Optimization of the amino functionalization of magnetic nanoparticles for Use in MNP-CLEA technology
The purpose of this research is to optimize the functionalization process of magnetic iron oxide nanoparticles for CLEA immobilization to enzymes, as a solution to the present challenges of CLEA technology such as clumping. A co-precipitation method was used to synthesize the magnetic nanoparticle...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyyah of Engineering, International Islamic University Malaysia
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/71471/ http://irep.iium.edu.my/71471/ http://irep.iium.edu.my/71471/1/71471_Optimization%20of%20the%20Amino%20Functionalization.pdf |
| Summary: | The purpose of this research is to optimize the functionalization process of magnetic iron oxide nanoparticles
for CLEA immobilization to enzymes, as a solution to the present challenges of CLEA technology such as
clumping. A co-precipitation method was used to synthesize the magnetic nanoparticles (MNP), in which ferric
chloride (FeCl3.6H2O) and ferrous sulfate (FeSO4.7H2O) were used to provide Fe3+ and Fe2+ ions. Ammonium
hydroxide (NH4OH) was used as the precipitator of the MNPs. For the MNPs to be immobilized with proteins
through cross-linking, they had to be functionalized with amino groups by 3-aminopropyltrimetoxysilane
(APTES), through a silanization reaction. Silicon ions in APTES connected with oxygen in the iron oxide
particles, thus forming a connection between the nanoparticles and the amino groups inside APTES. The
functionalization of the MNPs with amino groups was optimized using One Factor at a Time (OFAT), with
APTES as the significant parameter. The percentage of BSA immobilized on the functionalized nanoparticles
was used to determine the ability of the functionalized MNPs to immobilize proteins for each possible optimal
run. The optimum concentration for APTES in the amino functionalization of magnetic nanoparticles was
found to be 2.145 M. |
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