Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study

Porcupine bezoars (PBs) are masses of undigested calcareous concretions formed within the gastrointestinal tract. There are undocumented claims that PBs have antioxidant activity and can treat cancers. However, limited scientific study has been carried out to verify these traditional claims. Hence,...

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Main Authors:	Firus Khan, Al'aina Yuhanis, Abdullah Asuhaimi, Faizah, Jalal, Tara Kamal, Roheem, Fatimah, Natto, Hatim Abdullah, Johan, Muhammad Farid, Ahmed, Qamar Uddin, Abdul Wahab, Ridhwan
Format:	Article
Language:	English English English
Published:	MDPI AG, Basel Switzerland 2019
Subjects:	Q Science (General) RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/71210/ http://irep.iium.edu.my/71210/ http://irep.iium.edu.my/71210/ http://irep.iium.edu.my/71210/1/71210_Hystrix%20Brachyura%20Bezoar%20Characterization.pdf http://irep.iium.edu.my/71210/2/71210_Hystrix%20Brachyura%20Bezoar%20Characterization_SCOPUS.pdf http://irep.iium.edu.my/71210/3/71210_Hystrix%20Brachyura%20Bezoar%20Characterization_WOS.pdf

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spelling	iium-712102019-07-12T07:11:31Z http://irep.iium.edu.my/71210/ Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study Firus Khan, Al'aina Yuhanis Abdullah Asuhaimi, Faizah Jalal, Tara Kamal Roheem, Fatimah Natto, Hatim Abdullah Johan, Muhammad Farid Ahmed, Qamar Uddin Abdul Wahab, Ridhwan Q Science (General) RM Therapeutics. Pharmacology Porcupine bezoars (PBs) are masses of undigested calcareous concretions formed within the gastrointestinal tract. There are undocumented claims that PBs have antioxidant activity and can treat cancers. However, limited scientific study has been carried out to verify these traditional claims. Hence, this study was conducted to characterize the chemical profile and validate the antioxidant and anticancer activity against melanoma cells (A375). PB extract was initially subjected to Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GCMS), total phenolic content (TPC), and total flavonoid content (TFC) analyses. The bioautography of antioxidant assays, namely 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH), and β-carotene was performed. An in vitro A375 cell viability assay, apoptosis assay, cell cycle arrest assay, and gene expression assay were carried out as well. The experimental finding revealed 5,10-diethoxy-2,3,7,8-tetrahydro-1H,6H-dipyrrolo[1,2-a:1′,2′-d]pyrazine, ursodeoxycholic acid, and cholest-5-en-3-ol (3 beta)-, carbonochloridate are major compounds detected in PB extract. PB extract has low phenolic content, viz. 698.7 ± 0.93 (µg GAE/5 mg dry weight). The bioautography antioxidant assays revealed a potent antioxidant effect (ABTS > DPPH > β-carotene), with free radical scavenging activity. Furthermore, PB extract exhibited dose- and time-dependent inhibition of cancer activity on A375 cells due to the exhibition of apoptosis via an intrinsic pathway. MDPI AG, Basel Switzerland 2019-02-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71210/1/71210_Hystrix%20Brachyura%20Bezoar%20Characterization.pdf application/pdf en http://irep.iium.edu.my/71210/2/71210_Hystrix%20Brachyura%20Bezoar%20Characterization_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/71210/3/71210_Hystrix%20Brachyura%20Bezoar%20Characterization_WOS.pdf Firus Khan, Al'aina Yuhanis and Abdullah Asuhaimi, Faizah and Jalal, Tara Kamal and Roheem, Fatimah and Natto, Hatim Abdullah and Johan, Muhammad Farid and Ahmed, Qamar Uddin and Abdul Wahab, Ridhwan (2019) Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study. Antioxidants, 8 (2). pp. 1-15. E-ISSN 2076-3921 https://www.mdpi.com/2076-3921/8/2/39 https://doi.org/10.3390/antiox8020039
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General) RM Therapeutics. Pharmacology
spellingShingle	Q Science (General) RM Therapeutics. Pharmacology Firus Khan, Al'aina Yuhanis Abdullah Asuhaimi, Faizah Jalal, Tara Kamal Roheem, Fatimah Natto, Hatim Abdullah Johan, Muhammad Farid Ahmed, Qamar Uddin Abdul Wahab, Ridhwan Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study
description	Porcupine bezoars (PBs) are masses of undigested calcareous concretions formed within the gastrointestinal tract. There are undocumented claims that PBs have antioxidant activity and can treat cancers. However, limited scientific study has been carried out to verify these traditional claims. Hence, this study was conducted to characterize the chemical profile and validate the antioxidant and anticancer activity against melanoma cells (A375). PB extract was initially subjected to Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GCMS), total phenolic content (TPC), and total flavonoid content (TFC) analyses. The bioautography of antioxidant assays, namely 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH), and β-carotene was performed. An in vitro A375 cell viability assay, apoptosis assay, cell cycle arrest assay, and gene expression assay were carried out as well. The experimental finding revealed 5,10-diethoxy-2,3,7,8-tetrahydro-1H,6H-dipyrrolo[1,2-a:1′,2′-d]pyrazine, ursodeoxycholic acid, and cholest-5-en-3-ol (3 beta)-, carbonochloridate are major compounds detected in PB extract. PB extract has low phenolic content, viz. 698.7 ± 0.93 (µg GAE/5 mg dry weight). The bioautography antioxidant assays revealed a potent antioxidant effect (ABTS > DPPH > β-carotene), with free radical scavenging activity. Furthermore, PB extract exhibited dose- and time-dependent inhibition of cancer activity on A375 cells due to the exhibition of apoptosis via an intrinsic pathway.
format	Article
author	Firus Khan, Al'aina Yuhanis Abdullah Asuhaimi, Faizah Jalal, Tara Kamal Roheem, Fatimah Natto, Hatim Abdullah Johan, Muhammad Farid Ahmed, Qamar Uddin Abdul Wahab, Ridhwan
author_facet	Firus Khan, Al'aina Yuhanis Abdullah Asuhaimi, Faizah Jalal, Tara Kamal Roheem, Fatimah Natto, Hatim Abdullah Johan, Muhammad Farid Ahmed, Qamar Uddin Abdul Wahab, Ridhwan
author_sort	Firus Khan, Al'aina Yuhanis
title	Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study
title_short	Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study
title_full	Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study
title_fullStr	Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study
title_full_unstemmed	Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study
title_sort	hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (a375): a preliminary study
publisher	MDPI AG, Basel Switzerland
publishDate	2019
url	http://irep.iium.edu.my/71210/ http://irep.iium.edu.my/71210/ http://irep.iium.edu.my/71210/ http://irep.iium.edu.my/71210/1/71210_Hystrix%20Brachyura%20Bezoar%20Characterization.pdf http://irep.iium.edu.my/71210/2/71210_Hystrix%20Brachyura%20Bezoar%20Characterization_SCOPUS.pdf http://irep.iium.edu.my/71210/3/71210_Hystrix%20Brachyura%20Bezoar%20Characterization_WOS.pdf
first_indexed	2023-09-18T21:41:05Z
last_indexed	2023-09-18T21:41:05Z
_version_	1777413144587534336

Hystrix brachyura bezoar characterization, antioxidant activity screening, and anticancer activity on melanoma cells (A375): a preliminary study

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