom Stevia. Berdasarkan peratusan anak benih dengan mutasi klorofil, rawatan mutagenik yang paling berkesan dan cekap untuk merangsang mutasi ialah selama 60 minit dalam 0.25 mM MNU.
format Article
author Ahmad, Lyena Watty Zuraine
Zainuddin, Zarina
Osman, Mohamad
Mohamad, Azhar
Jaafar Sidek, Norrizzah
author_facet Ahmad, Lyena Watty Zuraine
Zainuddin, Zarina
Osman, Mohamad
Mohamad, Azhar
Jaafar Sidek, Norrizzah
author_sort Ahmad, Lyena Watty Zuraine
title Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana
title_short Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana
title_full Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana
title_fullStr Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana
title_full_unstemmed Effects of N-methyl-N-nitrosourea on seed germitantion of Stevia rebaudiana = Kesan N-Metil-N-Nitrosourea pada percambahan biji benih stevia rebaudiana
title_sort effects of n-methyl-n-nitrosourea on seed germitantion of stevia rebaudiana = kesan n-metil-n-nitrosourea pada percambahan biji benih stevia rebaudiana
publisher Penerbit UKM Press
publishDate 2019
url http://irep.iium.edu.my/71186/
http://irep.iium.edu.my/71186/
http://irep.iium.edu.my/71186/
http://irep.iium.edu.my/71186/1/71186_Effects%20of%20N-methyl-N-nitrosourea.pdf
http://irep.iium.edu.my/71186/7/71186_Effects%20of%20N-methyl-N-nitrosourea%20on%20seed_wos.pdf
http://irep.iium.edu.my/71186/8/71186_Effects%20of%20N-methyl-N-nitrosourea%20on%20seed_scopus.pdf
first_indexed 2023-09-18T21:41:03Z
last_indexed 2023-09-18T21:41:03Z
_version_ 1777413142075146240

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