In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion

Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis e...

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Main Authors:	Mohd Shafri, Mohd Affendi, Mohamed, Farahidah, Hamidon, Nurhani Emira, Adina, Anugerah Budipratama
Format:	Conference or Workshop Item
Language:	English English
Published:	2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/71912/ http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf

id	iium-71912
recordtype	eprints
spelling	iium-719122019-05-02T08:43:41Z http://irep.iium.edu.my/71912/ In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama RS Pharmacy and materia medica Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis emulsion. Different amount of lecithin and hydroxypropylmethylcellulose (HPMC), both acting as surfactants, were used in the formulations and tested for drug release profile. The in-vitro drug release profile was conducted using USP Apparatus 1 with PBS of pH 6.8 under sink condition. A predetermined time point h0was set up to take out the solution from the vessel for quantification. In order to quantify the in-vitro performance, a method validation was completed using UV-spectrophotometry following the requirements listed under ICH Q2 (R1) guidelines. A standard calibration curve with a good linearity, R2= 0.9973 was established. LOD and LOQ were in the range of 0.009 and 0.027 µg/mL respectively, whereas the slope of the standard curve was 0.051. The recovery rate for intra-day was from 98.27 to 101.39%, and from 100.85 to 102.57% for inter-day. The RSD was 1.6. It was found that lecithin-added formulation showsed complete drug release after 5 hours. In contrast, lecithin+HPMC-added formulation gave a total release after 2 hours. Thus, the lecithin-added formulation showed a slower release profile compared to the lecithin+HPMC-added formulation and may be the formulation of choice for future testings. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf application/pdf en http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf Mohd Shafri, Mohd Affendi and Mohamed, Farahidah and Hamidon, Nurhani Emira and Adina, Anugerah Budipratama (2017) In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion. In: 2nd International Anatomical and Biomedical Scientific Conference 2017 (IABS), 1st-2nd August 2017, Serdang, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
description	Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis emulsion. Different amount of lecithin and hydroxypropylmethylcellulose (HPMC), both acting as surfactants, were used in the formulations and tested for drug release profile. The in-vitro drug release profile was conducted using USP Apparatus 1 with PBS of pH 6.8 under sink condition. A predetermined time point h0was set up to take out the solution from the vessel for quantification. In order to quantify the in-vitro performance, a method validation was completed using UV-spectrophotometry following the requirements listed under ICH Q2 (R1) guidelines. A standard calibration curve with a good linearity, R2= 0.9973 was established. LOD and LOQ were in the range of 0.009 and 0.027 µg/mL respectively, whereas the slope of the standard curve was 0.051. The recovery rate for intra-day was from 98.27 to 101.39%, and from 100.85 to 102.57% for inter-day. The RSD was 1.6. It was found that lecithin-added formulation showsed complete drug release after 5 hours. In contrast, lecithin+HPMC-added formulation gave a total release after 2 hours. Thus, the lecithin-added formulation showed a slower release profile compared to the lecithin+HPMC-added formulation and may be the formulation of choice for future testings.
format	Conference or Workshop Item
author	Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama
author_facet	Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama
author_sort	Mohd Shafri, Mohd Affendi
title	In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_short	In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_full	In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_fullStr	In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_full_unstemmed	In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_sort	in-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
publishDate	2017
url	http://irep.iium.edu.my/71912/ http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf
first_indexed	2023-09-18T21:41:58Z
last_indexed	2023-09-18T21:41:58Z
_version_	1777413200294182912

In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion

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