In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion

Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis e...

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Main Authors: Mohd Shafri, Mohd Affendi, Mohamed, Farahidah, Hamidon, Nurhani Emira, Adina, Anugerah Budipratama
Format: Conference or Workshop Item
Language:English
English
Published: 2017
Subjects:
Online Access:http://irep.iium.edu.my/71912/
http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf
http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf
id iium-71912
recordtype eprints
spelling iium-719122019-05-02T08:43:41Z http://irep.iium.edu.my/71912/ In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama RS Pharmacy and materia medica Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis emulsion. Different amount of lecithin and hydroxypropylmethylcellulose (HPMC), both acting as surfactants, were used in the formulations and tested for drug release profile. The in-vitro drug release profile was conducted using USP Apparatus 1 with PBS of pH 6.8 under sink condition. A predetermined time point h0was set up to take out the solution from the vessel for quantification. In order to quantify the in-vitro performance, a method validation was completed using UV-spectrophotometry following the requirements listed under ICH Q2 (R1) guidelines. A standard calibration curve with a good linearity, R2= 0.9973 was established. LOD and LOQ were in the range of 0.009 and 0.027 µg/mL respectively, whereas the slope of the standard curve was 0.051. The recovery rate for intra-day was from 98.27 to 101.39%, and from 100.85 to 102.57% for inter-day. The RSD was 1.6. It was found that lecithin-added formulation showsed complete drug release after 5 hours. In contrast, lecithin+HPMC-added formulation gave a total release after 2 hours. Thus, the lecithin-added formulation showed a slower release profile compared to the lecithin+HPMC-added formulation and may be the formulation of choice for future testings. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf application/pdf en http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf Mohd Shafri, Mohd Affendi and Mohamed, Farahidah and Hamidon, Nurhani Emira and Adina, Anugerah Budipratama (2017) In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion. In: 2nd International Anatomical and Biomedical Scientific Conference 2017 (IABS), 1st-2nd August 2017, Serdang, Selangor. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Mohd Shafri, Mohd Affendi
Mohamed, Farahidah
Hamidon, Nurhani Emira
Adina, Anugerah Budipratama
In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
description Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis emulsion. Different amount of lecithin and hydroxypropylmethylcellulose (HPMC), both acting as surfactants, were used in the formulations and tested for drug release profile. The in-vitro drug release profile was conducted using USP Apparatus 1 with PBS of pH 6.8 under sink condition. A predetermined time point h0was set up to take out the solution from the vessel for quantification. In order to quantify the in-vitro performance, a method validation was completed using UV-spectrophotometry following the requirements listed under ICH Q2 (R1) guidelines. A standard calibration curve with a good linearity, R2= 0.9973 was established. LOD and LOQ were in the range of 0.009 and 0.027 µg/mL respectively, whereas the slope of the standard curve was 0.051. The recovery rate for intra-day was from 98.27 to 101.39%, and from 100.85 to 102.57% for inter-day. The RSD was 1.6. It was found that lecithin-added formulation showsed complete drug release after 5 hours. In contrast, lecithin+HPMC-added formulation gave a total release after 2 hours. Thus, the lecithin-added formulation showed a slower release profile compared to the lecithin+HPMC-added formulation and may be the formulation of choice for future testings.
format Conference or Workshop Item
author Mohd Shafri, Mohd Affendi
Mohamed, Farahidah
Hamidon, Nurhani Emira
Adina, Anugerah Budipratama
author_facet Mohd Shafri, Mohd Affendi
Mohamed, Farahidah
Hamidon, Nurhani Emira
Adina, Anugerah Budipratama
author_sort Mohd Shafri, Mohd Affendi
title In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_short In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_full In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_fullStr In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_full_unstemmed In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
title_sort in-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
publishDate 2017
url http://irep.iium.edu.my/71912/
http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf
http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf
first_indexed 2023-09-18T21:41:58Z
last_indexed 2023-09-18T21:41:58Z
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