Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken

Bacteriocins produced by a variety of microbes are gaining more attention for not only as alternative foodpreservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated from Mala...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Alhaji Yusuf, Moshood, Arief Ichwan, Solachuddin Jauhari
Format:	Conference or Workshop Item
Language:	English
Published:	Journal of Probiotics & Health 2014
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/6/71575%20%20Anti%20proliferative%20bacteriocin.pdf

id	iium-71575
recordtype	eprints
spelling	iium-715752019-04-12T07:52:57Z http://irep.iium.edu.my/71575/ Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Q Science (General) QR Microbiology Bacteriocins produced by a variety of microbes are gaining more attention for not only as alternative foodpreservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15 min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to bacteriocinL-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line wasthe most sensitive with a cytotoxic index of IC50 of 9.009 µg/ml, followed by breast cancer IC50 (11.51 µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57 µg/ml). In conclusion, putative 10KDaEnt C4L10 is a class II bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, its future use as an antitumoragent should also be explored. Journal of Probiotics & Health 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/71575/6/71575%20%20Anti%20proliferative%20bacteriocin.pdf Tengku Abdul Hamid, Tengku Haziyamin and Alhaji Yusuf, Moshood and Arief Ichwan, Solachuddin Jauhari (2014) Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken. In: 3rd International Conference & Exhibition on Probiotics, Functional & Baby Foods, 23rd-25th September 2014, Naples Italy. https://www.omicsonline.org/proceedings/antiproliferative-bacteriocins-active-against-mrsa-from-coagulase-negative-ent-mundtii-strain-c4l10-isolated-from-nonbroiler-chicken-21288.html 10.4172/2329-8901.S1.015
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
description	Bacteriocins produced by a variety of microbes are gaining more attention for not only as alternative foodpreservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15 min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to bacteriocinL-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line wasthe most sensitive with a cytotoxic index of IC50 of 9.009 µg/ml, followed by breast cancer IC50 (11.51 µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57 µg/ml). In conclusion, putative 10KDaEnt C4L10 is a class II bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, its future use as an antitumoragent should also be explored.
format	Conference or Workshop Item
author	Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
title_short	Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
title_full	Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
title_fullStr	Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
title_full_unstemmed	Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
title_sort	anti-proliferative bacteriocin active against mrsa from coagulase negative ent. mundtdii strain c4l10 isolated from non broiler chicken
publisher	Journal of Probiotics & Health
publishDate	2014
url	http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/6/71575%20%20Anti%20proliferative%20bacteriocin.pdf
first_indexed	2023-09-18T21:41:28Z
last_indexed	2023-09-18T21:41:28Z
_version_	1777413168959586304

Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken

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